PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1763 [new locus tag: SAUSA300_RS09650 ]
• pan locus tag^?: SAUPAN004575000
• symbol: epiP
• pan gene symbol^?: epiP
• synonym:
• product: lantibiotic epidermin leader peptide processing serine protease EpiP

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1763 [new locus tag: SAUSA300_RS09650 ]
• symbol: epiP
• product: lantibiotic epidermin leader peptide processing serine protease EpiP
• replicon: chromosome
• strand: -
• coordinates: 1947842..1949215
• length: 1374
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914866 NCBI
• RefSeq: YP_494454 NCBI
• BioCyc: see SAUSA300_RS09650
• MicrobesOnline: 1293278 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  481
  541
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  841
  901
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  1021
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  1141
  1201
  1261
  1321
  
  ATGAAAATCATAAAGCGCGCTATTATTAGTTTAATAATATTGAGTCTTTTAATATCTATT
  ACAATGAGTAATGCATCAGCGTCAGAAGAACTATATTACAGTGTTGAATACAAAAATACA
  GCAACATTTAACAAGTTAGTTAAAAAGAAGTCCTTAAACGTTGTCTATAATATACCGGAA
  TTACATGTGGCACAGATTAAAATGACGAAAATGCATGCTAATGCTTTAGCAAACTATAAA
  AATGATATTAAATATATCAATGCCACATGTTCAACTTGTATTACTAGCGAGAAAACAATA
  GACAGAACATCAAATGAGTCATTATTTTCAAGACAATGGGATATGAATAAAATAACCAAT
  AATGGTGCATCGTATGATGATTTGCCAAAACATGCTAACACCAAAATAGCAATCATAGAT
  ACAGGTGTGATGAAAAACCATGACGATTTGAAAAATAATTTCTCGACTGATTCTAAAAAT
  TTAGTACCTTTAAACGGTTTTAGAGGTACTGAACCGGAGGAAACAGGTGATGTTCACGAT
  GTCAATGATAGGAAAGGACATGGCACGATGGTGTCGGGTCAAACGAGTGCTAATGGTAAG
  TTAATAGGTGTTGCACCGAATAACAAATTTACAATGTATCGCGTGTTTGGTAGTAAAAAA
  ACAGAACTGCTTTGGGTATCAAAAGCGATTGTTCAAGCTGCAAATGATGGAAATCAAGTC
  ATTAATATTAGTGTTGGTAGTTATATTATTTTGGACAAAAATGACCATCAAACATTTAGA
  AAAGATGAAAAAGTAGAATACGATGCGTTACAGAAAGCAATCAATTACGCCAAGAAGAAA
  AAATCTATCGTTGTTGCTGCAGCTGGTAATGATGGTATTGATGTCAATGACAAACAGAAA
  CTAAAATTACAGCGTGAATATCAAGGTAATGGCGAAGTGAAAGATGTTCCTGCATCTATG
  GACAATGTCGTTACAGTAGGATCTACAGATCAAAAGAGTAATCTATCTGAGTTTTCCAAT
  TTTGGTATGAATTATACAGATATTGCTGCGCCCGGAGGATCATTTGCTTATTTAAATCAA
  TTCGGTGTGGATAAATGGATGAATGAAGGGTATATGCATAAGGAGAACATTTTAACTACT
  GCCAATAACGGAAGATATATTTATCAAGCTGGAACTTCATTAGCCACACCTAAAGTTTCG
  GGAGCACTAGCTTTAATCATTGATAAATATCATCTTGAAAAACATCCAGATAAAGCGATT
  GAATTGTTATATCAGCATGGGACATCTAAGAATAATAAACCATTTAGTAGATATGGGCAT
  GGTGAGCTTGATGTGTATAAAGCATTAAATGTAGCAAATCAAAAAGCAAGTTAA
  60
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  1374
  

⊟Protein

• 3QFH
  PDB
• 3T41
  PDB

Protein Data Bank: 3QFH

Protein Data Bank: 3T41

⊟General

• locus tag: SAUSA300_1763 [new locus tag: SAUSA300_RS09650 ]
• symbol: EpiP
• description: lantibiotic epidermin leader peptide processing serine protease EpiP
• length: 457
• theoretical pI: 9.48761
• theoretical MW: 50735.1
• GRAVY: -0.497812

⊟Function

• reaction:
  EC 3.4.21.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion-associated serine protease mycosin (TIGR03921; EC 3.4.21.-; HMM-score: 136)
  Genetic information processing Protein fate Protein modification and repair type VII secretion-associated serine protease mycosin (TIGR03921; EC 3.4.21.-; HMM-score: 136)
  and 1 more
  cyanobactin maturation protease, PatA/PatG family (TIGR03895; EC 3.4.21.-; HMM-score: 50.3)
• TheSEED  :
  • Epidermin leader peptide processing serine protease epiP precursor (EC 3.4.21.-)
• PFAM:
  no clan defined Peptidase_S8; Subtilase family (PF00082; HMM-score: 146.4)
  and 2 more
  DUF2850; Protein of unknown function (DUF2850) (PF11012; HMM-score: 13.1)
  DUF2960; Protein of unknown function (DUF2960) (PF11173; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.73
  • Internal Helices: 0
• LocateP: Secretory(released) (with CS)
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: SNASASEE
• SignalP: Signal peptide SP(Sec/SPI) length 27 aa
  • SP(Sec/SPI): 0.997615
  • TAT(Tat/SPI): 0.000491
  • LIPO(Sec/SPII): 0.001085
  • Cleavage Site: CS pos: 27-28. ASA-SE. Pr: 0.9602
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 87162241 NCBI
• RefSeq: YP_494454 NCBI
• UniProt: A0A0H2XIS6 UniProt

⊟Protein sequence

• MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFNKLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDRTSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKNLVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGSKKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINYAKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSNLSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGTSLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALNVANQKAS

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: epiG < epiE < epiF < epiP < epiD < epiC < epiB < epiA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications