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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1874 [new locus tag: SACOL_RS09615 ]
  • pan locus tag?: SAUPAN004575000
  • symbol: epiP
  • pan gene symbol?: epiP
  • synonym:
  • product: epidermin leader peptide processing serine protease EpiP

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1874 [new locus tag: SACOL_RS09615 ]
  • symbol: epiP
  • product: epidermin leader peptide processing serine protease EpiP
  • replicon: chromosome
  • strand: -
  • coordinates: 1925673..1927046
  • length: 1374
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAAATCATAAAGCGCGCTATTATTAGTTTAATAATATTGAGTCTTTTAATATCTATT
    ACAATGAGTAATGCATCAGCGTCAGAAGAACTATATTACAGTGTTGAATACAAAAATACA
    GCAACATTTAACAAGTTAGTTAAAAAGAAGTCCTTAAACGTTGTCTATAATATACCGGAA
    TTACATGTGGCACAGATTAAAATGACGAAAATGCATGCTAATGCTTTAGCAAACTATAAA
    AATGATATTAAATATATCAATGCCACATGTTCAACTTGTATTACTAGCGAGAAAACAATA
    GACAGAACATCAAATGAGTCATTATTTTCAAGACAATGGGATATGAATAAAATAACCAAT
    AATGGTGCATCGTATGATGATTTGCCAAAACATGCTAACACCAAAATAGCAATCATAGAT
    ACAGGTGTGATGAAAAACCATGACGATTTGAAAAATAATTTCTCGACTGATTCTAAAAAT
    TTAGTACCTTTAAACGGTTTTAGAGGTACTGAACCGGAGGAAACAGGTGATGTTCACGAT
    GTCAATGATAGGAAAGGACATGGCACGATGGTGTCGGGTCAAACGAGTGCTAATGGTAAG
    TTAATAGGTGTTGCACCGAATAACAAATTTACAATGTATCGCGTGTTTGGTAGTAAAAAA
    ACAGAACTGCTTTGGGTATCAAAAGCGATTGTTCAAGCTGCAAATGATGGAAATCAAGTC
    ATTAATATTAGTGTTGGTAGTTATATTATTTTGGACAAAAATGACCATCAAACATTTAGA
    AAAGATGAAAAAGTAGAATACGATGCGTTACAGAAAGCAATCAATTACGCCAAGAAGAAA
    AAATCTATCGTTGTTGCTGCAGCTGGTAATGATGGTATTGATGTCAATGACAAACAGAAA
    CTAAAATTACAGCGTGAATATCAAGGTAATGGCGAAGTGAAAGATGTTCCTGCATCTATG
    GACAATGTCGTTACAGTAGGATCTACAGATCAAAAGAGTAATCTATCTGAGTTTTCCAAT
    TTTGGTATGAATTATACAGATATTGCTGCGCCCGGAGGATCATTTGCTTATTTAAATCAA
    TTCGGTGTGGATAAATGGATGAATGAAGGGTATATGCATAAGGAGAACATTTTAACTACT
    GCCAATAACGGAAGATATATTTATCAAGCTGGAACTTCATTAGCCACACCTAAAGTTTCG
    GGAGCACTAGCTTTAATCATTGATAAATATCATCTTGAAAAACATCCAGATAAAGCGATT
    GAATTGTTATATCAGCATGGGACATCTAAGAATAATAAACCATTTAGTAGATATGGGCAT
    GGTGAGCTTGATGTGTATAAAGCATTAAATGTAGCAAATCAAAAAGCAAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1374

⊟Protein[edit | edit source]

Protein Data Bank: 3QFH
Protein Data Bank: 3T41

⊟General[edit | edit source]

  • locus tag: SACOL1874 [new locus tag: SACOL_RS09615 ]
  • symbol: EpiP
  • description: epidermin leader peptide processing serine protease EpiP
  • length: 457
  • theoretical pI: 9.48761
  • theoretical MW: 50735.1
  • GRAVY: -0.497812

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion-associated serine protease mycosin (TIGR03921; EC 3.4.21.-; HMM-score: 136)
    Genetic information processing Protein fate Protein modification and repair type VII secretion-associated serine protease mycosin (TIGR03921; EC 3.4.21.-; HMM-score: 136)
    and 1 more
    cyanobactin maturation protease, PatA/PatG family (TIGR03895; EC 3.4.21.-; HMM-score: 50.3)
  • TheSEED  :
    • Epidermin leader peptide processing serine protease epiP precursor (EC 3.4.21.-)
  • PFAM:
    no clan defined Peptidase_S8; Subtilase family (PF00082; HMM-score: 146.4)
    and 2 more
    DUF2850; Protein of unknown function (DUF2850) (PF11012; HMM-score: 13.1)
    DUF2960; Protein of unknown function (DUF2960) (PF11173; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: SNASASEE
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.997615
    • TAT(Tat/SPI): 0.000491
    • LIPO(Sec/SPII): 0.001085
    • Cleavage Site: CS pos: 27-28. ASA-SE. Pr: 0.9602
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFNKLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDRTSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKNLVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGSKKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINYAKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSNLSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGTSLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALNVANQKAS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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