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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02170
  • pan locus tag?: SAUPAN005022000
  • symbol: SAOUHSC_02170
  • pan gene symbol?: —
  • synonym:
  • product: peptidoglycan hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02170
  • symbol: SAOUHSC_02170
  • product: peptidoglycan hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2034690..2034986
  • length: 297
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    GTGCCAGCAGGTTATACACTCGATAAAAACAATGTACCGTATAAAAAAGAGACTGGTTAT
    TACACAGTTGCCAATGTTAAAGGTAATAACGTGAGGGATGGCTATTCAACTAATTCAAGA
    ATTACAGGTGTATTACCCAATAACGCAACTATCAAATATGACGGCGCATATTGCATTAAT
    GGCTATAGATGGATTACTTATATTGCTAATAGTGGACAACGTCGTTATATAGCGACAGGA
    GAGGTAGACAAGGCAGGTAATAGAATAAGCAGTTTTGGTAAGTTTAGTGCAGTTTGA
    60
    120
    180
    240
    297

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02170
  • symbol: SAOUHSC_02170
  • description: peptidoglycan hydrolase
  • length: 98
  • theoretical pI: 9.95644
  • theoretical MW: 10832
  • GRAVY: -0.590816

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Phage endolysin N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
  • ⊞PFAM:
    SH3 (CL0010) SH3_5; Bacterial SH3 domain (PF08460; HMM-score: 34.8)
    and 1 more
    SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 21.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • ⊞DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0.0021
    • Extracellular Score: 0.9978
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.183786
    • TAT(Tat/SPI): 0.003498
    • LIPO(Sec/SPII): 0.01098
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPAGYTLDKNNVPYKKETGYYTVANVKGNNVRDGYSTNSRITGVLPNNATIKYDGAYCINGYRWITYIANSGQRRYIATGEVDKAGNRISSFGKFSAV

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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