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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02180
  • pan locus tag?: SAUPAN005045000
  • symbol: SAOUHSC_02180
  • pan gene symbol?: —
  • synonym:
  • product: phage minor structural protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02180
  • symbol: SAOUHSC_02180
  • product: phage minor structural protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2038746..2042531
  • length: 3786
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    GTGATACATGTTTTAGATTTTAACGACAAGATTATAGATTTCCTTTCTACTGATGACCCT
    TCCTTAGTTAGAGCGATTCATAAACGTAATGTTAATGACAATTCAGAAATGCTTGAACTG
    CTCATATCATCAGAAAGAGCTGAAAAGTTCCGTGAACGACATCGTGTTATTATAAGGGAT
    TCAAACAAACAATGGCGTGAATTTATTATTAACTGGGTTCAAGATACGATGGACGGCTAC
    ACAGAGATAGAATGTATAGCGTCTTATCTTGCTGATATAACAACAGCTAAACCGTATGCA
    CCAGGCAAATTTGAGAAAAAGACAACTTCAGAAGCATTGAAAGATGTGTTGAGCGATACA
    GGTTGGGAAGTTTCTGAACAAACCGAATACGATGGCTTACGTACTACGTCATGGACTTCT
    TATCAAACTAGATATGAAGTTTTAAAGCAATTATGTACAACCTATAAAATGGCATTGGAT
    TTTTATATAGAGCTTAGTTCTAATACCGTCAAAGGTAGATATGTGGTACTCAAAAAGAAA
    AACAGCTTATTCAAAGGTAAAGAAATTGAGTATGGTAAAGATTTGGTTGGGTTAACTAGG
    AAGATTGATATGTCAGAAATCAAAACAGCATTAATTGCTGTGGGACCCGAAAATGACAAA
    GGAAAGCGTTTAGAGTTAGTTGTGACTGATGACGAAGCACAAAGTCAATTCAACTTACCT
    ACCCGTTATATTTGGGGAATATACGAACCTCAATCAGATGATCAAAATATGAATGAAACA
    CGGTTGCGTTCTTTAGCCAAAACAGAGTTAAATAAACGTAAGTCGGCAGTTATGTCATAT
    GAGATTACTTCTACTGATTTGGAAGTTACGTATCCGCACGAGATTATATCAATTGGTGAT
    ACAGTCAGAGTAAAACATAGAGATTTTAACCCGCCATTGTATGTAGAGGCAGAAGTTATT
    GCCGAAGAATATAACATAATTTCAGAAAATAGCACATATACATTCGGTCAACCTAAAGAG
    TTCAAAGAATCAGAATTACGAGAAGAGTTTAACAAACGATTGAACATAATACATCAAAAG
    TTAAACGATAATATTAGCAATATCAACACTATAGTTAAAGATGTTGTAGATGGTGAATTA
    GAATACTTTGAACGCAAAATACACAAAAATGATACACCGCCAGAAAATCCAGTCAATGAT
    ATGCTTTGGTATGATACAAGTAACCCTGATGTTGCTGTCTTGCGTAGATATTGGAATGGT
    CGATGGATTGAAGCAACACCAAATGATGTTGAAAAATTAGGTGGTATAACAAGAGAGAAA
    GCGCTATTCAGTGAATTAAACAATATTTTTATTAATTTATCTATACAACACGCTAGTCTT
    TTGTCAGAAGCTACAGAATTACTGAATAGCGAGTACTTAGTAGATAATGATTTGAAAGCG
    GACTTACAAGCAAGTTTAGACGCTGTGATTGATGTTTATAATCAAATTAAAAATAATTTA
    GAATCTATGACACCCGAAACTGCAACGATTGGTCGGTTGGTAGATACACAAGCTTTATTT
    CTTGAATATAGAAAGAAATTACAAGATGTCTATACAGATGTAGAAGATGTCAAAATCGCT
    ATTTCAGATAGATTTAAATTATTACAGTCACAATACACTGATGAAAAATATAAAGAAGCG
    TTGGAAATAATAGCAACAAAATTTGGTTTAACGGTGAATGAAGATTTGCAGTTAGTCGGA
    GAACCTAATGTTGTTAAATCAGCTATTGAAGCAGCTAGAGAATCCACAAAAGAACAATTA
    CGTGACTATGTAAAAACATCGGACTATAAAACAGACAAAGACGGTATTGTTGAACGTTTA
    GATACTGCTGAAGCTGAGAGAACGACTTTAAAAGGTGAAATCAAAGATAAAGTTACGTTA
    AACGAATATCGAAACGGATTGGAAGAACAAAAACAATATACTGATGACCAGTTAAGTGAT
    TTGTCCAATAATCCTGAGATTAAAGCAAGTATTGAACAAGCAAATCAAGAAGCGCAAGAA
    GCTTTAAAATCATACATTGATGCTCAAGATAATCTTAAAGAGAAGGAATCGCAAGCGTAT
    GCTGATGGTAAAATTTCGGAAGAAGAGCAACGCGCTATACAAGATGCTCAAGCTAAACTT
    GAAGAGGCAAAACAAAACGCAGAACTAAAGGCTAGAAACGCTGAAAAGAAAGCTAATGCT
    TATACAGACAACAAGGTCAAAGAAAGCACAGATGCACAGAGGAGAACACTGACTCGCTAT
    GGTTCTCAAATTATACAAAATGGTAAGGAAATCAAATTAAGAACTACTAAAGAAGAGTTT
    AATGCAACCAATCGTACACTTTCAAATATATTAAACGAGATTGTCCAAAACGTTACAGAT
    GGAACAACAATCAGATATGATGATAACGGAGTGGCTCAAGCTTTAAATGTGGGGCCACGT
    GGTATTAGATTAAATGCTGATAAAATTGATATTAACGGTAATAGAGAAATAAACCTTCTT
    ATCCAAAATATGCGAGATAAAGTAGATAAAACCGATATTGTCAACAGCCTTAATTTATCA
    AGAGAGGGTCTTGATATCAATGTTAATAGAATTGGAATTAAAGGCGGTAACAATAACAGA
    TATGTTCAAATACAGAATGATTCTATTGAACTAGGTGGTATTGTGCAACGAACTTGGAAA
    GGCAAACGATCAACCGATGATATATTCACACGTCTTAAAGATGGACATCTAAGGTTTAGA
    AATAATACCGCAGGCGGTTCACTTTATATGTCACATTTTGGTATTTCAACATATATTGAT
    GGAGAAGGCGAAGACGGAGGTTCATCCGGTACTATTCAATGGTGGGATAAAACTTACAGT
    GATAGCGGTATGAATGGCATAACAATCAATTCCTATGGTGGTGTCGTTGCACTAACGTCA
    GATAATAATCGGGTTGTTCTGGAGTCTTACGCTTCATCGAATATCAAAAGCAAACAGGCA
    CCGGTGTATTTATATCCAAACACAGACAAAGTGCCTGGATTAAACCGATTTGCATTCACG
    CTGTCTAATGCAGATAACGCTTATTCGAGTGATGGTTATATTATGTTTGGTTCTGATGAG
    AACTATGATTACGGTGCGGGTATCAGGTTTTCTAAAGAAAGAAATAAAGGTCTTGTTCAA
    ATTGTTAATGGACGATATGCAACAGGTGGAGATACAACAATCGAAGCAGGGTATGGCAAA
    TTTAATATGCTGAAACGACGTGATGGTAATAGGTATATTCATATACAGAGTACAGACCTA
    CTGTCTGTAGGTTCAGATGATGCAGGAGATAGGATAGCTTCTAACTCAATTTATAGACGT
    ACTTATTCGGCCGCAGCTAATTTGCATATTACTTCTGCTGGCACAATTGGGCGTTCGACA
    TCAGCGCGTAAATACAAGTTATCTATCGAAAATCAATATAACGATAGAGATGAACAACTG
    GAACATTCAAAAGCTATTCTTAACTTACCTATTAGAACGTGGTTTGATAAAGCTGAGTCT
    GAAATTTTAGCTAGAGAGCTGAGAGAAGATAGAAAATTATCGGAAGACACCTATAAACTT
    GATAGATACGTAGGTTTGATTGCTGAAGAGGTGGAGAATTTAGGATTAAAAGAGTTTGTC
    ACGTATGATGACAAAGGAGAAATTGAAGGTATAGCGTATGATCGTCTATGGATTCATCTT
    ATCCCTGTTATCAAAGAACAACAACTAAGAATCAAGAAATTGGAGGAGTCAAAGAATGCA
    GGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3786

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02180
  • symbol: SAOUHSC_02180
  • description: phage minor structural protein
  • length: 1261
  • theoretical pI: 4.89409
  • theoretical MW: 143742
  • GRAVY: -0.700555

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage minor structural protein, N-terminal region (TIGR01665; HMM-score: 316.1)
    and 2 more
    Cellular processes Cellular processes Pathogenesis type III secretion apparatus protein, YscD/HrpQ family (TIGR02500; HMM-score: 11.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type III secretion apparatus protein, YscD/HrpQ family (TIGR02500; HMM-score: 11.8)
  • TheSEED  :
    • Structural protein, phage associated
  • PFAM:

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001686
    • TAT(Tat/SPI): 0.000088
    • LIPO(Sec/SPII): 0.000339
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISSERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPGKFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMALDFYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPENDKGKRLELVVTDDEAQSQFNLPTRYIWGIYEPQSDDQNMNETRLRSLAKTELNKRKSAVMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYTFGQPKEFKESELREEFNKRLNIIHQKLNDNISNINTIVKDVVDGELEYFERKIHKNDTPPENPVNDMLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIFINLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETATIGRLVDTQALFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIATKFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAEAERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEALKSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANAYTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNVTDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNSLNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKDGHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSYGGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSSDGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNMLKRRDGNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYKLSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYVGLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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