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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02191
  • pan locus tag?: SAUPAN001539000
  • symbol: SAOUHSC_02191
  • pan gene symbol?: —
  • synonym:
  • product: HK97 family phage major capsid protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02191
  • symbol: SAOUHSC_02191
  • product: HK97 family phage major capsid protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2052003..2053250
  • length: 1248
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAAACAAAAGAAGAGTTACAATCTGAGATTTCAGACATTAAAAGACAAATTGATTTA
    AAGGTGAAGTATGCAACGAGAGCACTTAATAACGATGAGTTAGAAAAAGCAGAAAAATTA
    GAACAAGAAATTACTGATTTACGTTCTCAAATCCAAGAAAAACAAGAAGAATTAGATAAG
    CTAAAAGAAAAAGATGGAACTTCAGAAAACAATCAACAATCAGTGGAAGTAAACGAAGCA
    AGTACTTATCGAAATCAAGCAAACATTAATGATTTAGGTATTTCGATTCAAAACACAAAG
    GTAACATCACAAGAAGTTAGAGATTTTACTGAATATCTTGAAACACGCAATGATATTCAA
    GGTGGTTCGTTAAAAACAGACTCAGGATTTGTAGTTATTCCAGAGGAAATTGTTACAGAT
    ATTTTAAAATTAAAAGAGGTTGAGTTTAATCTTGATAAGTATGTGACGGTCAAACGTGTT
    ACAAATGGTTCTGGTAAATATCCGGTAGTACGACAATCAGAAGTTGCAGCCCTTGAAAAA
    GTTGAAGAATTAGAAGAAAACCCTGAATTAGCAGTTAAACCATTCTTCCAATTAGCATAT
    GACATTAATACACACCGTGGTTACTTCCGAATTTCACGTGAAGCAATCGAAGATGCAAAA
    GTGAATGTTTTGCAAGAATTGAAACTATGGATGGCGCGAACTATTGCAGCAACACGAAAC
    AAAGCAATTATTGATGTTATCACTAAAGGATCAACGGGTTCTACAAGTTCAGGTTTTGAA
    AAAGAAGGCAAGAAATTAGAAGTTAAAAAAGCAAAATCTTTAGATGATATTAAAGATGCT
    ATTAACCTGAATGTTAAGCCAAATTACGAACATAATGTTGCGATTGTTTCGCAAACTATG
    TTTGCAAAATTAGACAAAATGAAAGATAAGCTAGGAAACTATTTAATCCAGCCAGATGTT
    AAAGAAAAAACGCAACAGCGTTTATTAGGAGCTAAAATCGAAATTTTACCTGATGAAGTA
    CTAGGGCAAAAAGGTAATAACACTTTGATTATCGGTAACTTAAAAGATGCGATTGTTTTA
    TTTGACCGCTCTCAATACCAAGCATCATGGACTGACTACATGCATTTCGGAGAATGTTTA
    ATGATTGCTGTACGTCAAGACTGTAGAATTCTAGATTATAAATCAGCAATTGTGATTGAA
    TATGATGATAGTGAACGCGGTGAAGGCGATCTTGGCTTAGAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1248

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02191
  • symbol: SAOUHSC_02191
  • description: HK97 family phage major capsid protein
  • length: 415
  • theoretical pI: 4.78474
  • theoretical MW: 47181
  • GRAVY: -0.60506

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage major capsid protein, HK97 family (TIGR01554; HMM-score: 177.8)
    and 2 more
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(hydroxyalkanoate) granule-associated protein (TIGR01837; HMM-score: 10.8)
    SH3 domain protein (TIGR04211; HMM-score: 9.2)
  • TheSEED  :
    • Phage major capsid protein
  • PFAM:
    Phage-coat (CL0373) Phage_capsid; Phage capsid family (PF05065; HMM-score: 136.6)
    and 13 more
    no clan defined Trimer_CC; Trimerisation motif (PF08954; HMM-score: 14.5)
    DUF2203; Uncharacterized conserved protein (DUF2203) (PF09969; HMM-score: 11.7)
    Mito_fiss_reg; Mitochondrial fission regulator (PF05308; HMM-score: 10.3)
    MBB (CL0193) DUF3573; Protein of unknown function (DUF3573) (PF12097; HMM-score: 10)
    no clan defined FlxA; FlxA-like protein (PF14282; HMM-score: 10)
    XLF; XLF-Cernunnos, XRcc4-like factor, NHEJ component (PF09302; HMM-score: 9.7)
    DUF1359; Protein of unknown function (DUF1359) (PF07097; HMM-score: 9.6)
    IncA; IncA protein (PF04156; HMM-score: 9.5)
    CCDC-167; Coiled-coil domain-containing protein 167 (PF15188; HMM-score: 8.6)
    Spc24; Spc24 subunit of Ndc80 (PF08286; HMM-score: 7.8)
    V_ATPase_I; V-type ATPase 116kDa subunit family (PF01496; HMM-score: 7.6)
    LUC7; LUC7 N_terminus (PF03194; HMM-score: 6.8)
    Med9; RNA polymerase II transcription mediator complex subunit 9 (PF07544; HMM-score: 6.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00521
    • TAT(Tat/SPI): 0.0076
    • LIPO(Sec/SPII): 0.00115
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKTKEELQSEISDIKRQIDLKVKYATRALNNDELEKAEKLEQEITDLRSQIQEKQEELDKLKEKDGTSENNQQSVEVNEASTYRNQANINDLGISIQNTKVTSQEVRDFTEYLETRNDIQGGSLKTDSGFVVIPEEIVTDILKLKEVEFNLDKYVTVKRVTNGSGKYPVVRQSEVAALEKVEELEENPELAVKPFFQLAYDINTHRGYFRISREAIEDAKVNVLQELKLWMARTIAATRNKAIIDVITKGSTGSTSSGFEKEGKKLEVKKAKSLDDIKDAINLNVKPNYEHNVAIVSQTMFAKLDKMKDKLGNYLIQPDVKEKTQQRLLGAKIEILPDEVLGQKGNNTLIIGNLKDAIVLFDRSQYQASWTDYMHFGECLMIAVRQDCRILDYKSAIVIEYDDSERGEGDLGLEA

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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