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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02194
  • pan locus tag?: SAUPAN001537000
  • symbol: SAOUHSC_02194
  • pan gene symbol?:
  • synonym:
  • product: HK97 family phage portal protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02194
  • symbol: SAOUHSC_02194
  • product: HK97 family phage portal protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2053915..2055105
  • length: 1191
  • essential: no DEG

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGATGGTTCAAACTTTGCCAGGTTTTCAAGGAACAAAATTACGACAATATAAAGATATA
    GAAGCAATTAGGCATAGCGACATCTTTACGGCAGTTATGATGATTGCTTCTGATTTGGCG
    CGCATGCCAATTAGGGTGACAGTGAACGGCCAAATTAATTATAGTGACAGGATTGTTAAT
    TTGTTAAATACACGTCCTAACCCAATGTATAACGGCTATATATTCAAATTAGTAGTGTTT
    GTTAGTGCCTTACTAACATCGCACGGCTATATTGAAATTACACGTGATAAAACAGGAGAA
    CCTATGAATTTAACGTTCAGAAAGACATCCGAAATAGAATTGAAATCAGACGCAAGAGGT
    CGACTGTATTATTTTCATCAAAGGATAGACAGTAACGGAAATAATATAGAACGTAATGTT
    AAGTTTGAGGATATGCTAGACATCAAATTTTATTCGTTGGATGGTATAAATGGTTTGTCA
    CTGTTAGACACATTAAGTCGCACGATAGAATCAGATAACAATGGAAAAGATTTCCTTAAT
    AATTTCTTGCGAAATGGCACACATGCTGGTGGTATTTTGAAAATGAAAGGTGTATTAGAT
    AATAAAAAAGCAAGAGACCGTGCCAGAGAAGAATTTCACAAAAGTTTTAGTGGAACTAAA
    CAAGCTGGGAAAGTTGTCGTACTCGATGAATCAATGACGTTTGATCAATTAGAAGTTGAT
    ACAGAAGTTTTAAAGCTTATCAGAGAAAACAAATCATCAACAAGAGAAATAGCAGGTGTA
    TTTGGTATTCCATTGCATAAGTTCGGCATAGAAACAGCGAACATGAGTATCACGGATGCT
    AATTTAGATTACTTATCAACTTTAAAACCTTATATTACATGCGTTTGTGCAGAATTGAAT
    TTTAAGTTTAATGATGAATATGTGAATCGTGAATTTAAATTTGATACCACTGAAATACGA
    GTTGTTGATGAAAAAACACAAGCTGAAATTGACAAAATTAACATTGATTCTGGAAAGATG
    AATATCGATGAAATTAGACAACGTGATGGATTAGCGCCAATACCAGGCGGTAATGGTAGC
    ATTCACAGAGTCGATTTAAACCATGTAAATATTGAACTTGTAGATGAGTATCAGATGAAT
    AAATCGAGAGCTACTGATAAAAAATTGAAAGGTGGTGAGGAAAATGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1191

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_02194
  • symbol: SAOUHSC_02194
  • description: HK97 family phage portal protein
  • length: 396
  • theoretical pI: 6.53045
  • theoretical MW: 45104
  • GRAVY: -0.480556

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage portal protein, HK97 family (TIGR01537; HMM-score: 369.5)
  • TheSEED  :
    • Phage portal protein
  • PFAM:
    no clan defined Phage_portal; Phage portal protein (PF04860; HMM-score: 215.3)
    and 1 more
    Phage_TACs (CL0567) Phage_TAC_12; Phage tail assembly chaperone protein, TAC (PF12363; HMM-score: 14.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00974
    • TAT(Tat/SPI): 0.001127
    • LIPO(Sec/SPII): 0.001541
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MMVQTLPGFQGTKLRQYKDIEAIRHSDIFTAVMMIASDLARMPIRVTVNGQINYSDRIVNLLNTRPNPMYNGYIFKLVVFVSALLTSHGYIEITRDKTGEPMNLTFRKTSEIELKSDARGRLYYFHQRIDSNGNNIERNVKFEDMLDIKFYSLDGINGLSLLDTLSRTIESDNNGKDFLNNFLRNGTHAGGILKMKGVLDNKKARDRAREEFHKSFSGTKQAGKVVVLDESMTFDQLEVDTEVLKLIRENKSSTREIAGVFGIPLHKFGIETANMSITDANLDYLSTLKPYITCVCAELNFKFNDEYVNREFKFDTTEIRVVDEKTQAEIDKINIDSGKMNIDEIRQRDGLAPIPGGNGSIHRVDLNHVNIELVDEYQMNKSRATDKKLKGGEENE

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]