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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02311
  • pan locus tag?: SAUPAN005348000
  • symbol: SAOUHSC_02311
  • pan gene symbol?: kdpB
  • synonym:
  • product: potassium-transporting ATPase subunit B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02311
  • symbol: SAOUHSC_02311
  • product: potassium-transporting ATPase subunit B
  • replicon: chromosome
  • strand: -
  • coordinates: 2140452..2142425
  • length: 1974
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAGTTTTTATAAGCTGAATCTTAAACAATTAATAAAAAATCCGATAATGTTTGTCGTT
    GAGGTTGGAATGATTTTAACGTTGATATTAATATGTTTTCCTGACATATTTGGAACTAGC
    TATCTATCGCGAGGTTATTTGATTACAATTTTTATCATATTATTAATAACTATATTGTTT
    GCTAATTTTTCAGAAGCCTTTGCTGAAGGTCGTGGAAAAGCGCAAGCTGATAGTTTAAGA
    CAAGCACAATCAAATTTAACTGCTCGATTGATAGAAGAAAATGGTGCGTATCGGATAGTG
    AACGCCACAGAGTTAAAAGCCGGACAAAATATTCGGGTAGAAAATGGAGAAACAATCCCT
    GCTGATGGCGTTGTAATAAATGGATTAGCAACCGTTGATGAATCTGCAATTACTGGTGAG
    TCTGCACCTGTGATAAAAGAATCAGGTGGCGATTTTGATGGAGTCATTGGCGGTACACTT
    GTAACATCTGATTGGTTAGAAATACGTGTTGAGAGTGAAGCGGGTACATCTTTTTTAGAT
    AAAATGATTGCCTTAGTTGAAGGGGCGGAAAGAAATAAAACACCAAATGAAATTGCACTT
    TTTACATTATTAACAACGTTAACAATTATATTTTTAGTCGTTATTGTAACGTTATATCCA
    ATAGCATCTTACCTGCATTTAATTTTACCTATTGCGATGCTCATTGCGTTAACAGTATGT
    TTAATACCAACGACAATCGGTGGTTTGTTATCGGCGATTGGTATTGCAGGCATGGATCGT
    GTTACTCAATTCAATGTATTAGCGAAAAGTGGGCGTGCTGTAGAAGTATGTGGAGATGTC
    GATGTAATGATTTTAGACAAGACAGGCACAATCACTTATGGCAATCGAATCGCTAGTGAA
    TTTTTACCAGTGAATCAACAAATGCTTGAAAAATTGATAGTCGCTGCTTATATGTCATCA
    ATCTACGATGATACGCCAGAAGGAAAAAGTATTGTACGATTAGCGAAGCAGATGTATATA
    AATGAGCTGCCTAAAGACATTGATGGCACATATAAGCCTTTTACAGCTGAAACTAGAATG
    AGCGGAATCATTACAAATGAAATATCGGTATTCAAAGGTGCACCTAATAGTATGATAAAT
    CTTGTCAAACAACAACAAGGCAATATTCCTCTTAATATTGAGTCTCTCTGTATGGATGTA
    TCGAGTAAAGGTGGCACACCATTAATAGTCATTGAAAACAATGTCATGCTTGGCGTGATT
    TATTTAAAAGATGTGATTAAAGATGGTCTTGTAGAACGATTTACTGAATTGAGAAAAATG
    GGGATTGAAACAGTTATGTGTACAGGTGATAATGCTTTAACAGCAGCTACAATTGCAAAA
    GAAGCCGGGGTTGATAGATTTGTTGCAGAGTGTAAACCCGAAGATAAAATTAAAGTCATC
    AAAGATGAACAAGCGAAAGGCCATATTGTTGCGATGACAGGAGATGGTACAAATGATGCA
    CCTGCGCTAGCACAAGCGAATATAGGATTAGCTATGAACTCAGGGACAATTAGTGCTAAA
    GAGGCAGCTAATCTAATTGATTTAGATTCGAATCCTACAAAGTTAATAGAGGTTGTAAAA
    ATAGGCAAACAATTATTAATGACGAGAGGTGCATTAACGACATTTAGTTTGGCTAATGAC
    GTAGCAAAATACTTTGCAATTTTACCTGCACTAATGATGTCAACAATTCCAGAAATGACT
    TCACTAAATATTATGCATTTATCGTCACCTAAATCTGCAATTATATCAGCTTTGATATTC
    AATGCATTAATCATTGTTGCCTTAATTCCAATTGCCATGAAAGGTGTAAAAGTAAAGGGT
    TACAGCATTGATAGAATCTTTATAAATAATATGTTGATTTATGGATTAGGTGGATTAATC
    GTCCCGTTTTTAGGCATTAAATTAATAGATATGATTGTACAATTTTTTGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1974

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02311
  • symbol: SAOUHSC_02311
  • description: potassium-transporting ATPase subunit B
  • length: 657
  • theoretical pI: 4.87663
  • theoretical MW: 70967.1
  • GRAVY: 0.446119

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.3.12?  ExPASy
    Potassium-transporting ATPase ATP + H2O + K+(Out) = ADP + phosphate + K+(In)
  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds K+-transporting ATPase, B subunit (TIGR01497; EC 3.6.3.12; HMM-score: 976)
    and 18 more
    heavy metal translocating P-type ATPase (TIGR01525; EC 3.6.3.-; HMM-score: 296.5)
    HAD ATPase, P-type, family IC (TIGR01494; EC 3.6.3.-; HMM-score: 262.5)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cadmium-translocating P-type ATPase (TIGR01512; EC 3.6.3.3; HMM-score: 219.5)
    Cellular processes Cellular processes Detoxification copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 217.8)
    Metabolism Transport and binding proteins Cations and iron carrying compounds copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 217.8)
    plasma-membrane proton-efflux P-type ATPase (TIGR01647; EC 3.6.3.6; HMM-score: 162.2)
    calcium-translocating P-type ATPase, PMCA-type (TIGR01517; EC 3.6.3.8; HMM-score: 118.1)
    Metabolism Transport and binding proteins Cations and iron carrying compounds calcium-translocating P-type ATPase, SERCA-type (TIGR01116; EC 3.6.3.8; HMM-score: 102.2)
    calcium-transporting P-type ATPase, PMR1-type (TIGR01522; EC 3.6.3.8; HMM-score: 100.6)
    P-type ATPase of unknown pump specificity (type V) (TIGR01657; HMM-score: 100)
    Metabolism Energy metabolism ATP-proton motive force interconversion Na,H/K antiporter P-type ATPase, alpha subunit (TIGR01106; EC 3.6.3.-; HMM-score: 92.6)
    potassium/sodium efflux P-type ATPase, fungal-type (TIGR01523; EC 3.6.3.-; HMM-score: 87.1)
    Metabolism Transport and binding proteins Cations and iron carrying compounds magnesium-translocating P-type ATPase (TIGR01524; EC 3.6.3.2; HMM-score: 83.4)
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 25.2)
    phospholipid-translocating P-type ATPase, flippase (TIGR01652; EC 3.6.3.1; HMM-score: 23)
    Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 20.3)
    Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 18.5)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 13.2)
  • TheSEED  :
    • Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)
    Potassium metabolism Potassium metabolism - no subcategory Potassium homeostasis  Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)
  • PFAM:
    HAD (CL0137) Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 109.5)
    no clan defined E1-E2_ATPase; E1-E2 ATPase (PF00122; HMM-score: 108.7)
    and 2 more
    HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 26.6)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 14.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001774
    • TAT(Tat/SPI): 0.000132
    • LIPO(Sec/SPII): 0.009143
  • predicted transmembrane helices (TMHMM): 7

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSFYKLNLKQLIKNPIMFVVEVGMILTLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQSNLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESAPVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIALFTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGMDRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAAYMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGAPNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVERFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIVAMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMTRGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIVALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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