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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2067 [new locus tag: SACOL_RS10815 ]
  • pan locus tag?: SAUPAN005348000
  • symbol: kdpB
  • pan gene symbol?: kdpB
  • synonym:
  • product: potassium-transporting ATPase subunit B

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2067 [new locus tag: SACOL_RS10815 ]
  • symbol: kdpB
  • product: potassium-transporting ATPase subunit B
  • replicon: chromosome
  • strand: -
  • coordinates: 2129728..2131755
  • length: 2028
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCATCATGTAAATAAATATTTCAATCAAACAATGGTGATAGAAGCTTTGAAAATGAGT
    TTTTATAAGCTGAATCTTAAACAATTAATAAAAAATCCGATAATGTTTGTCGTTGAGGTT
    GGAATGATTTTAACGTTGATATTAATATGTTTTCCTGACATATTTGGAACTAGCTATCTA
    TCGCGAGGTTATTTGATTACAATTTTTATCATATTATTAATAACTATATTGTTTGCTAAT
    TTTTCAGAAGCCTTTGCTGAAGGTCGTGGAAAAGCGCAAGCTGATAGTTTAAGACAAGCA
    CAATCAAATTTAACTGCTCGATTGATAGAAGAAAATGGTGCGTATCGGATAGTGAACGCC
    ACAGAGTTAAAAGCCGGACAAAATATTCGGGTAGAAAATGGAGAAACAATCCCTGCTGAT
    GGCGTTGTAATAAATGGATTAGCAACCGTTGATGAATCTGCAATTACTGGTGAGTCTGCA
    CCTGTGATAAAAGAATCAGGTGGCGATTTTGATGGAGTCATTGGCGGTACACTTGTAACA
    TCTGATTGGTTAGAAATACGTGTTGAGAGTGAAGCGGGTACATCTTTTTTAGATAAAATG
    ATTGCCTTAGTTGAAGGGGCGGAAAGAAATAAAACACCAAATGAAATTGCACTTTTTACA
    TTATTAACAACGTTAACAATTATATTTTTAGTCGTTATTGTAACGTTATATCCAATAGCA
    TCTTACCTGCATTTAATTTTACCTATTGCGATGCTCATTGCGTTAACAGTATGTTTAATA
    CCAACGACAATCGGTGGTTTGTTATCGGCGATTGGTATTGCAGGCATGGATCGTGTTACT
    CAATTCAATGTATTAGCGAAAAGTGGGCGTGCTGTAGAAGTATGTGGAGATGTCGATGTA
    ATGATTTTAGACAAGACAGGCACAATCACTTATGGCAATCGAATCGCTAGTGAATTTTTA
    CCAGTGAATCAACAAATGCTTGAAAAATTGATAGTCGCTGCTTATATGTCATCAATCTAC
    GATGATACGCCAGAAGGAAAAAGTATTGTACGATTAGCGAAGCAGATGTATATAAATGAG
    CTGCCTAAAGACATTGATGGCACATATAAGCCTTTTACAGCTGAAACTAGAATGAGCGGA
    ATCATTACAAATGAAATATCGGTATTCAAAGGTGCACCTAATAGTATGATAAATCTTGTC
    AAACAACAACAAGGCAATATTCCTCTTAATATTGAGTCTCTCTGTATGGATGTATCGAGT
    AAAGGTGGCACACCATTAATAGTCATTGAAAACAATGTCATGCTTGGCGTGATTTATTTA
    AAAGATGTGATTAAAGATGGTCTTGTAGAACGATTTACTGAATTGAGAAAAATGGGGATT
    GAAACAGTTATGTGTACAGGTGATAATGCTTTAACAGCAGCTACAATTGCAAAAGAAGCC
    GGGGTTGATAGATTTGTTGCAGAGTGTAAACCCGAAGATAAAATTAAAGTCATCAAAGAT
    GAACAAGCGAAAGGCCATATTGTTGCGATGACAGGAGATGGTACAAATGATGCACCTGCG
    CTAGCACAAGCGAATATAGGATTAGCTATGAACTCAGGGACAATTAGTGCTAAAGAGGCA
    GCTAATCTAATTGATTTAGATTCGAATCCTACAAAGTTAATAGAGGTTGTAAAAATAGGC
    AAACAATTATTAATGACGAGAGGTGCATTAACGACATTTAGTTTGGCTAATGACGTAGCA
    AAATACTTTGCAATTTTACCTGCACTAATGATGTCAACAATTCCAGAAATGACTTCACTA
    AATATTATGCATTTATCGTCACCTAAATCTGCAATTATATCAGCTTTGATATTCAATGCA
    TTAATCATTGTTGCCTTAATTCCAATTGCCATGAAAGGTGTAAAAGTAAAGGGTTACAGC
    ATTGATAGAATCTTTATAAATAATATGTTGATTTATGGATTAGGTGGATTAATCGTCCCG
    TTTTTAGGCATTAAATTAATAGATATGATTGTACAATTTTTTGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2028

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL2067 [new locus tag: SACOL_RS10815 ]
  • symbol: KdpB
  • description: potassium-transporting ATPase subunit B
  • length: 675
  • theoretical pI: 5.09826
  • theoretical MW: 73152.7
  • GRAVY: 0.426667

Function[edit | edit source]

  • reaction:
    EC 3.6.3.12?  ExPASy
    Potassium-transporting ATPase ATP + H2O + K+(Out) = ADP + phosphate + K+(In)
  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds K+-transporting ATPase, B subunit (TIGR01497; EC 3.6.3.12; HMM-score: 987.7)
    and 18 more
    heavy metal translocating P-type ATPase (TIGR01525; EC 3.6.3.-; HMM-score: 296.5)
    HAD ATPase, P-type, family IC (TIGR01494; EC 3.6.3.-; HMM-score: 262.4)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cadmium-translocating P-type ATPase (TIGR01512; EC 3.6.3.3; HMM-score: 219.5)
    Cellular processes Cellular processes Detoxification copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 217.9)
    Metabolism Transport and binding proteins Cations and iron carrying compounds copper-translocating P-type ATPase (TIGR01511; EC 3.6.3.4; HMM-score: 217.9)
    plasma-membrane proton-efflux P-type ATPase (TIGR01647; EC 3.6.3.6; HMM-score: 162.2)
    calcium-translocating P-type ATPase, PMCA-type (TIGR01517; EC 3.6.3.8; HMM-score: 120.2)
    Metabolism Transport and binding proteins Cations and iron carrying compounds calcium-translocating P-type ATPase, SERCA-type (TIGR01116; EC 3.6.3.8; HMM-score: 102.5)
    calcium-transporting P-type ATPase, PMR1-type (TIGR01522; EC 3.6.3.8; HMM-score: 100.6)
    P-type ATPase of unknown pump specificity (type V) (TIGR01657; HMM-score: 100)
    Metabolism Energy metabolism ATP-proton motive force interconversion Na,H/K antiporter P-type ATPase, alpha subunit (TIGR01106; EC 3.6.3.-; HMM-score: 92.8)
    potassium/sodium efflux P-type ATPase, fungal-type (TIGR01523; EC 3.6.3.-; HMM-score: 87.3)
    Metabolism Transport and binding proteins Cations and iron carrying compounds magnesium-translocating P-type ATPase (TIGR01524; EC 3.6.3.2; HMM-score: 82.8)
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 25.1)
    phospholipid-translocating P-type ATPase, flippase (TIGR01652; EC 3.6.3.1; HMM-score: 22.1)
    Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 20.3)
    Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 18.4)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 13.2)
  • TheSEED:  
    Potassium metabolism Potassium metabolism - no subcategory Potassium homeostasis  Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)
  • PFAM:
    HAD (CL0137) Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 109.5)
    no clan defined E1-E2_ATPase; E1-E2 ATPase (PF00122; HMM-score: 108.6)
    and 2 more
    HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 26.7)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 14.7)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001939
    • TAT(Tat/SPI): 0.000079
    • LIPO(Sec/SPII): 0.000612
  • predicted transmembrane helices (TMHMM): 7

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MHHVNKYFNQTMVIEALKMSFYKLNLKQLIKNPIMFVVEVGMILTLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQSNLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESAPVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIALFTLLTTLTIIFLVVIVTLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGMDRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAAYMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGAPNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVERFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIVAMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMTRGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIVALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]