PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL2068 [new locus tag: SACOL_RS10820 ]
• pan locus tag^?: SAUPAN005349000
• symbol: kdpA
• pan gene symbol^?: kdpA
• synonym:
• product: potassium-transporting ATPase subunit A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL2068 [new locus tag: SACOL_RS10820 ]
• symbol: kdpA
• product: potassium-transporting ATPase subunit A
• replicon: chromosome
• strand: -
• coordinates: 2131774..2133450
• length: 1677
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3238587 NCBI
• RefSeq: YP_186884 NCBI
• BioCyc: see SACOL_RS10820
• MicrobesOnline: 913545 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGAAATAATTTTGTTTCTAACAATGATGGTTATGATTACGTATGTTTTTAGTGGATAT
  CTATATCGCGTAGCATTGGTGCAATCGTCAAGAGTAGATTTAATATTTACTAGATTTGAA
  AATATGTGTTTTAAAATTATCGGCACAGATTTAGAACACATGTCAGCTAAAACATATGTC
  AAACATTTTCTGGCATTTAATGGATTTATGGGGTTTATAACATTTGTATTGCTAATAGTT
  CAGCAATGGCTTTTTTTAAATCCAAATCATAATTTAAACCAATCGATAGATTTAGCGTTT
  AATACAGCAATATCTTTTTTAACAAATAGTAATTTACAACACTATAACGGTGAATCAGAT
  GTGACATATTTAACGCAAATGATTGTAATGACATATTTAATGTTTACATCTAGTGCATCA
  GGTTACGCCGTTTGTATAGCGATGTTGAGACGTTTAACTGGATTAACTAATATCATTGGT
  AATTTTTATCAAGATATTGTTCGGTTTATTGTCCGAGTACTTTTACCATTATCATGTTTA
  ATTAGTATTTTATTGATGACTCAAGGTGTACCACAAACGTTGCATGCTAATTTAATGATT
  CGGACTTTAAGCGGACATATTCAACATATTGCATTTGGACCTATTGCATCACTTGAATCA
  ATAAAACATCTTGGTACGAATGGTGGAGGATTTTTAGCAGGAAATTCTGCAACACCTTTT
  GAAAATCCAAATATTTGGAGCAATTTTATAGAAATGGGCAGTATGATGTTACTTCCTATG
  TCAATGTTGTTCTTATTTGGTCGCATGTTAAGTAGACATGGTAAACGAGTACATCGTCAT
  GCGTTGATATTATTTGTCGCAATGTTTTTCATTTTTATAGCAATTCTTACATTAACTATG
  TGGAGTGAGTATCGTGGTAATCCAATACTAGCGAATTTAGGCATTTATGGACCGAATATG
  GAAGGTAAAGAGGTACGGTTTGGAGCAGGTTTGTCAGCACTATTTACAGTTATTACGACG
  GCATTTACAACGGGTTCTGTTAATAACATGCATGATAGCTTAACGCCTATAGGTGGATTA
  GGACCAATGGTATTAATGATGCTAAATGTTGTATTTGGTGGCGAAGGCGTAGGACTCATG
  AATTTATTGATATTTGTCTTACTGACGGTGTTTATATGCAGTTTGATGGTTGGTAAAACA
  CCAGAATATTTAAATATGCCAATTGGCGCCCGTGAAATGAAATGTATTGTCTTAGTCTTT
  CTCATACACCCAATTTTAATTTTAGTATTTTCAGCACTTGCTTTTATGATTCCTGGAGCA
  AGTGAAAGTATAACGAATCCGTCTTTTCATGGTATTTCACAAGTTATGTATGAAATGACA
  TCAGCTGCTGCGAACAATGGATCAGGGTTTGAAGGACTGAAAGATGATACAACATTCTGG
  AATATCTCTACAGGAATCATTATGTTGCTTTCTCGTTATATACCAATTATTTTGCAATTA
  ATGATTGCATCAAGTTTAGTGAATAAAAAATCATACCATCAAGATAAATATACAATAGCG
  ATTGATAAACCTTATTTTGGCGTATCACTAATCGTATTTATCGTTTTACTAAGTGGTTTG
  ACATTTATTCCAGTACTATTACTTGGTCCTATTGGTGAATTTTTAACTTTAAAATAA
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⊟Protein

⊟General

• locus tag: SACOL2068 [new locus tag: SACOL_RS10820 ]
• symbol: KdpA
• description: potassium-transporting ATPase subunit A
• length: 558
• theoretical pI: 8.44178
• theoretical MW: 62025.5
• GRAVY: 0.688351

⊟Function

• reaction:
  EC 3.6.3.12?  ExPASy
  Potassium-transporting ATPase ATP + H₂O + K⁺(Out) = ADP + phosphate + K⁺(In)
• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds K+-transporting ATPase, A subunit (TIGR00680; EC 3.6.3.12; HMM-score: 580.7)
• TheSEED  :
  • Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1)
  Potassium metabolism Potassium metabolism - no subcategory Potassium homeostasis  Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1)
• PFAM:
  Ion_channel (CL0030) KdpA; Potassium-transporting ATPase A subunit (PF03814; HMM-score: 706.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.087662
  • TAT(Tat/SPI): 0.001003
  • LIPO(Sec/SPII): 0.017459
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 57650750 NCBI
• RefSeq: YP_186884 NCBI
• UniProt: Q5HEC3 UniProt

⊟Protein sequence

• MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCFKIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNTAISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIGNFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASLESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKRVHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSALFTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFICSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFHGISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVNKKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SACOL2065 < kdpC < kdpB < kdpA < kdpF

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications