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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2034 [new locus tag: SAUSA300_RS11190 ]
  • pan locus tag?: SAUPAN005349000
  • symbol: kdpA
  • pan gene symbol?: kdpA
  • synonym:
  • product: potassium-transporting ATPase subunit A

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2034 [new locus tag: SAUSA300_RS11190 ]
  • symbol: kdpA
  • product: potassium-transporting ATPase subunit A
  • replicon: chromosome
  • strand: -
  • coordinates: 2195576..2197252
  • length: 1677
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGAAATAATTTTGTTTCTAACAATGATGGTTATGATTACGTATGTTTTTAGTGGATAT
    CTATATCGCGTAGCATTGGTGCAATCGTCAAGAGTAGATTTAATATTTACTAGATTTGAA
    AATATGTGTTTTAAAATTATCGGCACAGATTTAGAACACATGTCAGCTAAAACATATGTC
    AAACATTTTCTGGCATTTAATGGATTTATGGGGTTTATAACATTTGTATTGCTAATAGTT
    CAGCAATGGCTTTTTTTAAATCCAAATCATAATTTAAACCAATCGATAGATTTAGCGTTT
    AATACAGCAATATCTTTTTTAACAAATAGTAATTTACAACACTATAACGGTGAATCAGAT
    GTGACATATTTAACGCAAATGATTGTAATGACATATTTAATGTTTACATCTAGTGCATCA
    GGTTACGCCGTTTGTATAGCGATGTTGAGACGTTTAACTGGATTAACTAATATCATTGGT
    AATTTTTATCAAGATATTGTTCGGTTTATTGTCCGAGTACTTTTACCATTATCATGTTTA
    ATTAGTATTTTATTGATGACTCAAGGTGTACCACAAACGTTGCATGCTAATTTAATGATT
    CGGACTTTAAGCGGACATATTCAACATATTGCATTTGGACCTATTGCATCACTTGAATCA
    ATAAAACATCTTGGTACGAATGGTGGAGGATTTTTAGCAGGAAATTCTGCGACACCTTTT
    GAAAATCCAAATATTTGGAGCAATTTTATAGAAATGGGCAGTATGATGTTACTTCCTATG
    TCAATGTTGTTCTTATTTGGTCGCATGTTAAGTAGACATGGTAAACGAGTACATCGTCAT
    GCGTTGATATTATTTGTCGCAATGTTTTTCATTTTTATAGCAATTCTTACATTAACTATG
    TGGAGTGAGTATCGTGGTAATCCAATACTAGCGAATTTAGGCATTTATGGACCGAATATG
    GAAGGTAAAGAGGTACGGTTTGGAGCAGGTTTGTCAGCACTATTTACAGTTATTACGACG
    GCATTTACAACGGGTTCTGTTAATAACATGCATGATAGCTTAACGCCTATAGGTGGATTA
    GGACCAATGGTATTAATGATGCTAAATGTTGTATTTGGTGGCGAAGGCGTAGGACTCATG
    AATTTATTGATATTTGTCTTACTGACGGTGTTTATATGCAGTTTGATGGTTGGTAAAACA
    CCAGAATATTTAAATATGCCAATTGGCGCCCGTGAAATGAAATGTATTGTCTTAGTCTTT
    CTCATACACCCAATTTTAATTTTAGTATTTTCAGCACTTGCTTTTATGATTCCTGGAGCA
    AGTGAAAGTATAACGAATCCGTCTTTTCATGGTATTTCACAAGTTATGTATGAAATGACA
    TCAGCTGCTGCGAACAATGGATCAGGGTTTGAAGGACTGAAAGATGATACAACATTCTGG
    AATATCTCTACAGGAATCATTATGTTGCTTTCTCGTTATATACCAATTATTTTGCAATTA
    ATGATTGCATCAAGTTTAGTGAATAAAAAATCATACCATCAAGATAAATATACAATAGCG
    ATTGATAAACCTTATTTTGGCGTATCACTAATCGTATTTATCGTTTTACTAAGTGGTTTG
    ACATTTATTCCAGTACTATTACTTGGTCCTATTGGTGAATTTTTAACTTTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1677

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2034 [new locus tag: SAUSA300_RS11190 ]
  • symbol: KdpA
  • description: potassium-transporting ATPase subunit A
  • length: 558
  • theoretical pI: 8.44178
  • theoretical MW: 62025.5
  • GRAVY: 0.688351

Function[edit | edit source]

  • reaction:
    EC 3.6.3.12?  ExPASy
    Potassium-transporting ATPase ATP + H2O + K+(Out) = ADP + phosphate + K+(In)
  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds K+-transporting ATPase, A subunit (TIGR00680; EC 3.6.3.12; HMM-score: 580.7)
  • TheSEED  :
    • Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1)
    Potassium metabolism Potassium metabolism - no subcategory Potassium homeostasis  Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1)
  • PFAM:
    Ion_channel (CL0030) KdpA; Potassium-transporting ATPase A subunit (PF03814; HMM-score: 706.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.087662
    • TAT(Tat/SPI): 0.001003
    • LIPO(Sec/SPII): 0.017459
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCFKIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNTAISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIGNFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASLESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKRVHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSALFTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFICSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFHGISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVNKKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]