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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02401
  • pan locus tag?: SAUPAN005496000
  • symbol: SAOUHSC_02401
  • pan gene symbol?: mtlR
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02401
  • symbol: SAOUHSC_02401
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2221120..2223252
  • length: 2133
  • essential: no DEG other strains
  • comment: The sequence of SAOUHSC_02401 was corrected based on the resequencing performed by Berscheid et al., 2012 [1].

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTTATTGAGTACACGTGAAAAAGAAATGATAGCCCTATTGATTAAGTACCACGGTCAA
    TATATCACTATACACGACATTGCTCAGCAACTTGCGGTGTCCTCTCGTACTATTCACCGT
    GAATTAAAAGGTGTTGAAGCATATTTAACTTCATTTTCATTAACTTTAGAACGCGCAAAC
    AAAAAAGGGCTACGCATTGCTGGCACAGATTCTGATTTAAACGATTTGAAGCAATCGATT
    GCACAACATCAAACCATTGACTTATCTGTTGAAGAGCAGAAAGTAATTATTATATACGCT
    TTGATACAAGCCAAGGAGCCAGTTAAACAATATAGTTTAGCGCAAGAAATTGGCGTTTCT
    GTCCAGACTTTAGCAAAGATGTTAGATGATTTAGAGCTTGATTTAAATAAGTACCAACTA
    TCTTTATCTCGAAAGCGTGGCGAAGGCATTTACTTGGTAGGTACTGAATCAAAGAAACGT
    GAATTTTTAAGTCAATTAATGGTGAATAACTTAAATAGTACTAGCGTTTATTCAGTAATT
    GAAAATCATTTTGTCTTTCATTCATTAAATCAAATCCACAAAGACTTTGTTGACTTAGAG
    CGCATTTTTAATGTTGAAAGACTATTAATGGACTACCTAAGTGCCTTACCCTACCAACTT
    ACCGAATCAAGTTATTTAACTTTAACTGTCCATATCGTGCTCTCCATTTCACGTATAAAA
    AATGGAGAGTATGTCGCATTAAACGATGATATTTATGATTCTGTACAAAACACATTTGAA
    CACAAAGTAGCAAGCGAACTTGCTGATAAACTTGGTCAAATATATGACGTCACGTTTAAT
    CAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAAT
    GATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTA
    GAAATGATTTCCGGTATGACATTTGCAGATTTGGATACTTTAGTAGATGGACTGACGCTA
    CACCTTAATCCTGCAATCAATCGTTTGCAAGCTAATATCGAGACCTATAATCCGTTAACA
    GACATGATTAAGTTCAAATATCCAAGACTATTTGAAAATGTAAGATTAGCTTTAAATGAT
    TGTTGGCCTGATTTGATTTTTCCAGAGAGTGAAATTGCTTTTATAGTTTTACACTTTGGT
    GGCTCGATTAAAAACCAAGGTAATCGATTTTTAAACATATTAGTCGTTTGCAGCAGTGGT
    ATGGGAACTAGTCGTCTATTATCAACTCGTCTAGAGCAAGTTTTTAGTGAGATTGAGCGT
    ATTACACAAGCATCAGTCAGCGATTTGAAGTCACTAGATTTAAGTCAATATGATGGCATT
    ATTTCTACTGTGAATTTAGACATCGACTCCCCCTATTTAACGGTAAACCCATTATTACCA
    GATAGTGATATCAGTTATGTCGCACAGTTTTTAAATACAAAGTCTACGTTCCAAGAGACG
    CATGATAAATCATCAAACATGATTGATAAGGATGATGTTCATGTTGAAACGAAAGATGTT
    GATGGCAACACATCTTTTGAAAATGAACAAACTTCATACTTAACTTCAGTTTTCGAAAAA
    CATTTAAGTGACGAAAAATCAGAACAATTATTGCATCATATGCGTTCGGGTTTAACTTTG
    CTTGATTCAGTGAAAATAGTTAGTACCGAAGTTAAACAGTGGCAAACATATATCGCAGAT
    TATCTATATCAATGCGATGTAATAAACGATCCAACGTCATTCGCTGAACTACTAGAGCAA
    CGATTGATTGACAATCCAGGATGGATATTAAGTCCATATCCTGTTGCAATACCACACCTA
    AGAGACAATATGATTAAACACCCTATGATTCTAATCACAGTTTTAGAAGAACCGTTAACA
    TTGCCTAGTATTCAAAATGACAATCAAACAATTAAATATATGATTTCCATGTTTATTTCT
    GACAATGATTTTATGGCATCACTGGTAAGTGACTTGTCCGAATTTTTAAGTTTGAAATTA
    GAATCTATTGATACTTTTATGGAAAATCCACAGGAACTTGAAACATTATTAAGAAACAAA
    TTTTTAGAACGAATTAAAAAACAATTTATTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2133

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02401
  • symbol: SAOUHSC_02401
  • description: hypothetical protein
  • length: 710
  • theoretical pI: 4.88526
  • theoretical MW: 81145.1
  • GRAVY: -0.175493

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 28)
    Signal transduction Signal transduction PTS PTS system, fructose subfamily, IIA component (TIGR00848; EC 2.7.1.69; HMM-score: 28)
    and 9 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 16.3)
    Signal transduction Regulatory functions DNA interactions iron-sulfur cluster biosynthesis transcriptional regulator SufR (TIGR02702; HMM-score: 16.3)
    RNA polymerase sigma-70 factor, sigma-B/F/G subfamily (TIGR02980; HMM-score: 16)
    RNA polymerase sigma factor, TIGR02999 family (TIGR02999; HMM-score: 13.6)
    RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 13)
    Unknown function Enzymes of unknown specificity NDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 12.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 12.7)
    Signal transduction Signal transduction PTS PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 12.7)
    probable regulatory domain (TIGR03879; HMM-score: 12.6)
  • TheSEED  :
    • Mannitol operon activator, BglG family
    Carbohydrates Sugar alcohols Mannitol Utilization  Mannitol operon activator, BglG family
  • PFAM:
    PRD (CL0166) PRD; PRD domain (PF00874; HMM-score: 118.2)
    and 21 more
    HTH (CL0123) Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 56.7)
    HTH_11; HTH domain (PF08279; HMM-score: 50.1)
    PTase-anion_tr (CL0340) PTS_EIIA_2; Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2 (PF00359; HMM-score: 34.4)
    Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 29.1)
    HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 21.1)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 20.5)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 18.4)
    IF2_N; Translation initiation factor IF-2, N-terminal region (PF04760; HMM-score: 18)
    HTH_10; HTH DNA binding domain (PF04967; HMM-score: 16.9)
    DUF2089; Protein of unknown function (DUF2089) (PF09862; HMM-score: 16.9)
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 16.4)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 15.9)
    MarR; MarR family (PF01047; HMM-score: 15.6)
    HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 14.9)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 14.9)
    HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 13.5)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 13.4)
    no clan defined DUF746; Domain of Unknown Function (DUF746) (PF05344; HMM-score: 13.1)
    DUF2675; Protein of unknown function (DUF2675) (PF11247; HMM-score: 13)
    HTH (CL0123) SMC_ScpB; Segregation and condensation complex subunit ScpB (PF04079; HMM-score: 12.5)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors: HPr (phosphocarrier protein); MtlA (mannitol-specific enzyme IICB PTS component)
  • genes regulated by MtlR*, TF important in Mannitol utilizationRegPrecise
    activation
    mtlA* > mtlR* > mtlF* > mtlD*
    transcription unit transferred from N315 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003666
    • TAT(Tat/SPI): 0.00113
    • LIPO(Sec/SPII): 0.000625
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKGVEAYLTSFSLTLERANKKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALIQAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKREFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPYQLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYDVTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTLVDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEIAFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLKSLDLSQYDGIISTVNLDIDSPYLTVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMIDKDDVHVETKDVDGNTSFENEQTSYLTSVFEKHLSDEKSEQLLHHMRSGLTLLDSVKIVSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNMIKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFISDNDFMASLVSDLSEFLSLKLESIDTFMENPQELETLLRNKFLERIKKQFI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: SigB* (activation) regulon, MtlR* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [5] [4]   other strains
    MtlR*(TF)important in Mannitol utilization; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Anne Berscheid, Peter Sass, Konstantin Weber-Lassalle, Ambrose L Cheung, Gabriele Bierbaum
    Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.
    Int J Med Microbiol: 2012, 302(2);84-7
    [PubMed:22417616] [WorldCat.org] [DOI] (I p)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  5. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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