From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02750
  • pan locus tag?: SAUPAN006006000
  • symbol: SAOUHSC_02750
  • pan gene symbol?: aimA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02750
  • symbol: SAOUHSC_02750
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2525682..2527319
  • length: 1638
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATAAAGACAAGCAATTGCACAACGACAAAATCAATCTATCCCAATTAGTCTTATTA
    GGGTTAGGCTCTTTAATAGGATCTGGTTGGCTATTTGGTGCGTGGGAAGCATCATCAATA
    GCTGGACCAGCAGCAATCATATCATGGGTTCTTGGATTCCTAGTCATTGGAACCATTGCC
    TATAACTACATTGAAATCGGCACAATGTTTCCTCAATCAGGTGGCATGAGTAACTATGCC
    CAGTATACACATGGCTCATTATTAGGCTTTATTGCTGCTTGGGCGAATTGGGTGTCTTTG
    GTGACAATAATACCTATCGAAGCTGTGTCAGCTGTTCAATATATGAGTTCTTGGCCGTGG
    CATTGGGCGAAACCAATGAGATATTTAATGGAAAATGGCTCTATTAGCACATACGGATTG
    CTAGCTGTATATCTCATCATTGTTATTTTTTCATTATTAAACTATTGGTCCGTAAAACTT
    TTAACATCATTTACGAGTTTAATTTCTGTATTTAAATTAGGCGTACCCATGTTAACCATC
    ATCATGTTGATGCTATCAGGATTCGACACTTCAAATTACGGCCATTCGGCAAGCACATTT
    ATGCCTTACGGAAGTGCACCGATTTTTGCTGCAACAACAGCATCAGGGATTATTTTTTCA
    TTCAATTCATTCCAGACAATTATTAATATGGGTTCAGAAATTAAAAATCCTGAAAAAAAT
    ATCGCAAGAGGCATCGCTATCTCACTGTCAATCAGTGCAGTGTTGTACATCATTTTACAA
    AGTACGTTTATCACTTCTATGCCTCAATCAATGTTACAACATAGTGGATGGAATGGCATC
    AACTTCAATTCACCATTTGCTGATTTAGCTATCTTATTAGGAATTAATTGGCTCGCAATT
    TTACTATACATTGAAGCTTTTGTATCACCATTCGGTACTGGCGTGTCATTTGTCGCCGTT
    ACAGGTCGAGTTTTACGAGCAATGGAGAAAAATGGACATATCCCTAAATTTCTTGGGAAG
    ATGAATGAAAAATATCATATCCCACGTGTAGCAATCATCTTTAATGCCATCATTAGTATG
    ATTATGGTTACATTATTTAGAGATTGGGGTACGCTAGCAGCAGTTATTTCTACTGCAACT
    TTAGTAGCCTATTTAACTGGCCCAACGACAGTGATTGCATTAAGAAAAATGGGACCAACA
    ATGACTCGTCCATTTAGAGCAAAAATTTTAAAAGTAATGGCACCATTATCATTTGTATTA
    GCTTCATTAGCTATATATTGGGCAATGTGGCCAACAACGGCTGAAGTTATTTTAATCATT
    ATACTTGGATTACCAATCTACTTCTTCTATGAATATCGTATGAATTGGCGTAATACAAAG
    AAACAAATTGGTGGTAGCTTATGGATTATTGTATATTTAATCGTGCTATCAATACTGTCA
    TTTATAGGAAGCAAAGAATTTAAAGGCTTAAATATGATTCACTATCCATTTGACTTTATC
    GTTATTATTATTGTGGCACTTATCTTCTATTACATCGGTACAACGAGTTCATTTGAAAGC
    GTCTATTTCCGTCGCGCAACACGAATCAATACGAAGATGCGTGAGTCACTAAATAATGAA
    TCAAAGTCATCTCATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1638

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02750
  • symbol: SAOUHSC_02750
  • description: hypothetical protein
  • length: 545
  • theoretical pI: 10.0252
  • theoretical MW: 60640.3
  • GRAVY: 0.604404

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 175.7)
    and 10 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 98.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines L-type amino acid transporter (TIGR00911; HMM-score: 93.6)
    Metabolism Transport and binding proteins Amino acids, peptides and amines transporter, basic amino acid/polyamine antiporter (APA) family (TIGR00905; HMM-score: 91.6)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 70.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 56.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 50.3)
    histidine-histamine antiporter (TIGR04298; HMM-score: 46.8)
    putrescine-ornithine antiporter (TIGR04299; HMM-score: 33.1)
    putative glutamate/gamma-aminobutyrate antiporter (TIGR03813; HMM-score: 31.3)
    Metabolism Transport and binding proteins Amino acids, peptides and amines arginine-ornithine antiporter (TIGR03810; HMM-score: 30.2)
  • TheSEED  :
    • Amino acid transporter
  • PFAM:
    APC (CL0062) AA_permease_2; Amino acid permease (PF13520; HMM-score: 145.8)
    and 2 more
    AA_permease; Amino acid permease (PF00324; HMM-score: 108.6)
    no clan defined Ac76; Orf76 (Ac76) (PF05814; HMM-score: 20.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.053846
    • TAT(Tat/SPI): 0.005643
    • LIPO(Sec/SPII): 0.055996
  • predicted transmembrane helices (TMHMM): 14

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPAAIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVTIIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKLLTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGIIFSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSGWNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHIPKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVIALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFYEYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALIFYYIGTTSSFESVYFRRATRINTKMRESLNNESKSSH

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_02750&oldid=70231"