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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02765
  • pan locus tag?: SAUPAN006025000
  • symbol: SAOUHSC_02765
  • pan gene symbol?: cntC
  • synonym:
  • product: nickel ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02765
  • symbol: SAOUHSC_02765
  • product: nickel ABC transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 2541043..2541912
  • length: 870
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGATAATTTTAAAACGATTATTACAAGATAAAGGTGCAGTAATTGCTTTAGGCATTATT
    GTATTATATGTCTTTTTAGGATTAGCAGCACCACTTGTGACATTTTATGATCCTAACCAT
    ATCGATACAGCAAACAAATTTGCTGGCATGAGTTTTCAACATCTACTAGGTACTGACCAT
    TTAGGTAGAGATATTTTAACTAGGTTAATTTATGCGATTAGACCAAGTTTGTTATATGTC
    TTTGTTGCGCTATTTGTTTCTGTACTTATTGGATCTATTTTAGGATTCTTATCAGGATAT
    TTCCAAGGGTTTGTTGACGCCTTAATCATGCGTGCGTGTGATGTTATGTTGGCATTCCCA
    AGTTATGTTGTAACGTTAGCATTAATTGCATTGTTTGGAATGGGTGCCGAAAATATTATC
    ATGGCATTTATTTTGACGCGTTGGGCATGGTTCTGTCGTGTTATACGTACAAGTGTTATG
    CAGTACACTGCTTCTGACCATGTAAGATTTGCTAAAACAATCGGTATGAATGATATGAAA
    ATTATTCACAAACATATTATGCCATTAACATTAGCAGATATTGCTATCATCTCTAGTAGC
    TCGATGTGTTCAATGATCTTGCAAATATCTGGCTTTTCATTTTTAGGATTAGGTGTCAAA
    GCGCCTACTGCAGAGTGGGGCATGATGCTTAACGAAGCTAGAAAAGTGATGTTTACACAT
    CCTGAAATGATGTTTGCGCCAGGTATTGCCATAGTGATTATAGTGATGGCATTTAACTTC
    TTATCCGATGCTTTACAAATTGCTATTGATCCCCGCATCTCTTCTAAAGATAAACTTCGT
    TCTGTGAAAAAAGGAGTGGTGCAATCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    870

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02765
  • symbol: SAOUHSC_02765
  • description: nickel ABC transporter permease
  • length: 289
  • theoretical pI: 9.76066
  • theoretical MW: 31965.3
  • GRAVY: 0.802076

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 284.3)
    and 3 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 29.4)
    2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 18.1)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 14.4)
  • TheSEED  :
    • Staphylopine-metal ABC transporter, permease protein CntC
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 88.1)
    and 2 more
    no clan defined OppC_N; N-terminal TM domain of oligopeptide transport permease C (PF12911; HMM-score: 25.2)
    CM2; Influenza C virus M2 protein (PF03021; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.084455
    • TAT(Tat/SPI): 0.001713
    • LIPO(Sec/SPII): 0.007846
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIILKRLLQDKGAVIALGIIVLYVFLGLAAPLVTFYDPNHIDTANKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQGFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVMQYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLGVKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSKDKLRSVKKGVVQS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Laetitia Remy, Marie Carrière, Aurélie Derré-Bobillot, Cécilia Martini, Maurizio Sanguinetti, Elise Borezée-Durant
The Staphylococcus aureus Opp1 ABC transporter imports nickel and cobalt in zinc-depleted conditions and contributes to virulence.
Mol Microbiol: 2013, 87(4);730-43
[PubMed:23279021] [WorldCat.org] [DOI] (I p)

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