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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02978
  • pan locus tag?: SAUPAN006374000
  • symbol: SAOUHSC_02978
  • pan gene symbol?: —
  • synonym:
  • product: phage infection protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02978
  • symbol: SAOUHSC_02978
  • product: phage infection protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2741077..2744058
  • length: 2982
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGAACGCATTTAAATTATTTAAAATGGATCTGAAGAAAGTAGCTAAGACGCCAGCT
    GTGTGGATTATCTTAGCAGGCTTAGCTATTTTGCCATCGTTCTACGCTTGGTTTAACTTA
    TGGGCAATGTGGGATCCATATGGCAACACGGGACACATCAAGGTCGCAGTCGTTAATGAA
    GATAAAGGCGACACAATCAGAGGGAAAAAAGTTAATGTCGGTAATACGATGGTTAATACA
    CTCAAGAAAAATAAAAGTTTTGATTGGCAGTTTGTAAGTAGAGAGAAAGCTGATCATGAG
    ATAAAAATGGGTAAATATTTTGCAGGTATTTACATCCCATCTAAGTTTACACATGAAATT
    ACAGGGACACTACGTAAGCAGCCTCAAAAAGCAGATGTAGAATTTAAGGTGAATCAGAAG
    ATTAACGCTGTTGCGTCTAAGCTAACAGATACTGGTTCGTCAGTTGTCGTTGAAAAAGCG
    AATGAACAATTTAATAAAACAGTAACTCGAGCATTATTAGAAGAAGCTAACAAAGCAGGT
    TTAACTATTGAAGAAAATGTGCCGACAATTAACAAGATAAAAAATGCGGTATATTCAGCA
    GATAAAGCTTTACCTAAGATTAATGACTTTGCGAATAAAATTGTATATTTGAATAACCAC
    CAAGCGGATTTAGATAAATATGCCAATGATTTTAGAAAACTAGGAAATTATAAAGGTGAT
    ATTTTAGATGCTCAGAAAAAATTAAACGAAGTCAATGGTGCTATTCCGCAACTTAATGAA
    AAGGCTAAGTTGATATTAGCTTTAAATAATTATATGCCGAAAATTGAAAAAGCGTTAAAT
    TTTGCAGCTGATGACGTGCCAGCGCAGTTCCCTAAAATTAATCAAGGACTTAACATTGCG
    AGTCAAGGTATTGATCAAGCTAATGGACAGTTAAATGATGCCAAAGGCTTCGTCACACAA
    GTTAGAAGTAGAGTCGGTGATTATCAAGATGCAATTCGACGCGCGCAAGATTTAAATCGA
    AGAAACCAGCAACAGATTCCTCAAAATAGCGCGGCGAACAACGAAACATCAAATAGTGCA
    CCTGCAGCTGGTAATGGTGTAGCATCAACGCCACCAAGTGCACCAAGTGGCGATACTGCA
    CCAAATAATAATGTTACGCAAAATACCGCACCAAATAGTAATAATGCGCCTGTATCGACT
    ACACCACAAAGTACAAGCGGGAAAAAAGATGGTCAAAGTTTTGTAGATATAACAACAACA
    CAAGTCAGCACAGCTAACGAGAACACACAAAACATTACAGATAAAGATGTTAAATCAATG
    GAAGCGGCATTAACGGGCTCTTTATTATCATTATCAAATAATTTAGATACCCAAGCGAAA
    GCCGCACAAAAAGATAGTCAGGCATTACGTAATATTTCGTATGGGATTTTAGCATCGGAC
    AAGCCTTCTGATTTTAGAGAGTCTTTAGATAATGTTAAGTCCGGTTTAGAATACACAACG
    CAATATAATCAACAATTTATCGATACATTAAAAGAGATTGAGAAGAATGAAAATGTTGAT
    TTATCAAAAGAAATTGATAAGGTAAAAGCAGCTAATAATCGAATTAATGAATCATTAAGG
    TTAGTTAATCAATTAAGCAATGCATTAAAGAATGGTAGTTCAGGAACTGCTGAAGCTACT
    AAATTACTAGATCAACTTTCAAAACTAGATTCATCATTATCATCATTTAGAGATTATGTT
    AAAAAAGATCTTAACAGCTCTTTAGTATCAATATCACAACGTATTATGGATGAATTGAAC
    AAAGGGCAAACTGCATTATCCAATGTTCAGTCTAAATTAAATACAATTGATCAAGTCATT
    AACAGTGGACAAGCTATTTTAAAAAATGGTAAAACACGTATTGATCGCTTACAAACAGTA
    TTACCAAGTATTGAACAACAATACATTAGTGCTGTTAAAAATGCTCAAGCAAACTTCTCG
    AAAGTGAAAAGTGATGTAGCTAAAGCTGCTAACTTTGTGCGCAATGACTTACCACAGTTA
    GAACAGCGATTAACTAATGCGACAGCAAGTGTGAATAAAAATTTACCAACGTTATTAAAT
    GGTTATGATCAAGCGGTAGGATTACTAAATAAAAATCAGCCACAAGCGAAAAAGGCTTTA
    TCAGATTTAGCTGATTTTTCTCAAAATAAATTGCCTGATGTTGAAAAAGATTTGAAAAAA
    GCGAATAAAATTTTCAAGAAATTAGACAAAGATGATGCAGTCGACAAATTAATCGACACA
    CTTAAGAATGATTTGAAAAAGCAAGCGGGTATTATTGCAAATCCTATTAATAAGAAGACT
    GTTGATGTTTTCCCAGTTAAGGATTATGGTTCAGGTATGACACCATTCTATACTGCACTG
    TCAGTATGGGTAGGTGCACTCTTGATGGTAAGTTTATTAACGGTTGATAATAAACATAAG
    AGTCTAGAGTCAGTCTTAACGACAAGACAAGTGTTCTTAGGTAAGGCAGGATTCTTTATA
    ATGCTTGGTATGTTGCAAGCACTCATTGTATCGGTTGGAGATTTGTTAATCCTAAAAGCA
    GGAGTTGAGTCACCTGTATTATTTGTACTTATAACGATTTTCTGTTCGATTATTTTCAAC
    TCAATCGTATATACGTGCGTATCATTACTTGGTAACCCAGGTAAAGCCATTGCAATCGTA
    TTGCTTGTATTACAAATTGCAGGTGGTGGGGGAACATTCCCAATTCAAACTACGCCACAA
    TTTTTCCAAAACATTTCGCCATACTTACCATTTACGTATGCAATTGATTCATTACGTGAA
    ACAGTAGGCGGTATTGTTCCGGAAATCCTAATTACAAAATTAATTATATTAACGTTATTT
    GGTATAGGATTCTTCGTTGTAGGTTTAATTTTAAAACCTGTAACAGATCCATTGATGAAG
    CGCGTATCTGAAAAAGTTGACCAAAGTAACGTTACAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    2982

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02978
  • symbol: SAOUHSC_02978
  • description: phage infection protein
  • length: 993
  • theoretical pI: 9.90943
  • theoretical MW: 108715
  • GRAVY: -0.279255

⊟Function[edit | edit source]

  • TIGRFAM:
    YhgE/Pip C-terminal domain (TIGR03062; HMM-score: 167.6)
    YhgE/Pip N-terminal domain (TIGR03061; HMM-score: 158.5)
    and 3 more
    type VII secretion protein EsaA (TIGR03929; HMM-score: 83.4)
    Metabolism Transport and binding proteins Other daunorubicin resistance ABC transporter membrane protein (TIGR01247; HMM-score: 20.7)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking twin arginine-targeting protein translocase, TatA/E family (TIGR01411; HMM-score: 6.2)
  • TheSEED  :
    • Phage infection protein
  • PFAM:
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 86.6)
    and 14 more
    ABC2_membrane; ABC-2 type transporter (PF01061; HMM-score: 31.3)
    DUF3533; Protein of unknown function (DUF3533) (PF12051; HMM-score: 22.6)
    no clan defined ADIP; Afadin- and alpha -actinin-Binding (PF11559; HMM-score: 18.9)
    Baculo_PEP_C; Baculovirus polyhedron envelope protein, PEP, C terminus (PF04513; HMM-score: 15.8)
    DUF842; Eukaryotic protein of unknown function (DUF842) (PF05811; HMM-score: 13.9)
    Globin (CL0090) HisK-N-like; HisK-N-like globin domain of the ASK signalosome (PF20302; HMM-score: 11.6)
    SNARE-fusion (CL0445) V-SNARE_C; Snare region anchored in the vesicle membrane C-terminus (PF12352; HMM-score: 10.8)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 9.3)
    SNARE-fusion (CL0445) Sec20; Sec20 (PF03908; HMM-score: 8.1)
    no clan defined DUF2935; Domain of unknown function (DUF2935) (PF11155; HMM-score: 7.9)
    BBS2_hp; BBS2 hairpin (PF23353; HMM-score: 7.5)
    DUF2203; Uncharacterized conserved protein (DUF2203) (PF09969; HMM-score: 7.4)
    EsxAB (CL0352) Hydro_N_hd; Hydrolase N-terminal helical domain (PF22905; HMM-score: 7.1)
    no clan defined Prominin; Prominin (PF05478; HMM-score: 6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0.01
    • Extracellular Score: 0.01
    • Internal Helices: 6
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0002
    • Cytoplasmic Membrane Score: 0.9854
    • Cell wall & surface Score: 0.0085
    • Extracellular Score: 0.0059
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.069564
    • TAT(Tat/SPI): 0.001126
    • LIPO(Sec/SPII): 0.006006
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMWDPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIKMGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKANEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLNNHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIEKALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKGFVTQVRSRVGDYQDAIRRAQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSAPSGDTAPNNNVTQNTAPNSNNAPVSTTPQSTSGKKDGQSFVDITTTQVSTANENTQNITDKDVKSMEAALTGSLLSLSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFIDTLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQLSKLDSSLSSFRDYVKKDLNSSLVSISQRIMDELNKGQTALSNVQSKLNTIDQVINSGQAILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFSKVKSDVAKAANFVRNDLPQLEQRLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKKANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYTALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLLILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFPIQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILKPVTDPLMKRVSEKVDQSNVTE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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