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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
⊟Summary[edit | edit source]
- pan ID?: SAUPAN000499000
- symbol?: —
- synonym:
- description?: hypothetical protein
- strand?: -
- coordinates?: 538877..539167
- synteny block?: BlockID0002280
- occurrence?: in 15% of 34 strains
ssbmec : cassette chromosome IVa ssDNA-binding protein [1]
• A crystal structure is available : 6BTC
Contrary to initial assessment, staphylococcal cassette chromosomes are replicative following excision. The strategy employed by Cch1 consists of a minimal four-protein replicative core of ssbmec-cch1-ccrA-ccrB. In this schema, cch1 functions as a self-loading helicase that assembles as a pair of hexamers and unwinds the dsDNA in a 3'→5' orientation with ssbmec protecting the unwound ssDNA and preventing re-annealing. There is considerable sequence diversity in the ssbmec; this version comes from cassette chromosome IVa.
⊟Orthologs[edit | edit source]
COL:
—
N315:
—
NCTC8325:
—
04-02981:
—
08BA02176:
—
11819-97:
—
6850:
—
71193:
—
ECT-R 2:
—
ED133:
—
ED98:
—
HO 5096 0412:
—
JH1:
—
JH9:
—
JKD6008:
—
JKD6159:
SAA6159_00041
JSNZ:
—
LGA251:
—
M013:
—
MRSA252:
—
MSHR1132:
SAMSHR1132_00430
MSSA476:
—
Mu3:
—
Mu50:
—
MW2:
MW0041
RF122:
—
ST398:
—
T0131:
—
TCH60:
—
TW20:
—
USA300_TCH1516:
USA300HOU_0042
VC40:
—
⊟Genome Viewer[edit | edit source]
⊟Alignments[edit | edit source]
- alignment of orthologues:
- ↑ Ignacio Mir-Sanchis, Christina A Roman, Agnieszka Misiura, Ying Z Pigli, Susan Boyle-Vavra, Phoebe A Rice
Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative.
Nat Struct Mol Biol: 2016, 23(10);891-898
[PubMed:27571176] [WorldCat.org] [DOI] (I p)Ignacio Mir-Sanchis, Ying Zhang Pigli, Phoebe Ann Rice
Crystal Structure of an Unusual Single-Stranded DNA-Binding Protein Encoded by Staphylococcal Cassette Chromosome Elements.
Structure: 2018, 26(8);1144-1150.e3
[PubMed:30017563] [WorldCat.org] [DOI] (I p)