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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

Summary[edit | edit source]

  • pan ID?: SAUPAN000499000
  • symbol?:
  • synonym:
  • description?: hypothetical protein
  • strand?: -
  • coordinates?: 538877..539167
  • synteny block?: BlockID0002280
  • occurrence?: in 15% of 34 strains

ssbmec : cassette chromosome IVa ssDNA-binding protein [1]

• A crystal structure is available : 6BTC

Contrary to initial assessment, staphylococcal cassette chromosomes are replicative following excision. The strategy employed by Cch1 consists of a minimal four-protein replicative core of ssbmec-cch1-ccrA-ccrB. In this schema, cch1 functions as a self-loading helicase that assembles as a pair of hexamers and unwinds the dsDNA in a 3'→5' orientation with ssbmec protecting the unwound ssDNA and preventing re-annealing. There is considerable sequence diversity in the ssbmec; this version comes from cassette chromosome IVa.

Orthologs[edit | edit source]

    COL:
    N315:
    NCTC8325:
    Newman:
    USA300_FPR3757:
    04-02981:
    08BA02176:
    11819-97:
    6850:
    71193:
    ECT-R 2:
    ED133:
    ED98:
    HO 5096 0412:
    JH1:
    JH9:
    JKD6008:
    JKD6159:
    SAA6159_00041
    JSNZ:
    LGA251:
    M013:
    MRSA252:
    MSHR1132:
    SAMSHR1132_00430
    MSSA476:
    Mu3:
    Mu50:
    MW2:
    MW0041
    RF122:
    ST398:
    T0131:
    TCH60:
    TW20:
    USA300_TCH1516:
    USA300HOU_0042
    VC40:

Genome Viewer[edit | edit source]

Alignments[edit | edit source]

  • alignment of orthologues:

  1. Ignacio Mir-Sanchis, Christina A Roman, Agnieszka Misiura, Ying Z Pigli, Susan Boyle-Vavra, Phoebe A Rice
    Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative.
    Nat Struct Mol Biol: 2016, 23(10);891-898
    [PubMed:27571176] [WorldCat.org] [DOI] (I p)
    Ignacio Mir-Sanchis, Ying Zhang Pigli, Phoebe Ann Rice
    Crystal Structure of an Unusual Single-Stranded DNA-Binding Protein Encoded by Staphylococcal Cassette Chromosome Elements.
    Structure: 2018, 26(8);1144-1150.e3
    [PubMed:30017563] [WorldCat.org] [DOI] (I p)