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⊟Summary[edit | edit source]
- pan ID?: SAUPAN000446000
- symbol?: —
- synonym:
- description?: membrane protein
- strand?: -
- coordinates?: 454715..456508
- synteny block?: BlockID0001950
- occurrence?: in 59% of 34 strains
cch1 : cassette chromosome self-loading replicative helicase [1]
• A crystal structure is available : 5DGK
Contrary to initial assessment, staphylococcal cassette chromosomes are replicative following excision. The strategy employed by Cch1 consists of a minimal four-protein replicative core of ssbmec-cch1-ccrA-ccrB. In this schema, cch1 functions as a self-loading helicase that assembles as a pair of hexamers and unwinds the dsDNA in a 3'→5' orientation with ssbmec protecting the unwound ssDNA and preventing re-annealing.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0063
08BA02176:
—
11819-97:
MS7_0071
6850:
—
71193:
—
ECT-R 2:
—
ED133:
—
ED98:
—
HO 5096 0412:
SAEMRSA15_00380
JH1:
SaurJH1_0051
JH9:
SaurJH9_0050
JKD6008:
SAA6008_00060
JKD6159:
SAA6159_00040
JSNZ:
—
LGA251:
SARLGA251_00390
M013:
—
MRSA252:
SAR0061
MSHR1132:
—
MSSA476:
SAS0034
Mu3:
SAHV_0062
Mu50:
SAV0063
MW2:
MW0040
RF122:
—
ST398:
—
T0131:
SAT0131_00059 (yycG)
TCH60:
HMPREF0772_10426
TW20:
SATW20_00940
USA300_TCH1516:
USA300HOU_0041
VC40:
—
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MSHHD-IFRLGLYHLDTMGWHKTIPPKKDGDEKKVVTMSSCMAVEAKFWHVLENTEKIII
N315 MTQTNPSFNPSPRYKSKKGWYKDKPPKEKGGMPTEVEIAGPIVIENKFIDPKTNTEKVII
USA300_FPR3757 MSYT--LFEIGPFALDSFGWKETIPPKKDGDSEKVVRMASPIIVNARFSDPITGVEKLII
*: *. . .. ** : ***:.*. . * ::. : :: :* . ..**:**
COL SNGKDKEHTFDSDILTTRKLPSLIKYGHSINENYIKDLSYALQLMRDEMPTSDLYDGSGI
N315 TDEDQKEIVESSDILTTQKLPSLMKYGFSINEKYTKDLGYALQQMRNQLPISYLYEGVGI
USA300_FPR3757 TNNNGKKDIFESDILTTRNLPSLIKYGYSINEKYIRSLSYALQLMRDRLPLSELYEGVGI
:: . *: .******::****:***.****:* :.*.**** **:.:* * **:* **
COL IETPHGYIVMIDTIYTSKQYDQLSSLDPIVDSAYPLEPKGTFKDWLNMYINEVKGHLLLE
N315 LETPFGPIVSLSEIYTTKEFDNKSPSDAICDNAYDLTPKGTFDNWFNMYLKEVKGSLLLE
USA300_FPR3757 LETPFGYLISLDKVLKSIQFNQSSPSYPIVDSAYDLTPKGTFDNWFNMYIDEVKGHLLLE
:***.* :: :. : .: :::: *. .* *.** * *****.:*:***:.**** ****
COL LAVVFGISALVTSFLKYKREIEFAGIIFSFTGQSSTGKSTAAALAVSVAGNPTKGNQTLF
N315 LAVVFGISALVTSFLKHKHETEFAGIIFSFTGQSSTGKSTAASLAVSVAGNPTKGNETLF
USA300_FPR3757 LAVIFGISALVTSFLKHKHEIEFAGILFSFTGQSSTGKSTAAALAVSVAGNPTKGNETLF
***:************:*:* *****:***************:*************:***
COL RSWNATRNALESYLSNNFGIPIVFDELSSATFKDTTGLLYSIAEGQGRQRSNVHGEVKTP
N315 RNWNATRNALEGYLSNNFGIPIVFDELSSATFRDTTGLLYSITEGQGRQRSNVHGEVKTP
USA300_FPR3757 RSWNATRNALEGYLSNNYGIPIVFDELSSTTLRDTTGLLYSIAEGQGRQRSNVHGEVKTP
*.*********.*****:***********:*::*********:*****************
COL KNWGTSVISTSEYSIFTDSAQNDGLRVRTIEINEQFTTNATNADNIKKAVALNHGHVLPL
N315 KNWGTSVISTSEHSIFNDSAQNDGLRVRTIEINEQFTTNATNADNIKKAVALNYGHVLPL
USA300_FPR3757 KNWGTSVISTSEYSIFNDSAQNDGLRVRTIEINEQFTTNATNADNIKKAVALNYGHVLPL
************:***.************************************:******
COL VAQYLINREDEVIQWFYKEVDWFEAKLNSETNNTGIRMFKRYAVITTSAKILGRVLATDI
N315 VAKYLINREDEVIQWFYKEVDWFEAKLKDDKSNTGNRMFKRYAVITTSAKILGRVLATDI
USA300_FPR3757 VAKYLINREDEVIQWFYKEVDWFEAKLKDETNNTGIRMFKRYAVITTSAKILGRVLSTDI
**:************************:.:..*** ********************:***
COL DIAKIRDYFIDYHAHTVSERSLADKAIEVITQFVAQNRGKFSDDTALKNMFENYGLIALK
N315 DIAKIRDYFIDYHGHTISERSLADKAIDVIIQFVAQNRGKFSDEGALKNMFENYGLISLK
USA300_FPR3757 DIANIRDYFIDYHTHTVSERSLADKAIDVIIQFVAQNRGKFSDEGALKNMFENYGLISLK
***:********* **:**********:** ************: ************:**
COL DNHIEVKIIASVFKNMLLEHHFQDVNNVVNALRDKGFIESDHDRITTKRTVKDGNGKKQS
N315 DDHIEVKMIANVFKQMLNNHQFQDVNNVVNALRDKGFILADRGRQTTKRSVKDNSGKKQS
USA300_FPR3757 DDHIEVKIIANVFKQMLNNHQFQDVNNVVNALRDKGFILADRGRQTTKRSVKDNSGKKQS
*:*****:**.***:** :*:***************** :*:.* ****:***..*****
COL LVFYHLKLDSEYASIFGLTKDAEPIKAEINLDNKKILESWKKQNDDLI------------
N315 LVFYHLKLDVEFASILGLTKD------------KSLIQNWTPANDNKAAKELFKSANEGI
USA300_FPR3757 LVFYHLKLDVEFASILGLTKD------------KSLLQNWTPSNDNKAAKELFKSANEGI
********* *:***:***** *.:::.*. **:
COL -------DF
N315 GPSGVHEDF
USA300_FPR3757 GPSGVHEDF
**
- ↑ Ignacio Mir-Sanchis, Christina A Roman, Agnieszka Misiura, Ying Z Pigli, Susan Boyle-Vavra, Phoebe A Rice
Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative.
Nat Struct Mol Biol: 2016, 23(10);891-898
[PubMed:27571176] [WorldCat.org] [DOI] (I p)