PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL0043 [new locus tag: SACOL_RS00230 ]
• pan locus tag^?: SAUPAN000446000
• symbol: SACOL0043
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL0043 [new locus tag: SACOL_RS00230 ]
• symbol: SACOL0043
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 49535..51304
• length: 1770
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3236995 NCBI
• RefSeq: YP_184953 NCBI
• BioCyc: see SACOL_RS00230
• MicrobesOnline: 911527 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAGTCATCATGACATTTTTAGACTAGGCCTATACCATTTAGATACAATGGGTTGGCAT
  AAAACTATCCCACCTAAAAAAGATGGAGATGAGAAAAAAGTAGTGACTATGTCTAGTTGT
  ATGGCTGTTGAAGCTAAATTTTGGCATGTCTTAGAAAATACAGAAAAAATAATTATTTCT
  AACGGTAAGGATAAAGAACATACATTTGACTCTGATATTTTAACAACTCGAAAGTTACCT
  TCCTTGATTAAATATGGTCACAGTATCAATGAAAATTATATAAAAGATTTGAGCTATGCA
  CTACAATTAATGCGCGATGAAATGCCCACTTCTGATTTATATGATGGCTCGGGCATCATA
  GAAACACCCCACGGTTATATTGTAATGATAGATACCATTTATACATCTAAACAATATGAT
  CAATTATCATCTTTAGATCCTATTGTCGATAGTGCTTATCCATTAGAGCCTAAAGGTACA
  TTTAAAGATTGGTTAAATATGTATATTAACGAAGTCAAAGGTCATCTTCTTCTAGAGTTA
  GCGGTCGTATTTGGAATTTCTGCCTTAGTGACAAGCTTTCTGAAGTACAAACGTGAAATC
  GAATTTGCAGGAATTATCTTTAGTTTTACAGGCCAGTCATCAACTGGTAAATCAACAGCA
  GCAGCTTTAGCAGTATCCGTCGCTGGAAATCCAACTAAAGGTAATCAAACACTTTTTCGA
  AGTTGGAATGCCACACGTAATGCACTTGAAAGTTACTTGAGTAACAATTTTGGTATACCT
  ATTGTATTTGATGAACTCTCATCAGCAACTTTTAAAGATACAACGGGGTTATTGTACTCT
  ATCGCTGAAGGTCAAGGGCGTCAACGTTCCAATGTACACGGAGAAGTTAAAACGCCTAAG
  AATTGGGGCACTTCTGTGATTTCAACCTCTGAATACAGTATCTTTACTGATTCTGCTCAA
  AACGATGGATTACGTGTAAGAACCATCGAGATTAATGAACAATTTACTACAAATGCGACC
  AATGCTGACAATATCAAAAAAGCTGTGGCACTAAACCATGGTCATGTTTTACCGTTAGTA
  GCTCAATACCTTATAAATCGTGAAGATGAAGTTATTCAATGGTTTTACAAGGAAGTAGAT
  TGGTTTGAAGCTAAATTGAACAGTGAAACAAACAATACAGGTATTCGTATGTTTAAGCGT
  TATGCAGTCATTACAACATCTGCGAAAATATTAGGTCGTGTTTTAGCTACAGATATTGAT
  ATCGCTAAAATCAGAGACTACTTTATTGATTATCATGCTCATACAGTCTCTGAACGATCA
  CTGGCAGATAAAGCGATCGAAGTCATTACTCAATTTGTAGCACAAAACCGTGGTAAATTT
  TCTGATGATACAGCGTTAAAGAATATGTTCGAGAACTATGGACTAATTGCCTTAAAAGAT
  AATCATATCGAAGTCAAAATCATTGCGTCTGTATTTAAAAATATGTTGTTAGAGCATCAC
  TTCCAAGATGTTAATAATGTTGTTAATGCGCTAAGGGATAAAGGATTTATCGAATCTGAC
  CATGATCGAATTACAACAAAAAGAACAGTCAAAGATGGCAATGGCAAAAAACAGTCACTT
  GTCTTTTACCATTTAAAGCTAGATTCAGAATATGCGTCTATTTTTGGTCTAACTAAAGAT
  GCTGAACCTATTAAAGCTGAAATTAACTTGGATAACAAAAAGATACTTGAATCATGGAAA
  AAACAGAATGATGATTTAATTGATTTTTAA
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⊟Protein

⊟General

• locus tag: SACOL0043 [new locus tag: SACOL_RS00230 ]
• symbol: SACOL0043
• description: hypothetical protein
• length: 589
• theoretical pI: 6.56253
• theoretical MW: 66710.1
• GRAVY: -0.331919

⊟Function

• TIGRFAM:
• TheSEED  :
  • FIG01108399: hypothetical protein
• PFAM:
  P-loop_NTPase (CL0023) DUF927; Domain of unknown function (DUF927) (PF06048; HMM-score: 212.3)
  and 1 more
  Transthyretin (CL0287) GramPos_pilinD3; Gram-positive pilin backbone subunit 3, Cna-B-like domain (PF16570; HMM-score: 14.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009049
  • TAT(Tat/SPI): 0.000329
  • LIPO(Sec/SPII): 0.003712
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 57652412 NCBI
• RefSeq: YP_184953 NCBI
• UniProt: A0A0H2WZP5 UniProt

⊟Protein sequence

• MSHHDIFRLGLYHLDTMGWHKTIPPKKDGDEKKVVTMSSCMAVEAKFWHVLENTEKIIISNGKDKEHTFDSDILTTRKLPSLIKYGHSINENYIKDLSYALQLMRDEMPTSDLYDGSGIIETPHGYIVMIDTIYTSKQYDQLSSLDPIVDSAYPLEPKGTFKDWLNMYINEVKGHLLLELAVVFGISALVTSFLKYKREIEFAGIIFSFTGQSSTGKSTAAALAVSVAGNPTKGNQTLFRSWNATRNALESYLSNNFGIPIVFDELSSATFKDTTGLLYSIAEGQGRQRSNVHGEVKTPKNWGTSVISTSEYSIFTDSAQNDGLRVRTIEINEQFTTNATNADNIKKAVALNHGHVLPLVAQYLINREDEVIQWFYKEVDWFEAKLNSETNNTGIRMFKRYAVITTSAKILGRVLATDIDIAKIRDYFIDYHAHTVSERSLADKAIEVITQFVAQNRGKFSDDTALKNMFENYGLIALKDNHIEVKIIASVFKNMLLEHHFQDVNNVVNALRDKGFIESDHDRITTKRTVKDGNGKKQSLVFYHLKLDSEYASIFGLTKDAEPIKAEINLDNKKILESWKKQNDDLIDF

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SACOL0043 < SACOL0044

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications