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⊟Summary[edit | edit source]
- pan ID?: SAUPAN003164000
- symbol?: spxA
- synonym:
- description?: transcriptional regulator Spx
- transcriptional regulator Spx
- regulatory protein Spx
- Arsenate reductase family protein
- proabable transcriptional regulator
- Spx family transcriptional regulator
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 3557958..3558389
- synteny block?: BlockID0022520
- occurrence?: in 100% of 33 strains
spxA: stress-induced transcriptional regulator Spx [1]
A crystal structure is available: 5KEF
Under normal conditions, Spx is targeted for proteolysis by YjbH-augmented ClpXP. Under conditions of redox or other stresses, YjbH self-aggregates, Spx is no longer proteolyzed and it binds the alpha subunit where it enhances binding to extended -44 promoter elements.
Consensus Spx -44 extended promoter element: -46 - TCGTAA - -41
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0952
08BA02176:
C248_1020 (spx)
11819-97:
MS7_0953 (spxA)
6850:
RSAU_000873 (spxA)
71193:
ST398NM01_0993
ECT-R 2:
ECTR2_852
ED133:
SAOV_0943
ED98:
SAAV_0958 (spxA)
HO 5096 0412:
SAEMRSA15_08270
JH1:
SaurJH1_1015 (spxA)
JH9:
SaurJH9_0996 (spxA)
JKD6008:
SAA6008_00950 (spxA)
JKD6159:
SAA6159_00854 (spxA)
LGA251:
SARLGA251_09130 (spxA)
M013:
M013TW_0920
MRSA252:
SAR0965 (spxA)
MSHR1132:
SAMSHR1132_08450
MSSA476:
SAS0867 (spxA)
Mu3:
SAHV_0992 (spxA)
Mu50:
SAV0997 (spxA)
MW2:
MW0879 (spxA)
RF122:
SAB0864 (spxA)
ST398:
SAPIG0993
T0131:
SAT0131_01032 (rho)
TCH60:
HMPREF0772_12239 (spxA)
TW20:
SATW20_09950 (spxA)
USA300_TCH1516:
USA300HOU_0955 (spxA)
VC40:
SAVC_04155 (spxA)
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQILKMTEDGTDEIISTR
N315 MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQILKMTEDGTDEIISTR
NCTC8325 MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQILKMTEDGTDEIISTR
Newman MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQILKMTEDGTDEIISTR
USA300_FPR3757 MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQILKMTEDGTDEIISTR
************************************************************
COL SKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQVGYNEDEIRRFLPRKVRT
N315 SKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQVGYNEDEIRRFLPRKVRT
NCTC8325 SKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQVGYNEDEIRRFLPRKVRT
Newman SKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQVGYNEDEIRRFLPRKVRT
USA300_FPR3757 SKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQVGYNEDEIRRFLPRKVRT
************************************************************
COL FQLQEAQRMVD
N315 FQLQEAQRMVD
NCTC8325 FQLQEAQRMVD
Newman FQLQEAQRMVD
USA300_FPR3757 FQLQEAQRMVD
***********
- ↑ Tobias Krogh Nielsen, Ida Birkjær Petersen, Lijuan Xu, Maria Disen Barbuti, Viktor Mebus, Anni Justh, Abdulelah Ahmed Alqarzaee, Nicolas Jacques, Cécile Oury, Vinai Thomas, Morten Kjos, Camilla Henriksen, Dorte Frees
The Spx stress regulator confers high-level β-lactam resistance and decreases susceptibility to last-line antibiotics in methicillin-resistant Staphylococcus aureus.
Antimicrob Agents Chemother: 2024, 68(6);e0033524
[PubMed:38690894] [WorldCat.org] [DOI] (I p)Jing Shi, Fangfang Li, Aijia Wen, Libing Yu, Lu Wang, Fulin Wang, Yuanling Jin, Sha Jin, Yu Feng, Wei Lin
Structural basis of transcription activation by the global regulator Spx.
Nucleic Acids Res: 2021, 49(18);10756-10769
[PubMed:34530448] [WorldCat.org] [DOI] (I p)Jakob Engman, Annika Rogstam, Dorte Frees, Hanne Ingmer, Claes von Wachenfeldt
The YjbH adaptor protein enhances proteolysis of the transcriptional regulator Spx in Staphylococcus aureus.
J Bacteriol: 2012, 194(5);1186-94
[PubMed:22194450] [WorldCat.org] [DOI] (I p)Sünje Johanna Pamp, Dorte Frees, Susanne Engelmann, Michael Hecker, Hanne Ingmer
Spx is a global effector impacting stress tolerance and biofilm formation in Staphylococcus aureus.
J Bacteriol: 2006, 188(13);4861-70
[PubMed:16788195] [WorldCat.org] [DOI] (P p)