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⊟Summary[edit | edit source]
- pan ID?: SAUPAN006083000
- symbol?: —
- synonym:
- description?: helicase
- helicase
- DEAD/DEAH box helicase
- DUF3427 domain-containing protein
- helicase, putative
- putative helicase
- DEAD/H helicase / Type III restriction enzyme, res subunit
- DNA/RNA helicase of DEAD/DEAH box family
- DNA or RNA helicase
- type III restriction enzyme, res subunit
- type III restriction protein res subunit
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 6135884..6138001
- synteny block?: BlockID0048220
- occurrence?: in 85% of 34 strains
sauUSI : 5-(hydroxy)methylcytosine-specific restriction endonuclease SauUSI [1]
• A crystal structure is available: 7CLG
The type IV restriction endonuclease SauUSI binds to to DNA containing a 5'-S5mCNGS-3' target recognition domain. Its cleavage mechanism is complex involving lateral translocation along the DNA accompanied by ATP hydrolysis (analogous to type III restriction endonucleases) but preferential DNA cleavage only when stationary and "hit" by an actively translocating enzyme. This results in cleavage near target recognition domains at low enzyme concentrations due to stationary enzymes bound at TRDs and shredding of DNA at high enzyme concentrations as translocating enzymes bump into each other, stop their translocation, and engage their DNA cleavage as actively-translocating enzymes behind them "pile up".
⊟Orthologs[edit | edit source]
04-02981:
SA2981_2425
08BA02176:
—
11819-97:
MS7_2495
6850:
RSAU_002332
71193:
—
ECT-R 2:
ECTR2_2345
ED133:
SAOV_2534c
ED98:
SAAV_2555
HO 5096 0412:
SAEMRSA15_23880
JH1:
SaurJH1_2567
JH9:
SaurJH9_2515
JKD6008:
SAA6008_02524
JKD6159:
SAA6159_02383
LGA251:
SARLGA251_22700
M013:
M013TW_2454
MRSA252:
SAR2574
MSHR1132:
SAMSHR1132_23100
MSSA476:
SAS2376
Mu3:
SAHV_2472
Mu50:
SAV2488
MW2:
MW2409
RF122:
—
ST398:
—
T0131:
SAT0131_02684 (polC)
TCH60:
HMPREF0772_10702
TW20:
SATW20_26100
USA300_TCH1516:
USA300HOU_2480
VC40:
—
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 | |
| JSNZ |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.505)
COL MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAF
N315 ------------------------------------------------------------
NCTC8325 MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAF
Newman MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAF
USA300_FPR3757 MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAF
JSNZ MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNGKVLSTIIDELQKCETFYFSVAF
COL ITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHA
N315 ------------------------------------------------------------
NCTC8325 ITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHA
Newman ITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHA
USA300_FPR3757 ITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHA
JSNZ ITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHA
COL KGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKNGDLVDSVKSEFDLLWQKSTP
N315 ------------------------------------------------------------
NCTC8325 KGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKNGDLVDSVKSEFDLLWQKSTP
Newman KGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKNGDLVDSVKSEFDLLWQKSTP
USA300_FPR3757 KGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKNGDLVDSVKSEFDLLWQKSTP
JSNZ KGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKNGDLVDSVKSEFDLLWQKSTP
COL LTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDK
N315 ------------------------------------------------------------
NCTC8325 LTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDK
Newman LTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDK
USA300_FPR3757 LTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDK
JSNZ LTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDK
COL AKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDF
N315 ------------------------------------------------------------
NCTC8325 AKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDF
Newman AKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDF
USA300_FPR3757 AKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDF
JSNZ AKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDF
COL GLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFK
N315 ------------------------------------------------------------
NCTC8325 GLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFK
Newman GLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFK
USA300_FPR3757 GLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFK
JSNZ GLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFK
COL PKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESDILCPFHYFGVTDYVHQGIKE
N315 ------------------------------------------------------------
NCTC8325 PKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESDILCPFHYFGVTDYVHQGIKE
Newman PKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESDILCPFHYFGVTDYVHQGIKE
USA300_FPR3757 PKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESDILCPFHYFGVTDYVHQGIKE
JSNZ PKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESDILCPFHYFGVTDYVHQGIKE
COL DDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVA
N315 -------------------------------------MSSKKEAYDLADKLSSKGIKSVA
NCTC8325 DDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVA
Newman DDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVA
USA300_FPR3757 DDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVA
JSNZ DDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVA
:**********************
COL LTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLG
N315 LTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLG
NCTC8325 LTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLG
Newman LTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLG
USA300_FPR3757 LTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLG
JSNZ LTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLG
************************************************************
COL RGLRKSSNKEYVTVIDFIGNYKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINF
N315 RGLRKSANKEYVTVIDFIGNYKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINF
NCTC8325 RGLRKSSNKEYVTVIDFIGNYKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINF
Newman RGLRKSSNKEYVTVIDFIGNYKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINF
USA300_FPR3757 RGLRKSSNKEYVTVIDFIGNYKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINF
JSNZ RGLRKSSNKEYVTVIDFIGNYNTNYLIPIALSGDQSQNKDNYKMFLTNNDSINGVSTINF
******:**************:********************* ****************
COL EEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSN
N315 EEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSN
NCTC8325 EEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSN
Newman EEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSN
USA300_FPR3757 EEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSN
JSNZ EEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSN
************************************************************
COL YYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKML
N315 YYEFLLRYKKIDALLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKML
NCTC8325 YYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKML
Newman YYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKML
USA300_FPR3757 YYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKML
JSNZ YYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKML
*****:******:***********************************************
COL NEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNM
N315 NEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIECNERMIRLSDAFTNALSNQTFKI
NCTC8325 NEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNM
Newman NEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNM
USA300_FPR3757 NEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNM
JSNZ NEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNM
************************************* ********************::
COL FLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPI
N315 FLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIRSQEMPI
NCTC8325 FLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPI
Newman FLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPI
USA300_FPR3757 FLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPI
JSNZ FLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPI
*****************************************************:******
COL FITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQK
N315 FITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLKSKEVQKILSHRAKGIKMYIFVQK
NCTC8325 FITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQK
Newman FITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQK
USA300_FPR3757 FITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQK
JSNZ FITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQK
************************************:***********************
COL KDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN
N315 KDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYITN
NCTC8325 KDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN
Newman KDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN
USA300_FPR3757 KDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN
JSNZ KDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN
**************************************************:**
- ↑ Vinayak Sadasivam Tumuluri, Vrunda Rajgor, Shuang-Yong Xu, Om Prakash Chouhan, Kayarat Saikrishnan
Mechanism of DNA cleavage by the endonuclease SauUSI: a major barrier to horizontal gene transfer and antibiotic resistance in Staphylococcus aureus.
Nucleic Acids Res: 2021, 49(4);2161-2178
[PubMed:33533920] [WorldCat.org] [DOI] (I p)Shuang-Yong Xu, Anna R Corvaglia, Siu-Hong Chan, Yu Zheng, Patrick Linder
A type IV modification-dependent restriction enzyme SauUSI from Staphylococcus aureus subsp. aureus USA300.
Nucleic Acids Res: 2011, 39(13);5597-610
[PubMed:21421560] [WorldCat.org] [DOI] (I p)