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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2431 [new locus tag: SAUSA300_RS13465 ]
  • pan locus tag?: SAUPAN006083000
  • symbol: SAUSA300_2431
  • pan gene symbol?: —
  • synonym:
  • product: putative helicase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2431 [new locus tag: SAUSA300_RS13465 ]
  • symbol: SAUSA300_2431
  • product: putative helicase
  • replicon: chromosome
  • strand: -
  • coordinates: 2614823..2617684
  • length: 2862
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGTAGATTACTAAATGATTTCAATCAATCTTTACATAAAGGGTTTATAGACAAACAT
    ATTTCTCATAAAGGGAATTATACACCGAAGCTTTTAGTAAATAATAAAAATGAAAAAGTT
    CTTTCAACAATTATAGATGAACTTCAAAAATGTGAAACGTTTTATTTTTCTGTGGCTTTT
    ATAACAGAAAGCGGTTTAGCCAGCTTAAAGGCTCAACTATTAGATTTAAGCAATAAGGGT
    GTTAAAGGGAAAATATTAACATCTAATTACTTAGGGTTTAATAGTCCTAAAATGTATGGA
    GAATTACTTAAATTAAAAAATGTAGAGGTAAGATTAACTGATATTGCTGGATTCCATGCC
    AAAGGATATATTTTTGAGCATAAGGATTATAGTTCTATGGTAATAGGAAGCTCTAATTTA
    ACATCTAATGCGTTAAAGGTTAATTATGAGCATAATGTTTTACTGTCTACTATGAAAAAT
    GGCGATTTAGTTGATAGTGTAAAAAGTGAATTTGACTTGTTATGGCAAAAGAGTACCCCA
    CTGACTGAGCAATGGATTAATTCATATAAAGAATCATTTGAGTACCGTTCCTTAGAAAAA
    TTAGCGGAGGTTGAACAAACTCAAATGCTGCTAGCTGATAAGGTGAAAAAATCAGTGGAA
    ATTGTACCCAATTTAATGCAAGCAGAAGCATTAAGGTCATTAAAAGCTATTAGGGATAAA
    GCCAAAGATAAGGCGTTAATAATATCTGCAACTGGTACAGGTAAAACGATTTTATGTGCA
    TTAGATGTTAGAGAAGTAAACCCTAATAAATTTTTATTTATTGTTCATAATGAGGGGATT
    TTAAATAGAGCTAAGGAAGAATTTAAAAAAGTATTACCTATAAAAAATGATAGTGATTTT
    GGATTGTTGACAGGAAAACATAGAGATGTCGATGCCAAATATTTATTTGCAACCATTCAA
    ACATTATCTAGAGATGATAATTTTAAGCAATTTGATGAAAATGAATTTGATTATATTGTT
    TTTGATGAAGCACATCGTTCTGCTGCATCTACTTATCAACGAGTATTTAACTACTTCAAA
    CCTAAGTTCATGCTGGGAATGACTGCTACACCAGAAAGATCAGATGAATTAAGTATATTT
    GAACTGTTTGATTATAATATTGCATATGAAATAAGGTTACAAGCAGCATTAGAAAGTGAT
    ATTTTATGTCCATTTCATTATTTTGGTGTGACTGATTATGTACATCAAGGTATTAAAGAA
    GATGATGTAACTAAACTGAGATATTTAACTTCTGATGAAAGAGTTAATTATATTATTCAA
    AAGACAGATTACTATGGATATTCAGGTGAAATTTTACAAGGATTGATTTTTGTAAGTAGC
    AAAAAAGAAGCTTATGATTTAGCGGATAAACTAAGTTCAAAAGGGATTAAAAGTGTTGCT
    TTAACAGGAGATGATAGTGTTAATTATCGCCAAATTGTAATAGAAAAATTAAAAGAAGGT
    AAGATTAACTATATTATAACTGTAGATCTTTTTAACGAAGGTATTGATATTCCAGAAGTC
    AATCAAGTTGTAATGTTGAGACCAACAGAGTCTAGTATTATATTCATTCAGCAACTTGGT
    AGAGGATTAAGAAAAAGTTCAAATAAAGAGTATGTTACAGTAATTGATTTTATTGGTAAT
    TATAAGACAAATTATTTAATTCCGATTGCGCTTTCTGGGGATCAATCGCAAAATAAAGAT
    AATTATAAGAAGTTTTTAACGAATAACGATTCGATTAATGGAGTATCTACAATTAATTTT
    GAAGAAGTTGCTAAAAAACAGATTTACAATTCATTGGATGCAGTATCATTAAATCAAAAT
    AAATTAATATTAAAAGCTTATGAAGAAGTTGAAAATAGATTGGGACACATGCCGTTACTA
    ATGGATTTCATACAACAACATTCTATAGATCCAAGCGTTATATTTTCTAAATTTAGTAAT
    TATTACGAGTTCTTAGTGAGATATAAAAAAATAGATACATTATTGACGGAAAATGAATCT
    AAAAATCTGGTTTTCTTTTCAAGGCAAATAGCACCTGGATTGAAAAGAATCGATAGTTTA
    GTTTTAGAAGAACTTTTAAAAAATGAATTAACATATGATGAATTAAAAAATAAAATGTTG
    AACGAAGTTAAGGATATAACAGAAGATGATATAGATACTTCATTAAGAATTTTAGATTTT
    TCATTTTACAACGCAGGAATTGAAAAAATCTATGGAAGTCCCATTATAGAACGTAATGAA
    AGAATGATAAGACTAAGCGATGCATTTACTAATGCTCTAAGTAATCAAACATTTAATATG
    TTTTTAGAAGATCTTATTGAATTATCAAAGTATAACAATGAAAAATACCAAAAAGGTAAA
    AATGGACTGATTTTATATAATAAATATTCAAGAGAAGATTTTAGTAAAATATTTAATTGG
    AATAAAAATGGTTCGTCTGTAATCATGGGATACATGATAAAAAGCCAAGAAATGCCAATA
    TTTATCACTTATGATAAGCATGAGGATATAAGTGATAGCACAAAATATGAAGATGAATTT
    TTAAGTCAAGATGAATTAAAATGGTTTACTAAATCTAATAGAACGCTAGAATCAAAAGAA
    GTTCAGAAAATTTTGTCCCATAGAGCAAAAGGAATTAAAATGTATATTTTTGTACAGAAA
    AAAGATGATGATGGTATATATTTTTATTATTTAGGAACTGCTGGATATATAGAAGGTTCA
    GAAAAGCAAGATAAAATGCCGAATGGTTCAAACGTAGTAACGATGGATTTAGCACTGGAT
    AAAGCTGTAAGAGATGATATATATCGTTATCTAACAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2862

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2431 [new locus tag: SAUSA300_RS13465 ]
  • symbol: SAUSA300_2431
  • description: putative helicase
  • length: 953
  • theoretical pI: 6.08051
  • theoretical MW: 109900
  • GRAVY: -0.407765

⊟Function[edit | edit source]

  • TIGRFAM:
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 70.1)
    and 4 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 53.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 24.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 22.1)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 14)
  • TheSEED  :
    • DNA/RNA helicase of DEAD/DEAH box family
  • PFAM:
    no clan defined DUF3427; Domain of unknown function (DUF3427) (PF11907; HMM-score: 268.6)
    and 5 more
    P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 120.8)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 61.7)
    PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 56.4)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 26.5)
    DUF2075; Uncharacterized conserved protein (DUF2075) (PF09848; HMM-score: 20.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00472
    • TAT(Tat/SPI): 0.000194
    • LIPO(Sec/SPII): 0.000364
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSRLLNDFNQSLHKGFIDKHISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKNGDLVDSVKSEFDLLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGEILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITVDLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSSNKEYVTVIDFIGNYKTNYLIPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLILKAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKNLVFFSRQIAPGLKRIDSLVLEELLKNELTYDELKNKMLNEVKDITEDDIDTSLRILDFSFYNAGIEKIYGSPIIERNERMIRLSDAFTNALSNQTFNMFLEDLIELSKYNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIKSQEMPIFITYDKHEDISDSTKYEDEFLSQDELKWFTKSNRTLESKEVQKILSHRAKGIKMYIFVQKKDDDGIYFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYLTN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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