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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0147 [new locus tag: SAUSA300_RS00770 ]
  • pan locus tag?: SAUPAN000959000
  • symbol: SAUSA300_0147
  • pan gene symbol?: —
  • synonym:
  • product: 5' nucleotidase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0147 [new locus tag: SAUSA300_RS00770 ]
  • symbol: SAUSA300_0147
  • product: 5' nucleotidase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 166604..168139
  • length: 1536
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGTCAAACATAGCATTTTATGTCGTGAGTGACGTACATGGTTATATTTTCCCAACAGAT
    TTTACGAGTAGAAATCAATATCAACCTATGGGATTGTTACTAGCGAATCATGTTATAGAA
    CAAGACAGAAGGCAGTATGACCAAAGTTTTAAAATAGATAATGGTGATTTTTTGCAAGGG
    TCACCATTTTGTAATTACTTAATCGCGCATAGCGGCAGTAGCCAGCCTTTAGTTGATTTT
    TATAATCGAATGGCATTCGACTTTGGTACGCTTGGTAATCATGAATTTAATTATGGATTA
    CCATACTTAAAAGACACTTTACGCAGACTCAATTATCCAGTTTTGTGCGCTAATATATAT
    GAAAATGATAGTACATTGACTGATAACGGTGTGAAGTATTTTCAGGTTGGAGATCAAACT
    GTTGGTGTGATAGGTTTAACGACACAATTTATTCCCCATTGGGAACAACCAGAGCATATT
    CAGTCACTTACGTTTCATAGTGCTTTTGAAATACTTCAACAATACTTACCTGAAATGAAG
    CGACATGCAGATATCATTGTGGTTTGTTACCATGGTGGATTTGAAAAGGATTTAGAAAGT
    GGTACGCCGACCGAAGTATTAACGGGTGAAAATGAAGGATATGCCATGTTAGAAGCGTTT
    TCTAAAGATATAGATATCTTTATTACGGGTCACCAACATCGACAAATTGCTGAAAGGTTT
    AAGCAAACGGCTGTGATTCAACCTGGTACGAGAGGTACAACTGTAGGCAGAGTAGTCTTG
    AGTACTGATGAATATGAAAATCTATCCGTTGAATCATGTGAATTACTTCCTGTTATAGAT
    GATTCCACATTTACTATTGATGAAGATGACCAACATTTACGAAAGCAGTTAGAGGACTGG
    TTAGATTACGAAATTACTACATTGCCATATGATATGACGATTAATCATGCATTTGAGGCA
    CGTGTGGCACCGCATCCTTTTACAAATTTTATGAATTACGCTTTATTAGAAAAAAGTGAC
    GCAGATGTTGCCTGTACAGCTTTGTTTGATTCTGCTAGTGGTTTCAAGCAAGTCGTGACG
    ATGCGAGATGTTATTAACAATTACCCATTTCCAAATACATTTAAAGTTTTAGCTGTAAGT
    GGTGCCAAACTTAAAGAAGCCATTGAACGATCAGCAGAATATTTTGACGTGAAAAATGAT
    GAAGTTAGTGTGAGCGCAGACTTCCTTGAACCCAAACCACAACACTTTAATTATGATATA
    TATGGTGGCGTAAGTTATACCATTCATGTTGGAAGACCAAAGGGACAACGTGTGAGCAAT
    ATGATGATTCAAGGTCACGCAGTTGATTTAAAGCAGACATATACAATTTGTGTAAATAAT
    TATCGTGCAGTAGGCGGTGGTCAGTATGATATGTATATCGACGCGCCAGTTGTAAAAGAT
    ATTCAAGTTGAAGGCGCACAATTACTTATTGATTTTTTATCAAATAATAATTTGATGCGC
    ATCCCGCAAGTTGTTGATTTTAAAGTTGAAAAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1536

⊟Protein[edit | edit source]

Protein Data Bank: 3QFK
Protein Data Bank: 4Q7F

⊟General[edit | edit source]

  • locus tag: SAUSA300_0147 [new locus tag: SAUSA300_RS00770 ]
  • symbol: SAUSA300_0147
  • description: 5' nucleotidase family protein
  • length: 511
  • theoretical pI: 4.62258
  • theoretical MW: 58109.9
  • GRAVY: -0.297847

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.4.16?  ExPASy
    2',3'-cyclic-nucleotide 2'-phosphodiesterase Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 3'-phosphate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other 2',3'-cyclic-nucleotide 2'-phosphodiesterase (TIGR01390; EC 3.1.4.16; HMM-score: 170.6)
    and 4 more
    thiosulfohydrolase SoxB (TIGR04486; HMM-score: 83.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 56.5)
    Metabolism Transport and binding proteins Other NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 56.5)
    Unknown function Enzymes of unknown specificity phosphodiesterase, MJ0936 family (TIGR00040; EC 3.1.-.-; HMM-score: 17.2)
  • TheSEED  :
    • 2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)
    Nucleosides and Nucleotides Purines Purine conversions  2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)
    and 2 more
    Nucleosides and Nucleotides Pyrimidines pyrimidine conversions  2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)
    Potassium metabolism Potassium metabolism - no subcategory Glutathione-regulated potassium-efflux system and associated functions  2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)
  • PFAM:
    no clan defined 5_nucleotid_C; 5'-nucleotidase, C-terminal domain (PF02872; HMM-score: 98.1)
    and 2 more
    Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 30.1)
    Metallophos_2; Calcineurin-like phosphoesterase superfamily domain (PF12850; HMM-score: 14.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.11
    • Cytoplasmic Membrane Score: 0.02
    • Cellwall Score: 8.97
    • Extracellular Score: 0.9
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005944
    • TAT(Tat/SPI): 0.000207
    • LIPO(Sec/SPII): 0.001346
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRRQYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPYLKDTLRRLNYPVLCANIYENDSTLTDNGVKYFQVGDQTVGVIGLTTQFIPHWEQPEHIQSLTFHSAFEILQQYLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLEAFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGRVVLSTDEYENLSVESCELLPVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYALLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSAEYFDVKNDEVSVSADFLEPKPQHFNYDIYGGVSYTIHVGRPKGQRVSNMMIQGHAVDLKQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFKVEK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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