PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0275 [new locus tag: SAUSA300_RS01475 ]
• pan locus tag^?: SAUPAN001171000
• symbol: SAUSA300_0275
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0275 [new locus tag: SAUSA300_RS01475 ]
• symbol: SAUSA300_0275
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 327963..328616
• length: 654
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912775 NCBI
• RefSeq: YP_492989 NCBI
• BioCyc: see SAUSA300_RS01475
• MicrobesOnline: 1291790 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGAATTGAAAGTCGATGATTTTGTAAAGAATATAAAAAGACCATACTTGACTGTATTG
  GGAGTATTTGTAGTTGCAGTTTCATTATTTTTTGACGCGATTATGTTTTTCTACGCTAAA
  CTATATGATAAATTGCCTATGTATTTACTGGTGTTTATGGCCTTTACAGCTGTAATTTTG
  ATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTT
  AGATATCTCACACTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATT
  TACGTTTTTGGTGTAGTCGGTTTTGGATTTGATGTTTTCAATTATTGTCTAGGTATTATC
  TTGATGTTATTTATTTCTTGGTTAGGTTTATTTTTATTTTATAAAAACGAATTTGATAGT
  GAAAATCCTAATCCTGCAGTTAATGCCATAGCAATTATTATAAAATTATTTGCTTTTGGA
  GGAATATTTTATATAAGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGG
  TTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTAC
  GCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAA
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  654
  

⊟Protein

⊟General

• locus tag: SAUSA300_0275 [new locus tag: SAUSA300_RS01475 ]
• symbol: SAUSA300_0275
• description: hypothetical protein
• length: 217
• theoretical pI: 6.71765
• theoretical MW: 25183
• GRAVY: 0.82765

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Photosynthesis cytochrome b559, alpha subunit (TIGR01332; HMM-score: 11.9)
  and 1 more
  exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase (TIGR03025; HMM-score: 3.2)
• TheSEED  :
  • FIG01108255: hypothetical protein
• PFAM:
  no clan defined DUF5079; Domain of unknown function (DUF5079) (PF16882; HMM-score: 243.2)
  and 8 more
  DUF2157; Predicted membrane protein (DUF2157) (PF09925; HMM-score: 17.6)
  GPCR_A (CL0192) Ceramidase; Ceramidase (PF05875; HMM-score: 15.5)
  no clan defined DUF3624; Protein of unknown function (DUF3624) (PF12292; HMM-score: 12.8)
  DUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 11.4)
  P12; Virus attachment protein p12 family (PF12669; HMM-score: 10)
  DUF3180; Protein of unknown function (DUF3180) (PF11377; HMM-score: 8.7)
  DUF3397; Protein of unknown function (DUF3397) (PF11877; HMM-score: 8.2)
  Jiraiya; Jiraiya (PF15038; HMM-score: 5.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000557
  • TAT(Tat/SPI): 0.000116
  • LIPO(Sec/SPII): 0.008764
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 87161065 NCBI
• RefSeq: YP_492989 NCBI
• UniProt: A0A0H2XFX1 UniProt

⊟Protein sequence

• MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDKLPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYVFGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFGGIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0275 > SAUSA300_0276

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications