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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00254
  • pan locus tag?: SAUPAN001171000
  • symbol: SAOUHSC_00254
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00254
  • symbol: SAOUHSC_00254
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 273373..274026
  • length: 654
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGAATTGAAAGTCGATGATTTTGTAAAGAATATAAAAAGACCATACTTGACTGTATTG
    GGAGTATTTGTAGTTGCAGTTTCATTATTTTTTGACGCGATTATGTTTTTCTACGCTAAA
    CTATATGATAAATTGCCTATGTATTTACTGGTGTTTATGGCCTTTACAGCTGTAATTTTG
    ATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTT
    AGATATCTCACACTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATT
    TACGTTTTTGGTGTAGTCGGTTTTGGATTTGATGTTTTCAATTATTGTCTAGGTATTATC
    TTGATGTTATTTATTTCTTGGTTAGGTTTATTTTTATTTTATAAAAACGAATTTGATAGT
    GAAAATCCTAATCCTGCAGTTAATGCCATAGCAATTATTATAAAATTATTTGCTTTTGGA
    GGAATATTTTATATAAGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGG
    TTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTAC
    GCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    654

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00254
  • symbol: SAOUHSC_00254
  • description: hypothetical protein
  • length: 217
  • theoretical pI: 6.71765
  • theoretical MW: 25183
  • GRAVY: 0.82765

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Photosynthesis cytochrome b559, alpha subunit (TIGR01332; HMM-score: 11.9)
    and 1 more
    exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase (TIGR03025; HMM-score: 3.2)
  • TheSEED  :
    • FIG01108255: hypothetical protein
  • PFAM:
    no clan defined DUF5079; Domain of unknown function (DUF5079) (PF16882; HMM-score: 243.2)
    and 8 more
    DUF2157; Predicted membrane protein (DUF2157) (PF09925; HMM-score: 17.6)
    GPCR_A (CL0192) Ceramidase; Ceramidase (PF05875; HMM-score: 15.5)
    no clan defined DUF3624; Protein of unknown function (DUF3624) (PF12292; HMM-score: 12.8)
    DUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 11.4)
    P12; Virus attachment protein p12 family (PF12669; HMM-score: 10)
    DUF3180; Protein of unknown function (DUF3180) (PF11377; HMM-score: 8.7)
    DUF3397; Protein of unknown function (DUF3397) (PF11877; HMM-score: 8.2)
    Jiraiya; Jiraiya (PF15038; HMM-score: 5.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000557
    • TAT(Tat/SPI): 0.000116
    • LIPO(Sec/SPII): 0.008764
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDKLPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYVFGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFGGIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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