PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0286 [new locus tag: SAUSA300_RS01530 ]
• pan locus tag^?: SAUPAN001187000
• symbol: SAUSA300_0286
• pan gene symbol^?: esaE
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0286 [new locus tag: SAUSA300_RS01530 ]
• symbol: SAUSA300_0286
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 341159..341833
• length: 675
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915066 NCBI
• RefSeq: YP_493000 NCBI
• BioCyc: see SAUSA300_RS01530
• MicrobesOnline: 1291801 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAGATGTTAAGCGAATAGATTATTTTTCTTACGAAGAATTAACAATTTTAGGTGGT
  AGTAAATTGCCTCTCGTAAATTTTGAATTGTTTGATCCATCAAATTTTGAAGAAGCTAAA
  GCTGCTTTAATTGAAAAGGAATTAGTAACAGAGAATGACAAGTTAACTGATGCAGGTTTT
  AAAGTGGCTACATTAGTCAGAGAGTATATTAGCGCCATTGTAAATATTCGAATTAATGAT
  ATGTATTTTGCACCATTTAGCTATGAAAAAGATGAATATATTTTGTTAAGCCGGTTTAAA
  AATAATGGGTTTCAAATACGAATTATCAATAAAGACATTGCATGGTGGTCGATTGTACAA
  TCATATCCTTTATTGATGAGACAAGAAAAGTCCAATGATTGGGACTTTAAACAAATTGAC
  GATGAAACATTGGAGAACTTAAATAATGAAAGTATCGATACGATTGGGCGTGTTTTAGAA
  ATTGAAATATACAATCATCAAGGTGACCCTCAACAAAGTTTATATAACATTTATGAACAA
  AATGATTTGTTATTCATTCGATACCCATTAAAAGATAAAGTGCTGAATGTTCATATTGGT
  GTCATTAATACATTTATACGAGAATTATTTGGATTCGATACTGATGAAAATCATATTAAT
  AAAGCAGAGGAGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0286 [new locus tag: SAUSA300_RS01530 ]
• symbol: SAUSA300_0286
• description: hypothetical protein
• length: 224
• theoretical pI: 4.33817
• theoretical MW: 26411.6
• GRAVY: -0.394643

⊟Function

• TIGRFAM:
• TheSEED  :
  • SAV0291 homolog within ESAT-6 gene cluster
  Membrane Transport Protein secretion system, Type VII ESAT-6 proteins secretion system in Firmicutes  SAV0291 homolog within ESAT-6 gene cluster
• PFAM:
  no clan defined DUF5081; Domain of unknown function (DUF5081) (PF16887; HMM-score: 285.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.014497
  • TAT(Tat/SPI): 0.000187
  • LIPO(Sec/SPII): 0.000337
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160631 NCBI
• RefSeq: YP_493000 NCBI
• UniProt: A0A0H2XFI6 UniProt

⊟Protein sequence

• MKDVKRIDYFSYEELTILGGSKLPLVNFELFDPSNFEEAKAALIEKELVTENDKLTDAGFKVATLVREYISAIVNIRINDMYFAPFSYEKDEYILLSRFKNNGFQIRIINKDIAWWSIVQSYPLLMRQEKSNDWDFKQIDDETLENLNNESIDTIGRVLEIEIYNHQGDPQQSLYNIYEQNDLLFIRYPLKDKVLNVHIGVINTFIRELFGFDTDENHINKAEE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0279 > SAUSA300_0280 > SAUSA300_0281 > SAUSA300_0282 > SAUSA300_0283 > SAUSA300_0284 > SAUSA300_0285 > SAUSA300_0286 > SAUSA300_0287 > SAUSA300_0288 > SAUSA300_0289

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Mark Anderson, Yi-Hsing Chen, Emily K Butler, Dominique M Missiakas
EsaD, a secretion factor for the Ess pathway in Staphylococcus aureus.
J Bacteriol: 2011, 193(7);1583-9
[PubMed:21278286] [WorldCat.org] [DOI] (I p)Zhenping Cao, M Guillermina Casabona, Holger Kneuper, James D Chalmers, Tracy Palmer
The type VII secretion system of Staphylococcus aureus secretes a nuclease toxin that targets competitor bacteria.
Nat Microbiol: 2016, 2;16183
[PubMed:27723728] [WorldCat.org] [DOI] (I e)