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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0288 [new locus tag: SAUSA300_RS01540 ]
  • pan locus tag?: SAUPAN001190000
  • symbol: SAUSA300_0288
  • pan gene symbol?: esaD
  • synonym:
  • product: hypothetical protein

suggest symbol of "essD", synonym of "esaD" and product of "Type VII-secreted nuclease virulence factor" to align with PMID 27795323 and 35960642

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0288 [new locus tag: SAUSA300_RS01540 ]
  • symbol: SAUSA300_0288
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 342160..344004
  • length: 1845
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGACAAAAGATATTGAATATCTAACAGCTGATTATGACAATGAAAAGTCATCTATCCAA
    AGTGTAATAGATGCAATAGAGGGTCAAGACTTCTTAGATGTAGATACAACAATGGATGAT
    GCGGTAAGCGATGTCAGTTCTTTAGACGAAGATGGCGCAATATCATTAACAAGTAGTGTA
    GTAGGTCCACAAGGATCTAAATTAATGGGGTATTATCAAAATGAGTTATATGATTATGCA
    TCTCAATTAGATTCGAAAATGAAAGAAATTATTGACACGCCATTTATAGAAGATATAGAT
    AAAGCATTCAAAGGTATAACGAATGTTAAATTGGAAAATATACTAATTAAAAATGGTGGT
    GGACATGGTAGAGATACCTATGGGGCTTCTGGGAAAATTGCAAAGGGAGATGCCAAGAAA
    AGTGACAGCGATGTTTATAGCATCGATGAAATATTAAAATCGGATCAAGAATTTGTAAAA
    GTAATTGATCAGCATTACAAAGAAATGAAAAAAGAAGATAAGAAATTATCTAAAAGTGAT
    TTTGAAAAAATGATGACTCAGGGCGCTTCTTGTGATTACATGACAGTAGCTGAAGCGGAA
    GAGCTAGAGGAGCAAAAGAAAAAAGAAGAAGCTATAGAGATTGCAGCACTAGCTGGTATG
    GTAGTTTTATCTTGTATTAATCCTGTTGCTGGAGCAGTAGCTATTGGTGCTTATTCCGCT
    TATTCAGCAGCAAATGCAGCCACAGGAAAAAATATTGTAACTGGAAGAAAGCTATCTAAA
    GAAGAACGAATCATGGAAGGACTTTCGCTTATTCCATTGCCAGGTATGGGCTTCCTCAAA
    GGTGCTGGGAAAAGTTTAATGAAATTAGGCTTCAAAGGCGGAGAAAAATTTGCAGTTAAA
    ACAGGATTGCAAAAGACAATGCAACAAGCAGTTAGTCGTATTTCACCTAAAATGGGAATG
    ATGAAAAACAGTGTGTTGAATCAATCTCGTAACTTTGCTCAAAATACTCATGTTGGACAA
    ATGCTGAGTAACATGCGTGGTCAAGCAACTCATACTGTTCAACAAAGTAGAAATTGGATT
    GGACAACAAGCACAAAATGTCAAACGAATAGTGAATAATGGACTTGATAAAGAAATAGCA
    CATCCATTTAAACAACAACTTGCACCAGCGGGAATGGGTGGTATAAAATTTGCTGAAACA
    ACTACTTTGAGAAACATGGGTCAAAACATAAAGCGTGCTGTTACACCACAAAATCACGTG
    ACACATGGTCCAAAAGATAGTATGGTGAGAAGTGAAGGCAAACATAGTATAAGTAGCCAT
    GAAATGAATTCATCAAAGTATGTTGAATCACCAAACTACACCAAGGTTGAATTCGGAGAA
    CACTATGCAAGACTTAGACCTAAGAAACTAAAGGCGAATATTGAATACACAACACCTACT
    GGTCACATATATCGAACCGATCATAAAGGTCGCATAAAAGAAGTTTATGTAGACAATCTC
    TCTCTAAAAGATGGGGATCGTAATAGCCATGCACAAAGAACTGTGGGGGGAGAGGATAGA
    TTACCAGACGATGATGGAGGTCACTTAATCGCTAGAATGTTTGGTGGTTCAAAAGACATT
    GATAACCTTGTGGCACAAAGTAAATTTATCAACCGTCCATTTAAGGAGAAAGGTCATTGG
    TATAATCTTGAGAAAGAGTGGCAAGAGTTCTTAAACTCTGGGAAAGAGGTGAAAAATATT
    AAAATGGAAGTAAAATATAGCGGTAATAGTCAAAGACCGACTATATTTAAAGTTGAATAT
    GAAATTAATGGTGAAAGAAATATTAGAAGAATATTAAATAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1845

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0288 [new locus tag: SAUSA300_RS01540 ]
  • symbol: SAUSA300_0288
  • description: hypothetical protein
  • length: 614
  • theoretical pI: 8.8881
  • theoretical MW: 68317.9
  • GRAVY: -0.65684

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Lmo0066 homolog within ESAT-6 gene cluster, similarity to ADP-ribosylating toxins
    Membrane Transport Protein secretion system, Type VII ESAT-6 proteins secretion system in Firmicutes  Lmo0066 homolog within ESAT-6 gene cluster, similarity to ADP-ribosylating toxins
  • PFAM:
    His-Me_finger (CL0263) Endonuclea_NS_2; DNA/RNA non-specific endonuclease (PF13930; HMM-score: 108.9)
    and 3 more
    no clan defined PT-TG; Pre-toxin TG (PF14449; HMM-score: 34.1)
    FAM180; FAM180 family (PF15173; HMM-score: 13.1)
    EpsM (CL0331) Phage_lysis; Bacteriophage Rz lysis protein (PF03245; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00495
    • TAT(Tat/SPI): 0.000477
    • LIPO(Sec/SPII): 0.000561
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSDVSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKAFKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVKVIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEELEEQKKKEEAIEIAALAGMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGMGFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQNTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGMGGIKFAETTTLRNMGQNIKRAVTPQNHVTHGPKDSMVRSEGKHSISSHEMNSSKYVESPNYTKVEFGEHYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGDRNSHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKFINRPFKEKGHWYNLEKEWQEFLNSGKEVKNIKMEVKYSGNSQRPTIFKVEYEINGERNIRRILNK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Mark Anderson, Yi-Hsing Chen, Emily K Butler, Dominique M Missiakas
EsaD, a secretion factor for the Ess pathway in Staphylococcus aureus.
J Bacteriol: 2011, 193(7);1583-9
[PubMed:21278286] [WorldCat.org] [DOI] (I p)Zhenping Cao, M Guillermina Casabona, Holger Kneuper, James D Chalmers, Tracy Palmer
The type VII secretion system of Staphylococcus aureus secretes a nuclease toxin that targets competitor bacteria.
Nat Microbiol: 2016, 2;16183
[PubMed:27723728] [WorldCat.org] [DOI] (I e)

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