PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0289 [new locus tag: SAUSA300_RS01545 ]
• pan locus tag^?: SAUPAN001199000
• symbol: SAUSA300_0289
• pan gene symbol^?: esaG1
• synonym: esaG
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0289 [new locus tag: SAUSA300_RS01545 ]
• symbol: SAUSA300_0289
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 344015..344506
• length: 492
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915305 NCBI
• RefSeq: YP_493003 NCBI
• BioCyc: see SAUSA300_RS01545
• MicrobesOnline: 1291804 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGACATTTGAAGAGAAGCTTAGCAAAATATACAATGAAATTGCGAATGAGATTAGCAGT
  ATGATACCGGTAGAGTGGGAAAAAGTATATACAATGGCTTATATAGATGATGGAGGAGGT
  GAAGTATTCTTTAATTATACTAAACCAGGTAGTGATGACTTGAATTATTACACCAATATA
  CCTAAGGAGTATAACATTTCTGTGCAAGTATTTGATGATTTATGGATGGATTTATATGAT
  TTATTTGAGGAGTTAAGAGATTTATTTAAAGAAGAAGATTTAGAACCATGGACATCATGC
  GAATTTGATTTTACAAGAGAAGGTGAATTAAAAGTTTCATTTGATTATATTGATTGGATA
  AATTCAGAATTTGGTCAAATAGGTCGACAAAATTACTATAAGTATAGAAAATTTGGAATT
  TTACCAGAAACGGAATATGAAATTAATAAAGTTAAAGAAATCGAGCAATATATTAAAGAG
  CTAGAAGAATAA
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  492
  

⊟Protein

⊟General

• locus tag: SAUSA300_0289 [new locus tag: SAUSA300_RS01545 ]
• symbol: SAUSA300_0289
• description: hypothetical protein
• length: 163
• theoretical pI: 3.90747
• theoretical MW: 19731.8
• GRAVY: -0.668098

⊟Function

• TIGRFAM:
  conserved hypothetical protein (TIGR01741; HMM-score: 257.5)
• TheSEED  :
  • Repetitive hypothetical protein near ESAT cluster, SA0282 homolog
  Membrane Transport Protein secretion system, Type VII ESAT-6 proteins secretion system in Firmicutes  Repetitive hypothetical protein near ESAT cluster, SA0282 homolog
• PFAM:
  no clan defined DUF600; Protein of unknown function, DUF600 (PF04634; HMM-score: 169.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009447
  • TAT(Tat/SPI): 0.000426
  • LIPO(Sec/SPII): 0.000752
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161334 NCBI
• RefSeq: YP_493003 NCBI
• UniProt: A0A0H2XH25 UniProt

⊟Protein sequence

• MTFEEKLSKIYNEIANEISSMIPVEWEKVYTMAYIDDGGGEVFFNYTKPGSDDLNYYTNIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEDLEPWTSCEFDFTREGELKVSFDYIDWINSEFGQIGRQNYYKYRKFGILPETEYEINKVKEIEQYIKELEE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0279 > SAUSA300_0280 > SAUSA300_0281 > SAUSA300_0282 > SAUSA300_0283 > SAUSA300_0284 > SAUSA300_0285 > SAUSA300_0286 > SAUSA300_0287 > SAUSA300_0288 > SAUSA300_0289

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Zhenping Cao, M Guillermina Casabona, Holger Kneuper, James D Chalmers, Tracy Palmer
The type VII secretion system of Staphylococcus aureus secretes a nuclease toxin that targets competitor bacteria.
Nat Microbiol: 2016, 2;16183
[PubMed:27723728] [WorldCat.org] [DOI] (I e)