PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0282 [new locus tag: SA_RS01645 ]
• pan locus tag^?: SAUPAN001199000
• symbol: SA0282
• pan gene symbol^?: esaG1
• synonym: esaG
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0282 [new locus tag: SA_RS01645 ]
• symbol: SA0282
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 340566..341057
• length: 492
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123061 NCBI
• RefSeq: NP_373528 NCBI
• BioCyc: see SA_RS01645
• MicrobesOnline: 102554 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACATTTGAAGAGAAGCTTAGCAAAATATACAATGAAATTGCGAATGAGATTAGCAAT
  ATGATACCGGTAGAGTGGGAAAAAGTATATACAATGGCTTATATAGATGATGGAGGAGGG
  GAGGTATTCTTTAATTATACTAAGCCAGGTAGTGATGACTTGAATTATTACACCGATATA
  CCTAAGGAGTATAACATTTCTGTGCAAGTATTTGATGATTTATGGATGGATTTATATGAT
  TTGTTTGAGGAGTTAAGAGATTTATTTAAAGAAGAAGATTTAGAACCATGGACATCATGT
  GAATTTGACTTTACAAGAGAGGGTAAATTGAAAGTATCATTTGATTATATAGATTGGATA
  AATACAGAGTTTGATCAATTGGGCCGTGAAAATTATTATATGTACAAAAAATTTGGGGTT
  ATACCAGAAATGGAATATGAAATGGAGGAGGTTAAACAAATCGAGCAATATATTAAAGAG
  CAAGAAGAATAG
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  492
  

⊟Protein

⊟General

• locus tag: SA0282 [new locus tag: SA_RS01645 ]
• symbol: SA0282
• description: hypothetical protein
• length: 163
• theoretical pI: 3.80294
• theoretical MW: 19871
• GRAVY: -0.710429

⊟Function

• TIGRFAM:
  conserved hypothetical protein (TIGR01741; HMM-score: 262.2)
• TheSEED  :
  • Repetitive hypothetical protein near ESAT cluster, SA0282 homolog
  Membrane Transport Protein secretion system, Type VII ESAT-6 proteins secretion system in Firmicutes  Repetitive hypothetical protein near ESAT cluster, SA0282 homolog
• PFAM:
  no clan defined DUF600; Protein of unknown function, DUF600 (PF04634; HMM-score: 175.9)
  and 3 more
  DUF1031; Protein of unknown function (DUF1031) (PF06275; HMM-score: 13.6)
  Chalcone-like (CL0560) Chalcone; Chalcone-flavanone isomerase (PF02431; HMM-score: 11.5)
  no clan defined RTX; RTX N-terminal domain (PF02382; HMM-score: 10)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008518
  • TAT(Tat/SPI): 0.000318
  • LIPO(Sec/SPII): 0.000607
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15925995 NCBI
• RefSeq: NP_373528 NCBI
• UniProt: A0A0H3JKL1 UniProt

⊟Protein sequence

• MTFEEKLSKIYNEIANEISNMIPVEWEKVYTMAYIDDGGGEVFFNYTKPGSDDLNYYTDIPKEYNISVQVFDDLWMDLYDLFEELRDLFKEEDLEPWTSCEFDFTREGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVIPEMEYEMEEVKQIEQYIKEQEE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0272 > SA0273 > SA0274 > SA0275 > SA0276 > SA0277 > SA0278 > SA0279 > SA0280 > SA0281 > SA0282

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Zhenping Cao, M Guillermina Casabona, Holger Kneuper, James D Chalmers, Tracy Palmer
The type VII secretion system of Staphylococcus aureus secretes a nuclease toxin that targets competitor bacteria.
Nat Microbiol: 2016, 2;16183
[PubMed:27723728] [WorldCat.org] [DOI] (I e)