PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0280 [new locus tag: SA_RS01635 ]
• pan locus tag^?: SAUPAN001188000
• symbol: SA0280
• pan gene symbol^?: esxD
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0280 [new locus tag: SA_RS01635 ]
• symbol: SA0280
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 338384..338701
• length: 318
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123059 NCBI
• RefSeq: NP_373526 NCBI
• BioCyc: see SA_RS01635
• MicrobesOnline: 102552 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  
  ATGACGTTGAGTGGAAAAATTAGTGTTAAAGCTGAAACGATTGCACATGTTGTAAAAGAA
  TTGGAAAGCATTAGTCAAAAGTATGATGAAATAGCTCAAAACTTTGGAAAAATAGCGCAA
  TTAAATTACTACAGTAGTGAAAAAGCTGCACATTCTATGGAAAATGGCTATAGTAGTGCT
  GCAACAGTCATTAGTGGTCTCAAAGGTCCATTGAGTACACTCGGTGGTGGCGTCATGAAT
  TCAGCACAAAAGTTCTTTGAAGCAGATGAACATTGGGGTACGGAATTTGCCAAGCTTTAC
  TATAATATTGAGGGATAG
  60
  120
  180
  240
  300
  318
  

⊟Protein

⊟General

• locus tag: SA0280 [new locus tag: SA_RS01635 ]
• symbol: SA0280
• description: hypothetical protein
• length: 105
• theoretical pI: 5.61271
• theoretical MW: 11414.7
• GRAVY: -0.28381

⊟Function

• TIGRFAM:
  WXG100 family type VII secretion target (TIGR03930; HMM-score: 13)
• TheSEED  :
  • FIG01108475: hypothetical protein
• PFAM:
  no clan defined Dynactin_p22; Dynactin subunit p22 (PF07426; HMM-score: 13)
  DUF5098; Domain of unknown function (DUF5098) (PF17023; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011527
  • TAT(Tat/SPI): 0.001116
  • LIPO(Sec/SPII): 0.001213
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15925993 NCBI
• RefSeq: NP_373526 NCBI
• UniProt: A0A0H3JLA5 UniProt

⊟Protein sequence

• MTLSGKISVKAETIAHVVKELESISQKYDEIAQNFGKIAQLNYYSSEKAAHSMENGYSSAATVISGLKGPLSTLGGGVMNSAQKFFEADEHWGTEFAKLYYNIEG

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0272 > SA0273 > SA0274 > SA0275 > SA0276 > SA0277 > SA0278 > SA0279 > SA0280 > SA0281 > SA0282

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Mark Anderson, Khaled A Aly, Yi-Hsing Chen, Dominique Missiakas
Secretion of atypical protein substrates by the ESAT-6 secretion system of Staphylococcus aureus.
Mol Microbiol: 2013, 90(4);734-43
[PubMed:24033479] [WorldCat.org] [DOI] (I p)