From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0645 [new locus tag: SAUSA300_RS03460 ]
  • pan locus tag?: SAUPAN002538000
  • symbol: SAUSA300_0645
  • pan gene symbol?: graR
  • synonym:
  • product: DNA-binding response regulator

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0645 [new locus tag: SAUSA300_RS03460 ]
  • symbol: SAUSA300_0645
  • product: DNA-binding response regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 719018..719692
  • length: 675
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCAAATACTACTAGTAGAAGATGACAATACTTTGTTTCAAGAATTGAAAAAAGAATTA
    GAACAATGGGATTTTAATGTTGCTGGTATTGAAGATTTCGGCAAAGTAATGGATACATTT
    GAAAGTTTTAATCCTGAAATTGTTATATTGGATGTTCAATTACCTAAATATGATGGGTTT
    TATTGGTGCAGAAAAATGAGAGAAGTTTCCAACGTACCAATATTATTTTTATCATCTCGT
    GATAATCCAATGGATCAAGTGATGAGTATGGAACTTGGCGCAGATGATTATATGCAAAAA
    CCGTTCTATACCAATGTATTAATTGCTAAATTACAAGCGATTTATCGTCGTGTCTATGAG
    TTTACAGCTGAAGAAAAACGTACATTGACTTGGCAAGATGCTGTCGTTGATCTATCAAAA
    GATAGTATACAAAAAGGTGATCAGACGATTTTCCTGTCCAAAACAGAAATGATTATATTA
    GAAATTCTTATTACCAAAAAAAATCAAATCGTTTCGAGAGATACAATTATCACTGCATTA
    TGGGATGATGAAGCATTTGTTAGTGATAATACGTTAACAGTAAATGTGAATCGTTTACGA
    AAAAAATTATCTGAAATTAGTATGGATAGTGCAATCGAAACAAAAGTAGGAAAAGGATAT
    ATGGCTCATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    675

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0645 [new locus tag: SAUSA300_RS03460 ]
  • symbol: SAUSA300_0645
  • description: DNA-binding response regulator
  • length: 224
  • theoretical pI: 4.39684
  • theoretical MW: 26078.8
  • GRAVY: -0.257589

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 146.5)
    Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 146.5)
    Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 118.3)
    and 7 more
    proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 116.9)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 45)
    Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 45)
    Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 45)
    Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 44.2)
    Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 38)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 19.4)
  • TheSEED:  
    Virulence, Disease and Defense Bacteriocins, ribosomally synthesized antibacterial peptides Bacitracin Stress Response  Two-component response regulator BceR
  • PFAM:
    HTH (CL0123) Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 76.8)
    CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 76.7)
    and 1 more
    POTRA (CL0191) POTRA_1; POTRA domain, FtsQ-type (PF08478; HMM-score: 13.5)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001883
    • TAT(Tat/SPI): 0.000104
    • LIPO(Sec/SPII): 0.00034
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIILEILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]