PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0645 [new locus tag: SAUSA300_RS03460 ]
• pan locus tag^?: SAUPAN002538000
• symbol: SAUSA300_0645
• pan gene symbol^?: graR
• synonym:
• product: DNA-binding response regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0645 [new locus tag: SAUSA300_RS03460 ]
• symbol: SAUSA300_0645
• product: DNA-binding response regulator
• replicon: chromosome
• strand: +
• coordinates: 719018..719692
• length: 675
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914136 NCBI
• RefSeq: YP_493348 NCBI
• BioCyc: see SAUSA300_RS03460
• MicrobesOnline: 1292160 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGCAAATACTACTAGTAGAAGATGACAATACTTTGTTTCAAGAATTGAAAAAAGAATTA
  GAACAATGGGATTTTAATGTTGCTGGTATTGAAGATTTCGGCAAAGTAATGGATACATTT
  GAAAGTTTTAATCCTGAAATTGTTATATTGGATGTTCAATTACCTAAATATGATGGGTTT
  TATTGGTGCAGAAAAATGAGAGAAGTTTCCAACGTACCAATATTATTTTTATCATCTCGT
  GATAATCCAATGGATCAAGTGATGAGTATGGAACTTGGCGCAGATGATTATATGCAAAAA
  CCGTTCTATACCAATGTATTAATTGCTAAATTACAAGCGATTTATCGTCGTGTCTATGAG
  TTTACAGCTGAAGAAAAACGTACATTGACTTGGCAAGATGCTGTCGTTGATCTATCAAAA
  GATAGTATACAAAAAGGTGATCAGACGATTTTCCTGTCCAAAACAGAAATGATTATATTA
  GAAATTCTTATTACCAAAAAAAATCAAATCGTTTCGAGAGATACAATTATCACTGCATTA
  TGGGATGATGAAGCATTTGTTAGTGATAATACGTTAACAGTAAATGTGAATCGTTTACGA
  AAAAAATTATCTGAAATTAGTATGGATAGTGCAATCGAAACAAAAGTAGGAAAAGGATAT
  ATGGCTCATGAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0645 [new locus tag: SAUSA300_RS03460 ]
• symbol: SAUSA300_0645
• description: DNA-binding response regulator
• length: 224
• theoretical pI: 4.39684
• theoretical MW: 26078.8
• GRAVY: -0.257589

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 146.5)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 146.5)
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 118.3)
  and 7 more
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 116.9)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 45)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 45)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 45)
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 44.2)
  Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 38)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 19.4)
• TheSEED  :
  • Two-component response regulator BceR
  Virulence, Disease and Defense Bacteriocins, ribosomally synthesized antibacterial peptides Bacitracin Stress Response  Two-component response regulator BceR
• PFAM:
  HTH (CL0123) Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 76.8)
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 76.7)
  and 1 more
  POTRA (CL0191) POTRA_1; POTRA domain, FtsQ-type (PF08478; HMM-score: 13.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by GraR*, response regulator important in cationic antimicrobial peptide (CAMP) resistance: in NCTC8325

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001883
  • TAT(Tat/SPI): 0.000104
  • LIPO(Sec/SPII): 0.00034
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160864 NCBI
• RefSeq: YP_493348 NCBI
• UniProt: Q2FIY0 UniProt

⊟Protein sequence

• MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIILEILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0644 > SAUSA300_0645 > SAUSA300_0646

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications