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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00665
  • pan locus tag?: SAUPAN002538000
  • symbol: SAOUHSC_00665
  • pan gene symbol?: graR
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00665
  • symbol: SAOUHSC_00665
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 652088..652762
  • length: 675
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCAAATACTACTAGTAGAAGATGACAATACTTTGTTTCAAGAATTGAAAAAAGAATTA
    GAACAATGGGATTTTAATGTTGCTGGTATTGAAGATTTCGGCAAAGTAATGGATACATTT
    GAAAGTTTTAATCCTGAAATTGTTATATTGGATGTTCAATTACCTAAATATGATGGGTTT
    TATTGGTGCAGAAAAATGAGAGAAGTTTCCAACGTACCAATATTATTTTTATCATCTCGT
    GATAATCCAATGGATCAAGTGATGAGTATGGAACTTGGCGCAGATGATTATATGCAAAAA
    CCGTTCTATACCAATGTATTAATTGCTAAATTACAAGCGATTTATCGTCGTGTCTATGAG
    TTTACAGCTGAAGAAAAACGTACATTGACTTGGCAAGATGCTGTCGTTGATCTATCAAAA
    GATAGTATACAAAAAGGTGATCAGACGATTTTCCTGTCCAAAACAGAAATGATTATATTA
    GAAATTCTTATTACCAAAAAAAATCAAATCGTTTCGAGAGATACAATTATCACTGCATTA
    TGGGATGATGAAGCATTTGTTAGTGATAATACGTTAACAGTAAATGTGAATCGTTTACGA
    AAAAAATTATCTGAAATTAGTATGGATAGTGCAATCGAAACAAAAGTAGGAAAAGGATAT
    ATGGCTCATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    675

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00665
  • symbol: SAOUHSC_00665
  • description: hypothetical protein
  • length: 224
  • theoretical pI: 4.39684
  • theoretical MW: 26078.8
  • GRAVY: -0.257589

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 146.5)
    Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 146.5)
    Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 118.3)
    and 7 more
    proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 116.9)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 45)
    Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 45)
    Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 45)
    Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 44.2)
    Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 38)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 19.4)
  • TheSEED  :
    • Two-component response regulator BceR
    Virulence, Disease and Defense Bacteriocins, ribosomally synthesized antibacterial peptides Bacitracin Stress Response  Two-component response regulator BceR
  • PFAM:
    HTH (CL0123) Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 76.8)
    CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 76.7)
    and 1 more
    POTRA (CL0191) POTRA_1; POTRA domain, FtsQ-type (PF08478; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001883
    • TAT(Tat/SPI): 0.000104
    • LIPO(Sec/SPII): 0.00034
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIILEILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [4] [5]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Min Li, Yuping Lai, Amer E Villaruz, David J Cha, Daniel E Sturdevant, Michael Otto
    Gram-positive three-component antimicrobial peptide-sensing system.
    Proc Natl Acad Sci U S A: 2007, 104(22);9469-74
    [PubMed:17517597] [WorldCat.org] [DOI] (P p)
  2. ↑ Silvia Herbert, Agnieszka Bera, Christiane Nerz, Dirk Kraus, Andreas Peschel, Christiane Goerke, Michael Meehl, Ambrose Cheung, Friedrich Götz
    Molecular basis of resistance to muramidase and cationic antimicrobial peptide activity of lysozyme in staphylococci.
    PLoS Pathog: 2007, 3(7);e102
    [PubMed:17676995] [WorldCat.org] [DOI] (I p)
  3. ↑ Mélanie Falord, Ulrike Mäder, Aurélia Hiron, Michel Débarbouillé, Tarek Msadek
    Investigation of the Staphylococcus aureus GraSR regulon reveals novel links to virulence, stress response and cell wall signal transduction pathways.
    PLoS One: 2011, 6(7);e21323
    [PubMed:21765893] [WorldCat.org] [DOI] (I p)
  4. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  5. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  6. ↑ 6.0 6.1 6.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Michael Meehl, Silvia Herbert, Friedrich Götz, Ambrose Cheung
Interaction of the GraRS two-component system with the VraFG ABC transporter to support vancomycin-intermediate resistance in Staphylococcus aureus.
Antimicrob Agents Chemother: 2007, 51(8);2679-89
[PubMed:17502406] [WorldCat.org] [DOI] (P p)Silvia Herbert, Agnieszka Bera, Christiane Nerz, Dirk Kraus, Andreas Peschel, Christiane Goerke, Michael Meehl, Ambrose Cheung, Friedrich Götz
Molecular basis of resistance to muramidase and cationic antimicrobial peptide activity of lysozyme in staphylococci.
PLoS Pathog: 2007, 3(7);e102
[PubMed:17676995] [WorldCat.org] [DOI] (I p)Dirk Kraus, Silvia Herbert, Sascha A Kristian, Arya Khosravi, Victor Nizet, Friedrich Götz, Andreas Peschel
The GraRS regulatory system controls Staphylococcus aureus susceptibility to antimicrobial host defenses.
BMC Microbiol: 2008, 8;85
[PubMed:18518949] [WorldCat.org] [DOI] (I e)

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