PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0717 [new locus tag: SAUSA300_RS03850 ]
• pan locus tag^?: SAUPAN002631000
• symbol: nrdF
• pan gene symbol^?: nrdF
• synonym:
• product: ribonucleotide-diphosphate reductase subunit beta

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0717 [new locus tag: SAUSA300_RS03850 ]
• symbol: nrdF
• product: ribonucleotide-diphosphate reductase subunit beta
• replicon: chromosome
• strand: +
• coordinates: 796357..797328
• length: 972
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914251 NCBI
• RefSeq: YP_493420 NCBI
• BioCyc: see SAUSA300_RS03850
• MicrobesOnline: 1292232 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGATAGCTGTTAATTGGAACACACAAGAAGATATGACGAATATGTTTTGGAGACAAAAT
  ATATCTCAAATGTGGGTTGAAACAGAATTTAAAGTATCAAAAGACATTGCAAGTTGGAAG
  ACTTTATCTGAAGCTGAACAAGACACATTTAAAAAAGCATTAGCTGGTTTAACAGGCTTA
  GATACACATCAAGCAGATGATGGCATGCCTTTAGTTATGCTACATACGACTGACTTAAGG
  AAAAAAGCAGTTTATTCATTTATGGCGATGATGGAGCAAATACACGCGAAAAGCTATTCA
  CATATTTTCACAACACTATTACCATCTAGTGAAACAAACTACCTATTAGATGAATGGGTT
  TTAGAGGAACCCCATTTAAAATATAAATCTGATAAAATTGTTGCTAATTATCACAAACTT
  TGGGGTAAAGAAGCTTCGATATACGACCAATATATGGCCAGAGTTACGAGTGTATTTTTA
  GAAACATTCTTATTCTTCTCAGGTTTCTATTATCCACTATATCTTGCTGGTCAAGGGAAA
  ATGACGACATCAGGTGAAATCATTCGTAAAATTCTTTTAGATGAATCTATTCATGGTGTA
  TTTACCGGTTTAGATGCACAGCATTTACGAAATGAACTATCTGAAAGTGAGAAACAAAAA
  GCAGATCAAGAAATGTATAAATTGCTAAATGACTTGTATTTAAATGAAGAGTCATACACA
  AAAATGTTATACGATGATCTTGGAATCACTGAAGATGTGCTAAACTATGTTAAATATAAT
  GGAAACAAAGCACTTTCAAACTTAGGCTTTGAACCTTATTTTGAGGAACGTGAATTTAAC
  CCAATCATTGAGAATGCCTTAGATACAACAACTAAAAACCATGACTTCTTCTCAGTAAAA
  GGTGATGGTTATGTATTAGCATTAAACGTAGAAGCATTACAAGATGATGACTTTGTATTT
  GACAACAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0717 [new locus tag: SAUSA300_RS03850 ]
• symbol: NrdF
• description: ribonucleotide-diphosphate reductase subunit beta
• length: 323
• theoretical pI: 4.52439
• theoretical MW: 37513.1
• GRAVY: -0.409907

⊟Function

• reaction:
  EC 1.17.4.1?  ExPASy
  Ribonucleoside-diphosphate reductase 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H₂O = ribonucleoside diphosphate + thioredoxin
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism ribonucleoside-diphosphate reductase, class 1b, beta subunit (TIGR04171; EC 1.17.4.1; HMM-score: 411.6)
• TheSEED  :
  • Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1)
  Nucleosides and Nucleotides Nucleosides and Nucleotides - no subcategory Ribonucleotide reduction  Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1)
• PFAM:
  Ferritin (CL0044) Ribonuc_red_sm; Ribonucleotide reductase, small chain (PF00268; HMM-score: 220.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helix: 1
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003485
  • TAT(Tat/SPI): 0.000504
  • LIPO(Sec/SPII): 0.000367
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 87162072 NCBI
• RefSeq: YP_493420 NCBI
• UniProt: A0A0H2XI26 UniProt

⊟Protein sequence

• MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSEAEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHIFTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFLETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEKQKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEEREFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: nrdI > SAUSA300_0716 > nrdF

⊟Regulation

• regulator: NrdR (repression) regulon

  NrdR

  (TF)

  important in Deoxyribonucleotide biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications