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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0687 [new locus tag: SA_RS03915 ]
  • pan locus tag?: SAUPAN002631000
  • symbol: nrdF
  • pan gene symbol?: nrdF
  • synonym:
  • product: ribonucleotide-diphosphate reductase subunit beta

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0687 [new locus tag: SA_RS03915 ]
  • symbol: nrdF
  • product: ribonucleotide-diphosphate reductase subunit beta
  • replicon: chromosome
  • strand: +
  • coordinates: 785694..786665
  • length: 972
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATAGCTGTTAATTGGAACACACAAGAAGATATGACGAATATGTTTTGGAGACAAAAT
    ATATCTCAAATGTGGGTTGAAACAGAATTTAAAGTATCAAAAGACATTGCAAGTTGGAAG
    ACTTTATCTGAAGCTGAACAAGACACATTTAAAAAAGCATTAGCTGGTTTAACAGGCTTA
    GATACACATCAAGCAGATGATGGCATGCCTTTAGTTATGCTACATACGACTGACTTAAGG
    AAAAAAGCAGTTTATTCATTTATGGCGATGATGGAGCAAATACACGCGAAAAGCTATTCA
    CATATTTTCACAACACTATTACCATCTAGTGAAACAAACTACCTATTAGATGAATGGGTT
    TTAGAGGAACCCCATTTAAAATATAAATCTGATAAAATTGTTGCTAATTATCACAAACTT
    TGGGGTAAAGAAGCTTCGATATACGACCAATATATGGCCAGAGTTACGAGTGTATTTTTA
    GAAACATTCTTATTCTTCTCAGGTTTCTATTATCCACTATATCTTGCTGGTCAAGGGAAA
    ATGACGACATCAGGTGAAATCATTCGTAAAATTCTTTTAGATGAATCTATTCATGGTGTA
    TTTACCGGTTTAGATGCACAGCATTTACGAAATGAACTATCTGAAAGTGAGAAACAAAAA
    GCAGATCAAGAAATGTATAAATTGCTAAATGACTTGTATTTAAATGAAGAGTCATACACA
    AAAATGTTATACGATGATCTTGGAATCACTGAAGATGTGCTAAACTATGTTAAATATAAT
    GGAAACAAAGCACTTTCAAACTTAGGCTTTGAACCTTATTTTGAGGAACGTGAATTTAAC
    CCAATCATTGAGAATGCCTTAGATACAACAACTAAAAACCATGACTTCTTCTCAGTAAAA
    GGTGATGGTTATGTATTAGCATTAAACGTAGAAGCATTACAAGATGATGACTTTGTATTT
    GACAACAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    972

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0687 [new locus tag: SA_RS03915 ]
  • symbol: NrdF
  • description: ribonucleotide-diphosphate reductase subunit beta
  • length: 323
  • theoretical pI: 4.52439
  • theoretical MW: 37513.1
  • GRAVY: -0.409907

Function[edit | edit source]

  • reaction:
    EC 1.17.4.1?  ExPASy
    Ribonucleoside-diphosphate reductase 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism ribonucleoside-diphosphate reductase, class 1b, beta subunit (TIGR04171; EC 1.17.4.1; HMM-score: 411.6)
  • TheSEED:  
    Nucleosides and Nucleotides Nucleosides and Nucleotides - no subcategory Ribonucleotide reduction  Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1)
  • PFAM:
    Ferritin (CL0044) Ribonuc_red_sm; Ribonucleotide reductase, small chain (PF00268; HMM-score: 220.2)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helix: 1
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003485
    • TAT(Tat/SPI): 0.000504
    • LIPO(Sec/SPII): 0.000367
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSEAEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHIFTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFLETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEKQKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEEREFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: NrdR (repression) regulon
    NrdR(TF)important in Deoxyribonucleotide biosynthesis; RegPrecise  

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]