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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0800 [new locus tag: SAUSA300_RS04320 ]
  • pan locus tag?: SAUPAN002842000
  • symbol: sek
  • pan gene symbol?: sek
  • synonym:
  • product: enterotoxin K

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0800 [new locus tag: SAUSA300_RS04320 ]
  • symbol: sek
  • product: enterotoxin K
  • replicon: chromosome
  • strand: -
  • coordinates: 883304..884032
  • length: 729
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAAAAATTAATAGTTATCATATTAATAAATATAATAACTTTAAGTGTCTCTAATAGT
    GCCAGCGCTCAAGGTGATATAGGAATTGATAATCTCAGGAATTTTTATACAAAAAAAGAC
    TTTGTAGATTTAAAAGATGTAAAAGACAATGATACTCCTATAGCTAATCAACTACAATTT
    TCAAATGTATCTTATGATTTAATTTCAGAATCAAAAGATTTTAATAAATTTAGTAATTTC
    AAGGGAAAAAAACTTGATGTTTTTGGTATTAGTTATAATGGCCAGTGTAACAATAAATAC
    ATATATGGTGGAGTCACAGCTACTAACGAATATCTAGATAAACCTAGAAATATCCCTATA
    AATATATGGATCAATGGAAATCACAAAACTATTTCTACCAATAAAGTTTCGACAAATAAA
    AAATTTGTTACCGCTCAAGAGATTGATGTCAAATTAAGAAAGTACCTTCAAGAAGAATAC
    AACATTTATGGACATAACGGCACTAAAAAAGGAGAAGAATATGGTCATAAATCAAAATTT
    TATTCTGGGTTTAATATTGGTAAAGTAACGTTCCATTTAAATAATAATGACACTTTTTCA
    TATGATTTATTCTACACAGGAGATGATGGGTTACCAAAAAGTTTTTTAAAAATTTACGAA
    GACAATAAAACTGTAGAGTCTGAGAAATTCCATTTGGATGTCGATATTTCTTATAAAGAA
    ACGATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    729

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0800 [new locus tag: SAUSA300_RS04320 ]
  • symbol: Sek
  • description: enterotoxin K
  • length: 242
  • theoretical pI: 8.7193
  • theoretical MW: 27750
  • GRAVY: -0.592562

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Superantigen enterotoxin SEK
    Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Superantigen enterotoxin SEK
  • PFAM:
    Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 119.7)
    and 1 more
    OB_enterotoxin (CL0658) Stap_Strp_toxin; Staphylococcal/Streptococcal toxin, OB-fold domain (PF01123; HMM-score: 22.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helix: 1
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NSASAQGD
  • SignalP: Signal peptide SP(Sec/SPI) length 23 aa
    • SP(Sec/SPI): 0.995536
    • TAT(Tat/SPI): 0.000575
    • LIPO(Sec/SPII): 0.002981
    • Cleavage Site: CS pos: 23-24. ASA-QG. Pr: 0.8059
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKLIVIILINIITLSVSNSASAQGDIGIDNLRNFYTKKDFVDLKDVKDNDTPIANQLQFSNVSYDLISESKDFNKFSNFKGKKLDVFGISYNGQCNNKYIYGGVTATNEYLDKPRNIPINIWINGNHKTISTNKVSTNKKFVTAQEIDVKLRKYLQEEYNIYGHNGTKKGEEYGHKSKFYSGFNIGKVTFHLNNNDTFSYDLFYTGDDGLPKSFLKIYEDNKTVESEKFHLDVDISYKETI

Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]