PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0801 [new locus tag: SAUSA300_RS04325 ]
• pan locus tag^?: SAUPAN002843000
• symbol: seq
• pan gene symbol^?: seq
• synonym:
• product: enterotoxin Q

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0801 [new locus tag: SAUSA300_RS04325 ]
• symbol: seq
• product: enterotoxin Q
• replicon: chromosome
• strand: -
• coordinates: 884056..884784
• length: 729
• essential: unknown

⊟Accession numbers

• Gene ID: 3912764 NCBI
• RefSeq: YP_493501 NCBI
• BioCyc: see SAUSA300_RS04325
• MicrobesOnline: 1292316 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAATAAAATATTTAGAGTACTCACTGTTAGCTTGTTTTTCTTCACATTTTTAATAAAA
  AACAATCTAGCATATGCTGATGTAGGGGTAATCAACCTTAGAAACTTTTATACTAATTAT
  CAACCAGAAACGCTTCAAGGAGTTAGTTCTGGAAATTTTTCTACTTCTCATCAATTAGAG
  TATATTGATGGAAAATACACTTTATATTCACAGTTTCATAATGAATATGAAGCGAAGAGA
  TTAAAAGATCATAAAGTAGATATCTTTGGAATAAGTTACTCAGGTCTTTGTAATACAAAA
  TATATGTATGGCGGAATTACGTTGGCGAATCAAAATTTAGATAAACCTAGAAATATACCT
  ATTAATCTCTGGGTCAATGGTAAGCAAAACACTATATCTACAGACGAGGTTTCTACTCAA
  AAAAAAGAGGTAACTGCTCAAGAGATTGATATTAAGTTACGAAAATACCTACAAAACGAA
  TACAATATATATGGTTTTAATAAAACAAAAAAAGGTCAGGAATATGGATATCAGTCAAAA
  TTTAATTCTGGATTTAACAAAGGAAAAATTACTTTCCATTTAAATAATGAAACTTCTTTT
  ACATACGATTTGTTTTACACCGGAACTGGTCAAGCAGAGAGTTTTTTAAAAATTTACAAT
  GATAATAAAACTATAGATGCAGAGAATTTTCATTTGGATGTAGAGATTTCATATGAGAAG
  ACTGAATAA
  60
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  729
  

⊟Protein

⊟General

• locus tag: SAUSA300_0801 [new locus tag: SAUSA300_RS04325 ]
• symbol: Seq
• description: enterotoxin Q
• length: 242
• theoretical pI: 8.29638
• theoretical MW: 28146.3
• GRAVY: -0.621488

⊟Function

• TIGRFAM:
• TheSEED  :
  • Superantigen enterotoxin SEL
  Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Superantigen enterotoxin SEL
• PFAM:
  Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 123.1)
  and 3 more
  OB_enterotoxin (CL0658) Stap_Strp_toxin; Staphylococcal/Streptococcal toxin, OB-fold domain (PF01123; HMM-score: 28.9)
  no clan defined POC3_POC4; 20S proteasome chaperone assembly proteins 3 and 4 (PF10448; HMM-score: 13.5)
  Ant-toxin_C (CL0386) MAP; MAP domain (PF03642; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helices: 0
• LocateP: N-terminally anchored (with CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: NLAYADVG
• SignalP: Signal peptide SP(Sec/SPI) length 26 aa
  • SP(Sec/SPI): 0.989953
  • TAT(Tat/SPI): 0.001928
  • LIPO(Sec/SPII): 0.000619
  • Cleavage Site: CS pos: 26-27. AYA-DV. Pr: 0.9854
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 87161054 NCBI
• RefSeq: YP_493501 NCBI
• UniProt: A0A0H2XFV8 UniProt

⊟Protein sequence

• MNKIFRVLTVSLFFFTFLIKNNLAYADVGVINLRNFYTNYQPETLQGVSSGNFSTSHQLEYIDGKYTLYSQFHNEYEAKRLKDHKVDIFGISYSGLCNTKYMYGGITLANQNLDKPRNIPINLWVNGKQNTISTDEVSTQKKEVTAQEIDIKLRKYLQNEYNIYGFNKTKKGQEYGYQSKFNSGFNKGKITFHLNNETSFTYDLFYTGTGQAESFLKIYNDNKTIDAENFHLDVEISYEKTE

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: sek < seq < SAUSA300_0802 < SAUSA300_0803

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications