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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0819 [new locus tag: SAUSA300_RS04415 ]
  • pan locus tag?: SAUPAN002895000
  • symbol: sufD
  • pan gene symbol?: sufD
  • synonym:
  • product: FeS assembly protein SufD

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0819 [new locus tag: SAUSA300_RS04415 ]
  • symbol: sufD
  • product: FeS assembly protein SufD
  • replicon: chromosome
  • strand: +
  • coordinates: 898628..899935
  • length: 1308
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACAACTGATATTTTGAACATTTCTGAAGAACAACTTGTTGATTATTCTAAAGCCCAC
    AATGAACCTTCTTGGATGACAGAATTACGTAAAAAAGCTTTGAAATTAACAGAAACTTTA
    GAAATGCCAAAACCTGATAAAACAAAATTAAGAAAATGGGATTTTGATTCTTTTAAACAA
    CACGATGTAAAAGGTGATGTTTATCAATCTTTATCACAATTACCTGAGTCAGTAAGAGAA
    ATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTAC
    ACACAAGTTGATGATAATGCATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCT
    CTTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAAGATGCAGTAACAGTAGAT
    GAACATCGTATCACAGCGCTACACACGGCATTAGTTAATGGTGGCGTATTTGTTTATGTT
    CCTAAAAATGTAGTTGTAGAACATCCAGTACAATACGTTGTGTTGCACGACGACGAAAAT
    GCAAGCTTTTATAACCATGTTATCATCGTTACTGAAGAAAGCGCCGAAGTCACATATGTT
    GAAAATTACTTATCAAATGCATCTGGTGAAGGAAATCAATTAAATATTATTTCTGAAGTG
    ATTGCTGGTGCAAATTCAAATATCACATATGGCTCAGTGGACTATATGGATAAAGGCTTT
    ACAGGTCATATCATTCGACGTGGTATTACTGAAGCGGATGCCTCAATTAATTGGGCACTA
    GGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGT
    TCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACA
    TCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATG
    AAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAGCATGGTGGAACTAAA
    TCAATTGCTAATCAGGAATCACGTGTATTAATGTTATCTGAACATGCTCGTGGTGACGCG
    AATCCTATTTTATTAATTGATGAAGATGATGTACAAGCTGGTCATGCTGCATCAGTAGGT
    CGTGTTGATCCAGATCAACTTTACTATTTAATGAGTCGTGGTATTTCTCAAAGAGAAGCG
    GAACGTCTTGTTATACATGGTTTCTTAGATCCAGTAGTACGTGAATTACCTATCGAAGAC
    GTTAAACGTCAATTGAGAGAAGTAATTGAACGCAAAGTTTCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1308

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0819 [new locus tag: SAUSA300_RS04415 ]
  • symbol: SufD
  • description: FeS assembly protein SufD
  • length: 435
  • theoretical pI: 5.20929
  • theoretical MW: 48548.3
  • GRAVY: -0.353103

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly protein SufD (TIGR01981; HMM-score: 239.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly protein SufB (TIGR01980; HMM-score: 232.9)
  • TheSEED  :
    • Iron-sulfur cluster assembly protein SufD
  • PFAM:
    no clan defined UPF0051; Uncharacterized protein family (UPF0051) (PF01458; HMM-score: 195.1)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003227
    • TAT(Tat/SPI): 0.000212
    • LIPO(Sec/SPII): 0.000472
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPKPDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQVDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYVPKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIISEVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNLFGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYIKHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMSRGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: PerR* (repression) regulon
    PerR*(TF)important in Oxidative stress response; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]