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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0851 [new locus tag: SAUSA300_RS04595 ]
  • pan locus tag?: SAUPAN003049000
  • symbol: mnhE
  • pan gene symbol?: mnhE1
  • synonym:
  • product: putative monovalent cation/H+ antiporter subunit E

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0851 [new locus tag: SAUSA300_RS04595 ]
  • symbol: mnhE
  • product: putative monovalent cation/H+ antiporter subunit E
  • replicon: chromosome
  • strand: -
  • coordinates: 928957..929436
  • length: 480
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGGCTGTACAATTAGTATTAAACTTTATTATCGCAGTGTTTTGGTTGTTTGTGACAAAT
    AGTTACACAACAAATAACTTTGTGTTAGGTTTCATCTTTGGCCTAGTTCTTGTTTACCTC
    TTACACAGAGTGTTACCAGGTAGATTTTATGTCATCACGCTATATCGTATTATTAAATTA
    GTTATTATTTTCTTAATCGAACTGATTAAAGCTAATTTTGATGTTTTGAAAATTATTATT
    AAACCTTCGATTAAAAATGAACCAGGATTCTTTGTTTATCACACAGATTTAAAAAAAGAT
    TGGCAAATTGTCTTGCTGTCTAATTTAATTACATTAACACCTGGTACTGTAGTGCTTGGT
    GTAAGTGACGATCGTACAAAAATTTATATTCATGCCATAGATTTTTCAACTAAAGAGCAA
    GAAGTAGAAAGTATTAAAACATCATTAGAGAAAATTGTAAGAGAGGTGGGCGAAATATAA
    60
    120
    180
    240
    300
    360
    420
    480

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0851 [new locus tag: SAUSA300_RS04595 ]
  • symbol: MnhE
  • description: putative monovalent cation/H+ antiporter subunit E
  • length: 159
  • theoretical pI: 9.30363
  • theoretical MW: 18318.7
  • GRAVY: 0.693082

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds multicomponent Na+:H+ antiporter (TIGR00942; HMM-score: 231.1)
  • TheSEED  :
    • Na(+) H(+) antiporter subunit E (TC 2.A.63.1.3)
    Membrane Transport Uni- Sym- and Antiporters Sodium Hydrogen Antiporter  Na(+) H(+) antiporter subunit E (TC 2.A.63.1.3)
  • PFAM:
    no clan defined MNHE; Na+/H+ ion antiporter subunit (PF01899; HMM-score: 143.2)
    and 1 more
    DUF659; Protein of unknown function (DUF 659) (PF04937; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003764
    • TAT(Tat/SPI): 0.000247
    • LIPO(Sec/SPII): 0.0758
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRVLPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQIVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Manisha Vaish, Alexa Price-Whelan, Tamara Reyes-Robles, Jun Liu, Amyeo Jereen, Stephanie Christie, Francis Alonzo, Meredith A Benson, Victor J Torres, Terry A Krulwich
    Roles of Staphylococcus aureus Mnh1 and Mnh2 Antiporters in Salt Tolerance, Alkali Tolerance, and Pathogenesis.
    J Bacteriol: 2018, 200(5);
    [PubMed:29263099] [WorldCat.org] [DOI] (I e)
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