⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0957 [new locus tag: SAUSA300_RS05145 ]
- pan locus tag?: SAUPAN003263000
- symbol: SAUSA300_0957
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0957 [new locus tag: SAUSA300_RS05145 ]
- symbol: SAUSA300_0957
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 1048806..1049276
- length: 471
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3915010 NCBI
- RefSeq: YP_493655 NCBI
- BioCyc: see SAUSA300_RS05145
- MicrobesOnline: 1292472 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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361
421ATGAGTAGAAAAACATACGAAAAGATTGCAAATATAAATGGCATGTTTAATATGTTAGAA
CAACAAATCATTCATAGCCAAGATATGGCTCATTTTAGAAGTGAATTTTTTTACGTCAAT
CATGAGCATCGAGAAAACTATGAAGCACTCCTAATTTATTACAAAAATAGTATCGACAAT
CCTATTGTAGATGGTGCATGTTATATTTTAGCCCTACCTGAAATTTTCAATAGTGTTGAT
GTTTTCGAATCAGAGTTACCATTTTCATGGGTATATGATGAAAATGGCATTACCGAAACA
ATGAAATCACTTAGCATTCCATTACAATATTTAGTTGCAGCAGCTTTAGAAGTAACTGAT
GTGAATATATTTAAGCCTTCAGGATTTACAATGGGAATGAATAATTGGAATATTGCTCAA
ATGCGAATCTTTTGGCAATATACAGCAATTATTAGAAAAGAAGCACTATAA60
120
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300
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420
471
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0957 [new locus tag: SAUSA300_RS05145 ]
- symbol: SAUSA300_0957
- description: hypothetical protein
- length: 156
- theoretical pI: 4.62139
- theoretical MW: 18267.8
- GRAVY: -0.098718
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004126
- TAT(Tat/SPI): 0.000174
- LIPO(Sec/SPII): 0.000299
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHRENYEALLIYYKNSIDNPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMKSLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
The article "High-throughput transposon sequencing highlights the cell wall as an important barrier for osmotic stress in methicillin resistant Staphylococcus aureus and underlines a tailored response to different osmotic stressors" identified the DUF2538 domain containing gene SAUSA300_0957 (gene 957) as essential under salt stress. [1]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Christopher F Schuster, David M Wiedemann, Freja C M Kirsebom, Marina Santiago, Suzanne Walker, Angelika Gründling
High-throughput transposon sequencing highlights the cell wall as an important barrier for osmotic stress in methicillin resistant Staphylococcus aureus and underlines a tailored response to different osmotic stressors.
Mol Microbiol: 2020, 113(4);699-717
[PubMed:31770461] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]
Christopher F Schuster, David M Wiedemann, Freja C M Kirsebom, Marina Santiago, Suzanne Walker, Angelika Gründling
High-throughput transposon sequencing highlights the cell wall as an important barrier for osmotic stress in methicillin resistant Staphylococcus aureus and underlines a tailored response to different osmotic stressors.
Mol Microbiol: 2020, 113(4);699-717
[PubMed:31770461] [WorldCat.org] [DOI] (I p)