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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0958 [new locus tag: SAUSA300_RS05150 ]
  • pan locus tag?: SAUPAN003264000
  • symbol: SAUSA300_0958
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

lcpB: peptidoglycan:teichoic acid ligase B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0958 [new locus tag: SAUSA300_RS05150 ]
  • symbol: SAUSA300_0958
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1049324..1050541
  • length: 1218
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATAAATTTTTAAAATACTTTTTGATCCTTCTAGCATTGGTTCTCATTGTTGTTCCA
    ATAGTCTTCGCTACGTTACTTTTTAAAACGTCCCAAGATGCATTCGAATCTTCTCAAGAC
    AGCAAAAATGCTAATCGTCAATCCAACTTAAGAGATAATAAAGTCAATCCCGAAGAACAA
    CCTATCTCTATTCTTTTCTTAGGTATCGATGATAACGATGGAAGAAGAAAAAAAGGACAG
    GATGCTGAACACTCTAGATCAGACGCTATGATTTTAACTACTTTTAATCAATCAAAACAT
    CAAATTAGAATGCTTAGTATTCCACGTGATACTATCAGCTACATTCCTAAAGTTGGCTAT
    TACGATAAGATTACCCATGCACATGCATATGGTGGTCCAATTGCCGCAATGGATTCGGTA
    GAAGCTACCATGAATGTACCTGTTGATTATTATGTGAGAGTCAATATGAAAGCATTTGTC
    GAGGCTGTAAATGAGCTTGGTGGCATATACTATGATGTGCCATACGACTTAAATGAACCT
    AACACAGATGATACTGGTAAGATTAAAATTAAAAAAGGGTACCAAAAATTAAATGGTGAT
    GAGGCTTTAGCAGTTGCTAGAACTAGACATCATGATTCAGACTTAAAACGTGGACAACGT
    CAAATGGAATTAATTAAGATTTTATTCCAAAAAGCACAGGAAGTTGATTCTATTGATAAA
    CTTGATAACGTGATTCAAATTGTAGGTAAAAATGCAAAGCATAATTTAACTAACTCTGAA
    ATTAAAGCTTTAGCAAAAATGTACTTAACAAATGATGTTGAAATTAAAACTGCGCAATTA
    AAAGGTAAAGATGATATGTTAAATGGTATTTACTATTATCATCCAAGTGTTGAAAGCATT
    CAAAAATATGCAAACTTACTTCGTAAAGACTTAGAATTATCACCTATTAATGATAAAAAT
    GATTTCTTAGATCAACGTGTCATTAATCATTATGGTTCATTAATACCATTAACGCCTTTA
    GATAATAGTTTATTGAGAAAAGAACAAAATGATACGACAGATAAAGATAAGACATCTAAC
    GAGAACAGTGATTCAACTAATAACAGTGATTCCAGCAATCAACAACAACCTGCTACAGAT
    CAAAACTCAAATCAAAATCAAGGTGGCACACAACAAGCGCCACAAGCTTCAAATAACCAA
    AATGGTGTTGTAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1218

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0958 [new locus tag: SAUSA300_RS05150 ]
  • symbol: SAUSA300_0958
  • description: hypothetical protein
  • length: 405
  • theoretical pI: 6.43652
  • theoretical MW: 45684.9
  • GRAVY: -0.687901

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions Other cell envelope-related function transcriptional attenuator common domain (TIGR00350; HMM-score: 115.1)
  • TheSEED  :
    • Cell envelope-associated transcriptional attenuator LytR-CpsA-Psr, subfamily F2 (as in PMID19099556)
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  Cell envelope-associated transcriptional attenuator LytR-CpsA-Psr, subfamily F2 (as in PMID19099556)
  • PFAM:
    no clan defined LytR_cpsA_psr; Cell envelope-related transcriptional attenuator domain (PF03816; HMM-score: 142.2)
    and 8 more
    Baculo_11_kDa; Baculovirus 11 kDa family (PF06143; HMM-score: 15.4)
    DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 14.7)
    SCIMP; SCIMP protein (PF15050; HMM-score: 14.4)
    DUF2140; Uncharacterized protein conserved in bacteria (DUF2140) (PF09911; HMM-score: 14.2)
    Ctr; Ctr copper transporter family (PF04145; HMM-score: 10.5)
    C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 9.2)
    no clan defined DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 8.7)
    Peptidase_AD (CL0130) Presenilin; Presenilin (PF01080; HMM-score: 5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: LFKTSQDA
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.443771
    • TAT(Tat/SPI): 0.007387
    • LIPO(Sec/SPII): 0.044122
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNANRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQIRMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFVEAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRGQRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIKTAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSLIPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQAPQASNNQNGVVN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

[1]

  1. ↑ Kaitlin Schaefer, Leigh M Matano, Yuan Qiao, Daniel Kahne, Suzanne Walker
    In vitro reconstitution demonstrates the cell wall ligase activity of LCP proteins.
    Nat Chem Biol: 2017, 13(4);396-401
    [PubMed:28166208] [WorldCat.org] [DOI] (I p)    Ting Pan, Jing Guan, Yujie Li, Baolin Sun
    LcpB Is a Pyrophosphatase Responsible for Wall Teichoic Acid Synthesis and Virulence in Staphylococcus aureus Clinical Isolate ST59.
    Front Microbiol: 2021, 12;788500
    [PubMed:34975809] [WorldCat.org] [DOI] (P e)
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