PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0925 [new locus tag: NWMN_RS05180 ]
• pan locus tag^?: SAUPAN003264000
• symbol: NWMN_0925
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0925 [new locus tag: NWMN_RS05180 ]
• symbol: NWMN_0925
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1025971..1027188
• length: 1218
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330558 NCBI
• RefSeq: YP_001331959 NCBI
• BioCyc:
• MicrobesOnline: 3706476 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
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  1201
  
  ATGAATAAATTTTTAAAATACTTTTTGATCCTTCTAGCATTGGTTCTCATTGTTGTTCCA
  ATAGTCTTCGCTACGTTACTTTTTAAAACGTCCCAAGATGCATTCGAATCTTCTCAAGAC
  AGCAAAAATGCTAATCGTCAATCCAACTTAAGAGATAATAAAGTCAATCCCGAAGAACAA
  CCTATCTCTATTCTTTTCTTAGGTATCGATGATAACGATGGAAGAAGAAAAAAAGGACAG
  GATGCTGAACACTCTAGATCAGACGCTATGATTTTAACTACTTTTAATCAATCAAAACAT
  CAAATTAGAATGCTTAGTATTCCACGTGATACTATCAGCTACATTCCTAAAGTTGGCTAT
  TACGATAAGATTACCCATGCACATGCATATGGTGGTCCAATTGCCGCAATGGATTCGGTA
  GAAGCTACCATGAATGTACCTGTTGATTATTATGTGAGAGTCAATATGAAAGCATTTGTC
  GAGGCTGTAAATGAGCTTGGTGGCATATACTATGATGTGCCATACGACTTAAATGAACCT
  AACACAGATGATACTGGTAAGATTAAAATTAAAAAAGGGTACCAAAAATTAAATGGTGAT
  GAGGCTTTAGCAGTTGCTAGAACTAGACATCATGATTCAGACTTAAAACGTGGACAACGT
  CAAATGGAATTAATTAAGATTTTATTCCAAAAAGCACAGGAAGTTGATTCTATTGATAAA
  CTTGATAACGTGATTCAAATTGTAGGTAAAAATGCAAAGCATAATTTAACTAACTCTGAA
  ATTAAAGCTTTAGCAAAAATGTACTTAACAAATGATGTTGAAATTAAAACTGCGCAATTA
  AAAGGTAAAGATGATATGTTAAATGGTATTTACTATTATCATCCAAGTGTTGAAAGCATT
  CAAAAATATGCAAACTTACTTCGTAAAGACTTAGAATTATCACCTATTAATGATAAAAAT
  GATTTCTTAGATCAACGTGTCATTAATCATTATGGTTCATTAATACCATTAACGCCTTTA
  GATAATAGTTTATTGAGAAAAGAACAAAATGATACGACAGATAAAGATAAGACATCTAAC
  GAGAACAGTGATTCAACTAATAACAGTGATTCCAGCAATCAACAACAACCTGCTACAGAT
  CAAAACTCAAATCAAAATCAAGGTGGCACACAACAAGCGCCACAAGCTTCAAATAACCAA
  AATGGTGTTGTAAATTAA
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  1218
  

⊟Protein

⊟General

• locus tag: NWMN_0925 [new locus tag: NWMN_RS05180 ]
• symbol: NWMN_0925
• description: hypothetical protein
• length: 405
• theoretical pI: 6.43652
• theoretical MW: 45684.9
• GRAVY: -0.687901

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions Other cell envelope-related function transcriptional attenuator common domain (TIGR00350; HMM-score: 115.1)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined LytR_cpsA_psr; Cell envelope-related transcriptional attenuator domain (PF03816; HMM-score: 142.2)
  and 8 more
  Baculo_11_kDa; Baculovirus 11 kDa family (PF06143; HMM-score: 15.4)
  DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 14.7)
  SCIMP; SCIMP protein (PF15050; HMM-score: 14.4)
  DUF2140; Uncharacterized protein conserved in bacteria (DUF2140) (PF09911; HMM-score: 14.2)
  Ctr; Ctr copper transporter family (PF04145; HMM-score: 10.5)
  C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 9.2)
  no clan defined DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 8.7)
  Peptidase_AD (CL0130) Presenilin; Presenilin (PF01080; HMM-score: 5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.68
  • Cellwall Score: 0.17
  • Extracellular Score: 0.16
  • Internal Helix: 1
• LocateP: Secretory(released) (with CS)
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: LFKTSQDA
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.443771
  • TAT(Tat/SPI): 0.007387
  • LIPO(Sec/SPII): 0.044122
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151221137 NCBI
• RefSeq: YP_001331959 NCBI
• UniProt: A0A0H3K807 UniProt

⊟Protein sequence

• MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNANRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQIRMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFVEAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRGQRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIKTAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSLIPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQAPQASNNQNGVVN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_0924 < NWMN_0925

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications