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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00997
  • pan locus tag?: SAUPAN003264000
  • symbol: SAOUHSC_00997
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00997
  • symbol: SAOUHSC_00997
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 969198..970415
  • length: 1218
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATAAATTTTTAAAATACTTTTTGATCCTTCTAGCATTGGTTCTCATTGTTGTTCCA
    ATAGTCTTCGCTACGTTACTTTTTAAAACGTCCCAAGATGCATTCGAATCTTCTCAAGAC
    AGCAAAAATGCTAATCGTCAATCCAACTTAAGAGATAATAAAGTCAATCCCGAAGAACAA
    CCTATCTCTATTCTTTTCTTAGGTATCGATGATAACGATGGAAGAAGAAAAAAAGGACAG
    GATGCTGAACACTCTAGATCAGACGCTATGATTTTAACTACTTTTAATCAATCAAAACAT
    CAAATTAGAATGCTTAGTATTCCACGTGATACTATCAGCTACATTCCTAAAGTTGGCTAT
    TACGATAAGATTACCCATGCACATGCATATGGTGGTCCAATTGCCGCAATGGATTCGGTA
    GAAGCTACCATGAATGTACCTGTTGATTATTATGTGAGAGTCAATATGAAAGCATTTGTC
    GAGGCTGTAAATGAGCTTGGTGGCATATACTATGATGTGCCATACGACTTAAATGAACCT
    AACACAGATGATACTGGTAAGATTAAAATTAAAAAAGGGTACCAAAAATTAAATGGTGAT
    GAGGCTTTAGCAGTTGCTAGAACTAGACATCATGATTCAGACTTAAAACGTGGACAACGT
    CAAATGGAATTAATTAAGATTTTATTCCAAAAAGCACAGGAAGTTGATTCTATTGATAAA
    CTTGATAACGTGATTCAAATTGTAGGTAAAAATGCAAAGCATAATTTAACTAACTCTGAA
    ATTAAAGCTTTAGCAAAAATGTACTTAACAAATGATGTTGAAATTAAAACTGCGCAATTA
    AAAGGTAAAGATGATATGTTAAATGGTATTTACTATTATCATCCAAGTGTTGAAAGCATT
    CAAAAATATGCAAACTTACTTCGTAAAGACTTAGAATTATCACCTATTAATGATAAAAAT
    GATTTCTTAGATCAACGTGTCATTAATCATTATGGTTCATTAATACCATTAACGCCTTTA
    GATAATAGTTTATTGAGAAAAGAACAAAATGATACGACAGATAAAGATAAGACATCTAAC
    GAGAACAGTGATTCAACTAATAACAGTGATTCCAGCAATCAACAACAACCTGCTACAGAT
    CAAAACTCAAATCAAAATCAAGGTGGCACACAACAAGCGCCACAAGCTTCAAATAACCAA
    AATGGTGTTGTAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1218

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00997
  • symbol: SAOUHSC_00997
  • description: hypothetical protein
  • length: 405
  • theoretical pI: 6.43652
  • theoretical MW: 45684.9
  • GRAVY: -0.687901

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions Other cell envelope-related function transcriptional attenuator common domain (TIGR00350; HMM-score: 115.1)
  • TheSEED  :
    • Cell envelope-associated transcriptional attenuator LytR-CpsA-Psr, subfamily F2 (as in PMID19099556)
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Cell envelope-associated LytR-CpsA-Psr transcriptional attenuators  Cell envelope-associated transcriptional attenuator LytR-CpsA-Psr, subfamily F2 (as in PMID19099556)
  • PFAM:
    no clan defined LytR_cpsA_psr; Cell envelope-related transcriptional attenuator domain (PF03816; HMM-score: 142.2)
    and 8 more
    Baculo_11_kDa; Baculovirus 11 kDa family (PF06143; HMM-score: 15.4)
    DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 14.7)
    SCIMP; SCIMP protein (PF15050; HMM-score: 14.4)
    DUF2140; Uncharacterized protein conserved in bacteria (DUF2140) (PF09911; HMM-score: 14.2)
    Ctr; Ctr copper transporter family (PF04145; HMM-score: 10.5)
    C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 9.2)
    no clan defined DUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 8.7)
    Peptidase_AD (CL0130) Presenilin; Presenilin (PF01080; HMM-score: 5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helix: 1
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: LFKTSQDA
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.443771
    • TAT(Tat/SPI): 0.007387
    • LIPO(Sec/SPII): 0.044122
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNANRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQIRMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFVEAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRGQRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIKTAQLKGKDDMLNGIYYYHPSVESIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSLIPLTPLDNSLLRKEQNDTTDKDKTSNENSDSTNNSDSSNQQQPATDQNSNQNQGGTQQAPQASNNQNGVVN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

S J Foster
Molecular characterization and functional analysis of the major autolysin of Staphylococcus aureus 8325/4.
J Bacteriol: 1995, 177(19);5723-5
[PubMed:7559367] [WorldCat.org] [DOI] (P p)T Oshida, M Sugai, H Komatsuzawa, Y M Hong, H Suginaka, A Tomasz
A Staphylococcus aureus autolysin that has an N-acetylmuramoyl-L-alanine amidase domain and an endo-beta-N-acetylglucosaminidase domain: cloning, sequence analysis, and characterization.
Proc Natl Acad Sci U S A: 1995, 92(1);285-9
[PubMed:7816834] [WorldCat.org] [DOI] (P p)

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