PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1001 [new locus tag: SAUSA300_RS05390 ]
• pan locus tag^?: SAUPAN003325000
• symbol: potC
• pan gene symbol^?: potC
• synonym:
• product: spermidine/putrescine ABC transporter permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1001 [new locus tag: SAUSA300_RS05390 ]
• symbol: potC
• product: spermidine/putrescine ABC transporter permease
• replicon: chromosome
• strand: +
• coordinates: 1096447..1097256
• length: 810
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914674 NCBI
• RefSeq: YP_493699 NCBI
• BioCyc: see SAUSA300_RS05390
• MicrobesOnline: 1292516 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  
  ATGAAATGGTATGGAAAGCTGTATATCGGGATACTTTTAGCGATTTTATACATCCCAATA
  TTCTTTTTAATGTTCTATTCATTCAATTCGGCTGGTAATATGATTCACTTTGAACATTTT
  ACATTAGAGCATTATCAATCATTATTTCAAAATGATCATTTAATGTCGGTCATTTTTAAT
  ACGATAGCTGTAGCACTTTTAGCAGCCTCAATTTCTACAGTTATTGGTACATTTGGTGCC
  ATTGCTATTTATTATTTAAGAAATAAAAAGTTTAAAGTAACTTTACTAACATTGAATAAT
  GTCTTGATGGTATCATCCGACGTTGTCATAGGTGCATCATTCTTAATTATGTTTACAACG
  ATTGGCCATTTTACTGGTCTTGGTTTAGGATTTTGGACAGTTCTAATATCTCATATTGCA
  TTTTGCATACCTATAGTTGTGATTATCGTCTTACCACAACTGTATGAAATGAATAATAAT
  ATGTTAAATGCTGCAAGAGATTTAGGAGCGACTGAACCACAATTATTAAGCAACATTATT
  ATTCCTAATATTTTACCTTCTATTATAGGAGGATTCTTTATGGCTTTAACTTATTCACTA
  GACGATTTCACAGTAAGTTTCTTCGTTACTGGTAATGGCTTTAGTGTGTTATCAGTTGAA
  GTTTATGCTATGGCGAGAAAAGGAATTAGTATGGAAATTAATGCGATTTCAACATTATTG
  TTTGCTGTTATTGTATTAGGAATACTAGGATATTATTTGATTCAATACGTGATAAATAAG
  AAAAAACTAATCAAGCGAGGTGTAAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  810
  

⊟Protein

⊟General

• locus tag: SAUSA300_1001 [new locus tag: SAUSA300_RS05390 ]
• symbol: PotC
• description: spermidine/putrescine ABC transporter permease
• length: 269
• theoretical pI: 9.33859
• theoretical MW: 30002.7
• GRAVY: 0.904089

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 53.1)
  Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, permease protein (TIGR03262; HMM-score: 48.2)
  2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 45.8)
  2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 44.8)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein (TIGR00969; HMM-score: 43.6)
  and 6 more
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 38.8)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 31.4)
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 30.5)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 27.8)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 26.1)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysW (TIGR02140; HMM-score: 25.2)
• TheSEED  :
  • Spermidine/putrescine import ABC transporter, permease subunit PotC
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Polyamine Metabolism  Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1)
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 64.5)
  and 1 more
  no clan defined DUF4293; Domain of unknown function (DUF4293) (PF14126; HMM-score: 8.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 7
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.067434
  • TAT(Tat/SPI): 0.000372
  • LIPO(Sec/SPII): 0.008788
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 87161547 NCBI
• RefSeq: YP_493699 NCBI
• UniProt: A0A0H2XJ31 UniProt

⊟Protein sequence

• MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEHYQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVLMVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNNMLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLSVEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0998 > potA > potB > potC > potD > SAUSA300_1003

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications