PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0998 [new locus tag: SAUSA300_RS05375 ]
• pan locus tag^?: SAUPAN003322000
• symbol: SAUSA300_0998
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0998 [new locus tag: SAUSA300_RS05375 ]
• symbol: SAUSA300_0998
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1094005..1094544
• length: 540
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912885 NCBI
• RefSeq: YP_493696 NCBI
• BioCyc: see SAUSA300_RS05375
• MicrobesOnline: 1292513 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAACATAGGTAATAAAATTAAAAATCTTAGAAGAATTAAAAATTTAACGCAAGAAGAA
  CTTGCTGAACGTACAGACTTATCGAAAGGCTACATTTCACAAATAGAAAGTGAACATGCC
  TCACCAAGTATGGAAACTTTCTTAAATATTATAGAGGTGTTAGGAACGACGCCAAGTGAA
  TTTTTTAAAGACAGTGAAAATGAAAAAGTATTATACAAGAAGGAAGAACAAGTTATTTAT
  GATGAGTATGATGAAGGTTATATATTAAATTGGTTAGTTTCAAAGTCAAATGAATATGAT
  ATGGAGCCATTAATATTAACTTTAAAGCCTGGAGCATCATATAAAAATTTTAATCCATCA
  GAGTCTGATACGTTTATTTATTGTATGTCAGGTCAGATAACACTTAATTTAGGCAAAGAG
  ATATATCAAGCACAAGAAGAAGACGTTTTGTATTTTAAAGCACGAGATAATCATCGTTTG
  TCAAACGAATCAAACAATGAAACACGAATACTTATTGTAGCGACAGCTTCATATTTATAG
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  540
  

⊟Protein

⊟General

• locus tag: SAUSA300_0998 [new locus tag: SAUSA300_RS05375 ]
• symbol: SAUSA300_0998
• description: hypothetical protein
• length: 179
• theoretical pI: 4.42625
• theoretical MW: 20745.1
• GRAVY: -0.602235

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions transcriptional regulator, y4mF family (TIGR03070; HMM-score: 41.7)
  putative allantoin catabolism protein (TIGR03214; HMM-score: 36.2)
  and 6 more
  putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 19)
  Genetic information processing Mobile and extrachromosomal element functions Other addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16.6)
  Signal transduction Regulatory functions DNA interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16.6)
  Signal transduction Regulatory functions Protein interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 16.6)
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 15.8)
  Hypothetical proteins Conserved TIGR00270 family protein (TIGR00270; HMM-score: 13.8)
• TheSEED  :
  • Transcriptional regulator PotR
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Polyamine Metabolism  Transcriptional regulator, MerR family, near polyamine transporter
• PFAM:
  HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 54.3)
  HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 46.4)
  and 13 more
  Cupin (CL0029) Cupin_2; Cupin domain (PF07883; HMM-score: 38.8)
  HTH (CL0123) HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 35.1)
  Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 24.8)
  HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 22.9)
  Cupin (CL0029) Pirin_C; Pirin C-terminal cupin domain (PF05726; HMM-score: 21.8)
  AraC_binding; AraC-like ligand binding domain (PF02311; HMM-score: 20.2)
  HTH (CL0123) HTH_25; Helix-turn-helix domain (PF13413; HMM-score: 18.9)
  no clan defined DUF896; Bacterial protein of unknown function (DUF896) (PF05979; HMM-score: 17.8)
  Cupin (CL0029) CENP-C_C; Mif2/CENP-C like (PF11699; HMM-score: 16.8)
  HTH (CL0123) MarR_2; MarR family (PF12802; HMM-score: 16.3)
  Cupin (CL0029) MannoseP_isomer; Mannose-6-phosphate isomerase (PF01050; HMM-score: 14.7)
  HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 13.2)
  HTH_37; Helix-turn-helix domain (PF13744; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003694
  • TAT(Tat/SPI): 0.000577
  • LIPO(Sec/SPII): 0.000429
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161040 NCBI
• RefSeq: YP_493696 NCBI
• UniProt: A0A0H2XFU9 UniProt

⊟Protein sequence

• MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSMETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDMEPLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEIYQAQEEDVLYFKARDNHRLSNESNNETRILIVATASYL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0998 > potA > potB > potC > potD > SAUSA300_1003

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications