PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1059 [new locus tag: SAUSA300_RS05740 ]
• pan locus tag^?: SAUPAN003426000
• symbol: SAUSA300_1059
• pan gene symbol^?: ssl12
• synonym:
• product: superantigen-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1059 [new locus tag: SAUSA300_RS05740 ]
• symbol: SAUSA300_1059
• product: superantigen-like protein
• replicon: chromosome
• strand: -
• coordinates: 1158771..1159487
• length: 717
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914342 NCBI
• RefSeq: YP_493757 NCBI
• BioCyc: see SAUSA300_RS05740
• MicrobesOnline: 1292574 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAGTAAGAACATCACGAAAAATATAATTTTAACGACAACATTATTACTATTAGGTACT
  GTATTACCTCAAAATCAAAAACCAGTATTTAGTTTTTACTCTGAAGCTAAAGCTTATAGC
  ATTGGTCAAGATGAAACTAACATCAATGAATTAATTAAATATTACACACAGCCTCATTTT
  TCATTTTCAAATAAATGGCTATATCAATATGATAATGGAAACATTTATGTTGAACTTAAG
  AGATATTCATGGTCAGCACATATATCTTTATGGGGCGCTGAAAGTTGGGGAAATATTAAT
  CAGTTAAAAGATCGTTACGTAGATGTGTTTGGACTAAAAGACAAAGATACTGATCAGTTA
  TGGTGGTCTTATAGAGAGACATTTACAGGTGGCGTTACACCAGCCGCAAAACCTTCTGAT
  AAAACTTATAATCTTTTTGTGCAATACAAAGATAAACTACAAACGATTATTGGTGCGCAT
  AAAATATACCAAGGCAATAAACCAGTATTAACATTGAAAGAAATCGATTTCCGTGCACGA
  GAAGCGTTAATAAAAAATAAAATATTATATAACGAAAATCGTAATAAAGGTAAGCTTAAG
  ATCACCGGTGGCGGTAATAACTACACTATTGATTTAAGCAAAAGATTACATTCAGATCTA
  GCAAATGTTTATGTTAAAAATCCTAATAAAATAACTGTTGACGTCCTCTTTGATTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_1059 [new locus tag: SAUSA300_RS05740 ]
• symbol: SAUSA300_1059
• description: superantigen-like protein
• length: 238
• theoretical pI: 9.83237
• theoretical MW: 27568.1
• GRAVY: -0.542857

⊟Function

• TIGRFAM:
• TheSEED  :
  • FIG01107944: hypothetical protein
• PFAM:
  Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 21)
  OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 17.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.01
  • Extracellular Score: 9.97
  • Internal Helices: 0
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 5
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 31 aa
  • SP(Sec/SPI): 0.809367
  • TAT(Tat/SPI): 0.009674
  • LIPO(Sec/SPII): 0.029593
  • Cleavage Site: CS pos: 31-32. VFS-FY. Pr: 0.3442
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161116 NCBI
• RefSeq: YP_493757 NCBI
• UniProt: A0A0H2XFZ2 UniProt

⊟Protein sequence

• MSKNITKNIILTTTLLLLGTVLPQNQKPVFSFYSEAKAYSIGQDETNINELIKYYTQPHFSFSNKWLYQYDNGNIYVELKRYSWSAHISLWGAESWGNINQLKDRYVDVFGLKDKDTDQLWWSYRETFTGGVTPAAKPSDKTYNLFVQYKDKLQTIIGAHKIYQGNKPVLTLKEIDFRAREALIKNKILYNENRNKGKLKITGGGNNYTIDLSKRLHSDLANVYVKNPNKITVDVLFD

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Ilse Jongerius, Jörg Köhl, Manoj K Pandey, Maartje Ruyken, Kok P M van Kessel, Jos A G van Strijp, Suzan H M Rooijakkers
Staphylococcal complement evasion by various convertase-blocking molecules.
J Exp Med: 2007, 204(10);2461-71
[PubMed:17893203] [WorldCat.org] [DOI] (P p)