PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1214 [new locus tag: SAUSA300_RS06585 ]
• pan locus tag^?: SAUPAN003680000
• symbol: SAUSA300_1214
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1214 [new locus tag: SAUSA300_RS06585 ]
• symbol: SAUSA300_1214
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1332379..1333404
• length: 1026
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914276 NCBI
• RefSeq: YP_493911 NCBI
• BioCyc: see SAUSA300_RS06585
• MicrobesOnline: 1292729 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
  
  GTGATTTCATTCGAAAATGATTATTTAGAAGGTGCTCATGAAAAAGTATTAAAACGATTA
  GTAGATACAAATCTTGTACAAGCTTCGGGATATGGCTTTGATCAATTCACAGCTCAGGCA
  ATTGAAAAGATTAAAGATACAATTGACTGTCCAAATGCTACAATTCGTTTTTTAGTAGGT
  GGAACACAAACCAATCAGGTTGTTATTAATTCAATGTTAGAAAGTTACGAAGGGGTCATT
  TCAGCAGACACTGGTCATGTTGCTGTGCATGAGGGCGGTGCAATTGAATATAGTGGACAT
  AAAGTAATTACTATACCCTCTAAGGAAGGAAAAGTTAGTGCTTCAGATGTTGAAACTTAC
  ATGGAGACATTTAAAAGTGATTTTAAAAAGGACCATATGGTATTCCCAGGAATGGTATAT
  ATTTCTCATCCTACAGAATATGGCACATTATACTCTAAATCAGAATTGGAAGAGTTATGC
  AAAGTATGTAAGCAATATCAGCTTCCATTATTTATGGATGGTGCACGATTAGGGTATGGA
  TTGATGAGTGACCAATCAGATATGACTATTAAAGACATCGCTAAATATTGTGATGTATTT
  TATATAGGTGGTACGAAGATAGGTGCATTATGCGGGGAAGCCATTGTTTTTACGAAAAAT
  AATGAACCGAAACAATTTACCACTCGAATCAAGCATCACGGTGCCCTTTTAGCAAAAGGA
  CGGTTAACAGGCATACAATTTTTAGAATTATTTACGGACAATTTATATTTTAATATAAGC
  AGACATGCTATCGAAATGGCAAATAAGATGAAAGACGGGTTTAAAAATAAAGGCTATCGT
  CTTTACTTTGATTCTCCAACCAATCAACAATTTTTTATTTTAAGTAACGAGAAAATAGCA
  GAGTTAGAACAAAAAGTTAAATTTGCTGTTTGGGAAAAATACGATGATCAACATCGTGTT
  GTTAGATTCGCAACCAGTTGGGCGACAACCGAAGAAAATTTAAATAAACTATTAGAATTA
  ATCTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1214 [new locus tag: SAUSA300_RS06585 ]
• symbol: SAUSA300_1214
• description: hypothetical protein
• length: 341
• theoretical pI: 6.18837
• theoretical MW: 38633.8
• GRAVY: -0.293548

⊟Function

• TIGRFAM:
  O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 22.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 20.3)
  and 3 more
  cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 16.7)
  aminotransferase, LLPSF_NHT_00031 family (TIGR04181; HMM-score: 12.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other coenzyme PQQ biosynthesis protein PqqD (TIGR03859; HMM-score: 12.1)
• TheSEED  :
  • Low-specificity L-threonine aldolase (EC 4.1.2.48)
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine Biosynthesis  Low-specificity L-threonine aldolase (EC 4.1.2.48)
  and 1 more
  Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine degradation  Low-specificity L-threonine aldolase (EC 4.1.2.48)
• PFAM:
  PLP_aminotran (CL0061) Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 100.1)
  and 5 more
  Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 25.3)
  Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 22.7)
  SepSecS; O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS (PF05889; HMM-score: 14.1)
  Pyridoxal_deC; Pyridoxal-dependent decarboxylase conserved domain (PF00282; HMM-score: 11.3)
  GHD (CL0369) DUF1945; Domain of unknown function (DUF1945) (PF09178; HMM-score: 8.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.02291
  • TAT(Tat/SPI): 0.001091
  • LIPO(Sec/SPII): 0.000955
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162019 NCBI
• RefSeq: YP_493911 NCBI
• UniProt: A0A0H2XIC2 UniProt

⊟Protein sequence

• MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKIKDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKVITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELCKVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFTKNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKNKGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENLNKLLELI

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications