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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1349 [new locus tag: SACOL_RS06870 ]
  • pan locus tag?: SAUPAN003680000
  • symbol: SACOL1349
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1349 [new locus tag: SACOL_RS06870 ]
  • symbol: SACOL1349
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1356182..1357207
  • length: 1026
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    GTGATTTCATTCGAAAATGATTATTTAGAAGGTGCTCATGAAAAAGTATTAAAACGATTA
    GTAGATACAAATCTTGTACAAGCTTCGGGATATGGCTTTGATCAATTCACAGCTCAGGCA
    ATTGAAAAGATTAAAGATACAATTGACTGTCCAAATGCTACAATTCGTTTTTTAGTAGGT
    GGAACACAAACCAATCAGGTTGTTATTAATTCAATGTTAGAAAGTTACGAAGGGGTCATT
    TCAGCAGACACTGGTCATGTTGCTGTGCATGAGGGCGGTGCAATTGAATATAGTGGACAT
    AAAGTAATTACTATACCCTCTAAGGAAGGAAAAGTTAGTGCTTCAGATGTTGAAACTTAC
    ATGGAGACATTTAAAAGTGATTTTAAAAAGGACCATATGGTATTCCCAGGAATGGTATAT
    ATTTCTCATCCTACAGAATATGGCACATTATACTCTAAATCAGAATTGGAAGAGTTATGC
    AAAGTATGTAAGCAATATCAGCTTCCATTATTTATGGATGGTGCACGATTAGGGTATGGA
    TTGATGAGTGACCAATCAGATATGACTATTAAAGACATCGCTAAATATTGTGATGTATTT
    TATATAGGTGGTACGAAGATAGGTGCATTATGCGGGGAAGCCATTGTTTTTACGAAAAAT
    AATGAACCGAAACAATTTACCACTCGAATCAAGCATCACGGTGCCCTTTTAGCAAAAGGA
    CGGTTAACAGGCATACAATTTTTAGAATTATTTACGGACAATTTATATTTTAATATAAGC
    AGACATGCTATCGAAATGGCAAATAAGATGAAAGACGGGTTTAAAAATAAAGGCTATCGT
    CTTTACTTTGATTCTCCAACCAATCAACAATTTTTTATTTTAAGTAACGAGAAAATAGCA
    GAGTTAGAACAAAAAGTTAAATTTGCTGTTTGGGAAAAATACGATGATCAACATCGTGTT
    GTTAGATTCGCAACCAGTTGGGCGACAACCGAAGAAAATTTAAATAAACTATTAGAATTA
    ATCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1026

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1349 [new locus tag: SACOL_RS06870 ]
  • symbol: SACOL1349
  • description: hypothetical protein
  • length: 341
  • theoretical pI: 6.18837
  • theoretical MW: 38633.8
  • GRAVY: -0.293548

⊟Function[edit | edit source]

  • TIGRFAM:
    O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 22.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 20.3)
    and 3 more
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 16.7)
    aminotransferase, LLPSF_NHT_00031 family (TIGR04181; HMM-score: 12.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other coenzyme PQQ biosynthesis protein PqqD (TIGR03859; HMM-score: 12.1)
  • TheSEED  :
    • Low-specificity L-threonine aldolase (EC 4.1.2.48)
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine Biosynthesis  Low-specificity L-threonine aldolase (EC 4.1.2.48)
    and 1 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine degradation  Low-specificity L-threonine aldolase (EC 4.1.2.48)
  • PFAM:
    PLP_aminotran (CL0061) Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 100.1)
    and 5 more
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 25.3)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 22.7)
    SepSecS; O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS (PF05889; HMM-score: 14.1)
    Pyridoxal_deC; Pyridoxal-dependent decarboxylase conserved domain (PF00282; HMM-score: 11.3)
    GHD (CL0369) DUF1945; Domain of unknown function (DUF1945) (PF09178; HMM-score: 8.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.02291
    • TAT(Tat/SPI): 0.001091
    • LIPO(Sec/SPII): 0.000955
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKIKDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKVITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELCKVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFTKNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKNKGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENLNKLLELI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell: 401 [3]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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