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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1279 [new locus tag: SAUSA300_RS06945 ]
  • pan locus tag?: SAUPAN003793000
  • symbol: phoU
  • pan gene symbol?: phoU
  • synonym:
  • product: phosphate transport system regulatory protein PhoU

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1279 [new locus tag: SAUSA300_RS06945 ]
  • symbol: phoU
  • product: phosphate transport system regulatory protein PhoU
  • replicon: chromosome
  • strand: -
  • coordinates: 1407757..1408398
  • length: 642
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCAATAATTAGACAACGATATCAGGAGCAACTTGATGATTTAATAAAAGAATTACGT
    CGGTTAGGTGCAAATGTCTATGTGAGTATTGAAAATGGTATAAAATCATTAAGTATTGAC
    GATAGAGGCTTTGCACGACAAACAGTTAAAAACGATAAACATATCAATCAATTAAATTAT
    GATATTAATGAGCGAGTTATCATGTTAATTACAAAGCAACAGCCCATTGCGAGTGATTTG
    CGTATGATGATTTCTTCATTAAAAATCGCCTCCGATTTAGAAAGAATAGGAGATAATGCC
    TCGAGTATTGCCAATATTCGATTGCGTACAAAGATTACAGATGATTATGTGTTAACCCGT
    TTAAAGACAATGGGTAAATTAGCTATGTTAATGTTAAAGGACTTAGATCAAGCATTTAAA
    AAGAAAGATACCGTATTAATAAGAGAAATAATTGAGCGTGATGAAGATATCGATGACTTA
    TATAGTCATATTATTAACGCAACGTATCTTATTGATAACGATCCATTTGTCGCTGCACAA
    GCTCATTTAGCAGCAAGACATTTAGAACGTATTGGTGATCATATTATTAACATCGCTGAA
    AGTGTTTATTTTTATTTAACAGGTACACATTACGAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    642

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1279 [new locus tag: SAUSA300_RS06945 ]
  • symbol: PhoU
  • description: phosphate transport system regulatory protein PhoU
  • length: 213
  • theoretical pI: 6.5234
  • theoretical MW: 24619.1
  • GRAVY: -0.284507

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions phosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 225.6)
    Signal transduction Regulatory functions Other phosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 225.6)
  • TheSEED  :
    • Phosphate transport system regulatory protein PhoU
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory High affinity phosphate transporter and control of PHO regulon  Phosphate transport system regulatory protein PhoU
    and 1 more
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Phosphate transport system regulatory protein PhoU
  • PFAM:
    PhoU (CL0297) PhoU; PhoU domain (PF01895; HMM-score: 125)
    and 2 more
    no clan defined Bclx_interact; Bcl-x interacting, BH3 domain (PF08945; HMM-score: 10)
    Met_repress (CL0057) SeqA_N; SeqA protein N-terminal domain (PF17206; HMM-score: 8.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006684
    • TAT(Tat/SPI): 0.000812
    • LIPO(Sec/SPII): 0.000704
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGFARQTVKNDKHINQLNYDINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASSIANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDLYSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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