COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1407 [new locus tag: SAUSA300_RS07675 ]
• pan locus tag^?:
• symbol: SAUSA300_1407
• pan gene symbol^?: —
• synonym:
• product: phi SLT ORF 145-like protein, phage transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1407 [new locus tag: SAUSA300_RS07675 ]
• symbol: SAUSA300_1407
• product: phi SLT ORF 145-like protein, phage transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 1572658..1573095
• length: 438
• essential: unknown

⊟Accession numbers

• Gene ID: 3912772 NCBI
• RefSeq: YP_494104 NCBI
• BioCyc: see SAUSA300_RS07675
• MicrobesOnline: 1292922 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGGGAACACAATATATGATATCAAGCCAGGCACATTTAAATATATTGAATCAGAAATA
  TATAATTTAAATGAGAACAAGAAAGAAATAAAAAGATTGAGGTTGGAAATACTTAATCCA
  ACGAAGGAACAAGATTCCAATATTGTATATGGACCATTACAAAAAGGCGAACCAGTTAGA
  ACAACTGAACTAATGGCAACTAGATTATTAACTAATAAGATGTTACGAAACCTTGAAGAA
  ATGGTCGAAGCAGTTGAGAGTGAATACTTAAAGTTGCCTGAAGATCATAAGAAAGTAATA
  AGGCTCAAGTATTGGAATAAAGAAAAGAAGTTAAAGATGGAACAGATAGGACATGAATGC
  CATATGCATCGTAATACTGTTACTACTATACGAAAGAACTTTGTTAAAGCGGTAGCGTAT
  CATGCAGGTATCAAATAA
  60
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  438
  

⊟Protein

⊟General

• locus tag: SAUSA300_1407 [new locus tag: SAUSA300_RS07675 ]
• symbol: SAUSA300_1407
• description: phi SLT ORF 145-like protein, phage transcriptional regulator
• length: 145
• theoretical pI: 9.92753
• theoretical MW: 17158.9
• GRAVY: -0.75931

⊟Function

• TIGRFAM:
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage transcriptional regulator, RinA family (TIGR01636; HMM-score: 61.2)
  Signal transduction Regulatory functions DNA interactions phage transcriptional regulator, RinA family (TIGR01636; HMM-score: 61.2)
  and 2 more
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 20.4)
  RNA polymerase sigma-70 factor, Planctomycetaceae-specific subfamily 1 (TIGR02984; HMM-score: 13.1)
• TheSEED  :
  • Phage integrase regulator RinA
• PFAM:
  HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 17.2)
  Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 16.4)
  Rep (CL0169) Relaxase; Relaxase/Mobilisation nuclease domain (PF03432; HMM-score: 15.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00478
  • TAT(Tat/SPI): 0.000228
  • LIPO(Sec/SPII): 0.000518
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161062 NCBI
• RefSeq: YP_494104 NCBI
• UniProt: A0A0H2XHZ6 UniProt

⊟Protein sequence

• MGNTIYDIKPGTFKYIESEIYNLNENKKEIKRLRLEILNPTKEQDSNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRLKYWNKEKKLKMEQIGHECHMHRNTVTTIRKNFVKAVAYHAGIK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1407 < SAUSA300_1408 < SAUSA300_1409

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications