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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1408 [new locus tag: SAUSA300_RS07680 ]
  • pan locus tag?: SAUPAN001768000
  • symbol: SAUSA300_1408
  • pan gene symbol?:
  • synonym:
  • product: phage helicase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1408 [new locus tag: SAUSA300_RS07680 ]
  • symbol: SAUSA300_1408
  • product: phage helicase
  • replicon: chromosome
  • strand: -
  • coordinates: 1573108..1574379
  • length: 1272
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGGTCTAGGGAAAACAGTATCAACACTTACAGCATTTAGTGAATTGCAGTTGTTAGAC
    ACTAAAAAAATGTTAGTTATAGCACCTAAACAAGTTGCTAAAGATACATGGGTTGATGAA
    GTTGATAAGTGGAACCATTTAAATCATCTGAAAGTGTCTTTAGTTTTAGGAACACCTAAA
    GAAAGAAATGATGCATTAAACACAGAGGCTGATATCTATGTAACCAATAAAGAAAATACT
    AAATGGTTATGTGATCAATATAAAAAAGAATGGCCATTTGACATGGTTGTGATTGATGAA
    CTGTCTACATTTAAAAGTCCTAAGAGTCAAAGGTTTAAATCTATTAAAAAGAAATTACCA
    CTCATTAATAGATTTATAGGATTAACAGGAACACCTAGTCCAAATAGTTTACAGGATTTA
    TGGGCTCAAGTTTATTTGATAGACAGAGGTGAAAGACTTGAGTCTTCATTCAGTCGTTAT
    CGAGAAAGGTACTTTAAACCAACTCATCAAGTTAGCGAACATATTTTTAAGTGGGAGCTA
    AGAGACGGATCTGAAGAAAAGATATATAAACAAATAGAAGATATATGTTTAAGCATGAAA
    GCGAAAGATTATCTGGATATGCCTGACAGAGTTGATACTAAACAAACAGTAGTCTTATCA
    GAAAAAGAAAGAAAAGTATATGAAGAATTAGAAAAAAACTATATTTTAGAATCGGAAGAA
    GAAGGAACAGTTGTAGCTCAAAATGGGGCATCATTAAGTCAGAAACTACTTCAACTATCT
    AACGGCGCAGTTTATACAGATGAGGAAGATGTAAGACTTATACATGATAAGAAGTTAGAT
    AAGTTAGAGGAAATTATAGAGGAGTCTCAAGGCCAACCAATACTATTGTTTTATAACTTC
    AAACACGATAAAGAAAGAATACTTCAAAGGTTTAAGGAAGCAACCACATTAGACGATTCA
    AACTATAAAGAACGTTGGAACAGTGGAGACATTAAGTTGCTTATAGCACATCCAGCAAGT
    GCAGGACATGGATTAAACTTACAACAAGGTGGGCACATTATTGTTTGGTTTGGACTTACA
    TGGTCCTTGGAATTATACCAACAAGCAAATGCTAGATTATACAGACAAGGACAAAATCAT
    ACGACTATTATTCATCATATCATGACCGATAACACAATAGATCAAAGAGTATATAAAGCT
    TTACAAAATAAAGAACTAACGCAAGAAGAATTAATGAAAGCTATTAAAGCAAGAATAGCT
    AAGCATAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1272

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1408 [new locus tag: SAUSA300_RS07680 ]
  • symbol: SAUSA300_1408
  • description: phage helicase
  • length: 423
  • theoretical pI: 7.20915
  • theoretical MW: 49310.9
  • GRAVY: -0.650118

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • DNA helicase, phage-associated
  • PFAM:
    P-loop_NTPase (CL0023) SNF2_N; SNF2 family N-terminal domain (PF00176; HMM-score: 73.5)
    and 2 more
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 23.4)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 22)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009331
    • TAT(Tat/SPI): 0.000487
    • LIPO(Sec/SPII): 0.001089
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MGLGKTVSTLTAFSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEADIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGLTGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEEKIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTVVAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQPILLFYNFKHDKERILQRFKEATTLDDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLTWSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKARIAKHK

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]