PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1494 [new locus tag: SAUSA300_RS08155 ]
• pan locus tag^?: SAUPAN004110000
• symbol: SAUSA300_1494
• pan gene symbol^?: lipM
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1494 [new locus tag: SAUSA300_RS08155 ]
• symbol: SAUSA300_1494
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1647044..1647874
• length: 831
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914729 NCBI
• RefSeq: YP_494189 NCBI
• BioCyc: see SAUSA300_RS08155
• MicrobesOnline: 1293009 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACTGAAACTTGGAATTTTATTAATACAGGAAGTAAAGACCCATATTATAATATGGCA
  ATGGATGAAGCGTTATTAAATTTTGTCTCTAGAGGTGAAATTGATCCAGTTATACGTTTT
  TACACATGGAATCCTGCAACATTATCAATAGGGTATTTCCAAAGGCTACAAAAAGAAATT
  GATATTGATAAGGTTAAAGAGAAAGGTTTTGGTCTCGTAAGGCGTCAAACTGGTGGCCGT
  GGGGTGCTACATGATAAAGAGTTAACTTACAGTGTGATTGTGCCTGAATCGCATCCGAAT
  ATGCCTTCTACAGTTACAGAAGCGTACAGAGTGATTTCACAAGGATTATTAGAAGGATTT
  AAAAACTTAGGATTTGATACTTATTTTGCGGTACCTAAAACGCCTGAAGAGCGTCAAAAA
  TTAAAACAACCTCGAAGTTCAGTGTGTTTTGATGCACCAAGTTGGTATGAGCTAGTTGTT
  GAAGGCCGTAAGATTGCTGGGAGTGCTCAAACGAGACAAAAGGGCGTAATCCTCCAACAC
  GGTTCGATTTTACAAGATATTGATATTGATGAACTGTTTGATATGTTTATTTATAAAAAT
  GAAAGATTAAAATTAAAAATGAAAGAGGCATTCGTTGAAAAAGCAGTTGCCATCAATGAT
  ATTTCAGATGAACACATTACTATTAGTCAAATGGAAGAAGCTTTCGAGAAAGGTTTTAAG
  AAAGGTTTAAATATAGAATTTAAGCCACTTGAATTAACAGAAGCTCAATTGGCAGAAGTG
  GAAGAATTAACTGAAAAATATCGTTCAGATGAATGGATGTTTAGAAAGTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_1494 [new locus tag: SAUSA300_RS08155 ]
• symbol: SAUSA300_1494
• description: hypothetical protein
• length: 276
• theoretical pI: 5.15375
• theoretical MW: 31915.2
• GRAVY: -0.477536

⊟Function

• reaction:
  EC 2.3.1.181?  ExPASy
  Lipoyl(octanoyl) transferase Octanoyl-[acyl-carrier-protein] + protein = protein N⁶-(octanoyl)lysine + [acyl-carrier-protein]
• TIGRFAM:
  Genetic information processing Protein fate Protein modification and repair lipoyltransferase and lipoate-protein ligase (TIGR00545; HMM-score: 100.5)
• TheSEED  :
  • Octanoate-[acyl-carrier-protein]-protein-N-octanoyltransferase, LipM
  Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Lipoate-protein ligase A
  and 1 more
  Cofactors, Vitamins, Prosthetic Groups, Pigments Lipoic acid Lipoic acid metabolism  Lipoate-protein ligase A
• PFAM:
  tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 95.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002278
  • TAT(Tat/SPI): 0.000151
  • LIPO(Sec/SPII): 0.000409
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160517 NCBI
• RefSeq: YP_494189 NCBI
• UniProt: A0A0H2XFB3 UniProt

⊟Protein sequence

• MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWNPATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMPSTVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVVEGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAINDISDEHITISQMEEAFEKGFKKGLNIEFKPLELTEAQLAEVEELTEKYRSDEWMFRK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications