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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1531 [new locus tag: SAUSA300_RS08350 ]
- pan locus tag?: SAUPAN004153000
- symbol: phoH
- pan gene symbol?: phoH
- synonym:
- product: PhoH family phosphate starvation-induced protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1531 [new locus tag: SAUSA300_RS08350 ]
- symbol: phoH
- product: PhoH family phosphate starvation-induced protein
- replicon: chromosome
- strand: -
- coordinates: 1680718..1681665
- length: 948
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914322 NCBI
- RefSeq: YP_494226 NCBI
- BioCyc: see SAUSA300_RS08350
- MicrobesOnline: 1293046 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901ATGCCTGGAATTATACAAATAGACGATATGAACCAATCTCAAGCTTTAATTGGAAATAAT
GATGAACATTTAAAAGCAATTGAAGAGAGTTTCGATGTTGTCATCCATGCAAGAGGACAA
GAAGTTGCCGTTAAAGGTACAAAAATAGAAAACGTAGAAAAAGCGGAATCAGTATTAATC
AATTTGCTGAAGGTTATTGATTTAGGTAATAATATTACAATTAAAGATGTTGAAGCAGCT
ATTAAAATGGCGCATAATAACACAATTCAACATCTGTTAGATTTATATGATGAAGAGATA
ACTAAAGATGCATTTGGTAAGACGATTCGTGCGAAAACGATGGGGCAACGTATATATGTT
AATGCCATGAAAAATAATGATTTAGTATTTGGTATAGGTCCTGCTGGTACAGGTAAGACA
TTCTTAGCTGTAGTTTATGCAGCAAAGCAACTCCGTAAAGGTGCTGTTAAACGTATTGTA
TTAACAAGACCTGCTGTTGAAGCAGGAGAGTCACTTGGATTTTTACCAGGAGATTTGAAA
GAAAAGGTAGATCCATATTTAAGACCTTTATATGATGGTCTATATACTGTTCTTGGGCGT
GAACAAACAGAGCGATTTATTGAAAGAGGCATTATCGAAATAGCGCCACTTGCATATATG
CGCGGACGAACATTAGAAGATGCATTTGTAATTCTTGATGAGGCGCAGAATACGACACAT
GCGCAAATGAAAATGTTTTTAACAAGACTAGGTTTTGGCTCAAAAATGGTAGTTACTGGT
GACCAAACTCAAATCGATTTACCTAAAGGTGTTAAAAGTGGACTTAAGGAAGCGGTCAGT
AGGTTACACAACGTTAAAGGTATAAGTATATTGAAATTAGATCAGAGCGATGTAGTCAGA
CATCCATTGGTAAGTAAGATCATTGAACATTATGAAGGAGAGAATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1531 [new locus tag: SAUSA300_RS08350 ]
- symbol: PhoH
- description: PhoH family phosphate starvation-induced protein
- length: 315
- theoretical pI: 6.98088
- theoretical MW: 34913.1
- GRAVY: -0.165714
⊟Function[edit | edit source]
- TIGRFAM: DNA metabolism DNA replication, recombination, and repair putative DNA helicase (TIGR00376; EC 3.6.4.12; HMM-score: 17)
- TheSEED :
- PhoH-like protein, PhoL
- PFAM: P-loop_NTPase (CL0023) PhoH; PhoH-like protein (PF02562; HMM-score: 314.7)and 10 moreAAA_30; AAA domain (PF13604; HMM-score: 35)AAA_19; AAA domain (PF13245; HMM-score: 24.8)AAA_22; AAA domain (PF13401; HMM-score: 18.9)KH (CL0007) KH_1; KH domain (PF00013; HMM-score: 18)P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 16.8)UvrD-helicase; UvrD/REP helicase N-terminal domain (PF00580; HMM-score: 13.9)DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 13.5)Zot; Zonular occludens toxin (Zot) (PF05707; HMM-score: 13.5)no clan defined Vps16_C; Vps16, C-terminal region (PF04840; HMM-score: 12.7)P-loop_NTPase (CL0023) AAA_25; AAA domain (PF13481; HMM-score: 12.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002182
- TAT(Tat/SPI): 0.000143
- LIPO(Sec/SPII): 0.000319
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAVKGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITKDAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIVLTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLAYMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLKEAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: recO < era < cdd < dgkA < SAUSA300_1530 < phoH
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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